Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

Berlina, Anna N.; Ragozina, Maria Y.; Komova, Nadezhda S.; Serebrennikova, Kseniya V.; Zherdev, Anatoly V.; Dzantiev, Boris B.

doi:10.3390/bios12111002

Cited by 9 publications

(5 citation statements)

References 60 publications

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“…To show the performance and applicability of this method, it was decided to limit ourselves to a set of three groups of samples that differ in chemical composition. Thus, spring water and water from a waterfall were chosen as pure natural objects that were previously used in our research [25,33] and were categorized into the first group. Except for water, PAEs, including DiBP, are also found in seafood as well as in other aquatic life [13].…”

Section: Analysis Of Samples Obtained By Various Extraction Methodsmentioning

confidence: 99%

“…For the development of ELISA, hapten-protein conjugates were obtained. As described in our previous work [25], a protein conjugate with diisobutyl phthalate was obtained using its amino derivative by an azo coupling reaction. Soybean trypsin inhibitor (STI) and bovine serum albumin (BSA) were used as carrier proteins.…”

Section: Rabbit Immunization and Antisera Obtainingmentioning

confidence: 99%

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Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

et al. 2023

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The use of plasticizers to improve the quality of plastics widely used for household purposes inevitably leads to an increase in their pollution of food and environmental objects. Diisobutyl phthalate (DiBP) is one of the ortho-substituted phthalic acid esters that negatively affect human health and ecosystems. This work is directed to the development of a chemiluminescent enzyme immunoassay (CL-ELISA) for the determination of diisobutyl phthalate in water and food. Luminol, which is oxidized with hydrogen peroxide in the presence of p-iodophenol as an enhancer, was chosen as the substrate for horseradish peroxidase used as a label in the analysis. For this development, rabbit anti-DiBP polyclonal antibodies were generated and tested with the synthesized hapten–protein conjugate. The developed chemiluminescent ELISA has a detection limit of 1.8 ng/mL; the operating range was 5.0–170.8 ng/mL at a content of 10% methanol in the assay medium. The assay was successfully applied to detect diisobutyl phthalate in lettuce leaves, seafood, and water. When using extraction with methanol and hexane, the recovery of DiBP in samples varies in the range of 76.9–134.2%; for assays in natural waters, the recovery rates are from 79.5 to 113.4%.

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Section: Analysis Of Samples Obtained By Various Extraction Methodsmentioning

confidence: 99%

Section: Rabbit Immunization and Antisera Obtainingmentioning

confidence: 99%

Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

et al. 2023

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“…Colorimetric characterization is very important for image high-fidelity display, colorimetric measurement, color reproduction, color analysis, color management of different devices [ 5 ] and color appearance prediction [ 6 ], and the requirements for the accuracy and stability of color characterization are increasing for different applications. Color analysis [ 7 , 8 , 9 , 10 ] plays a very important role in the evaluation of the performance of many technologies including paper-based devices that use colorimetric reactions and that researchers have used with respect to different color spaces such as weighted RGB, greyscale, etc.…”

Section: Introductionmentioning

confidence: 99%

Colorimetric Characterization of Color Imaging System Based on Kernel Partial Least Squares

Zhao

Liu

Zhao

et al. 2023

Sensors

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Colorimetric characterization is the basis of color information management in color imaging systems. In this paper, we propose a colorimetric characterization method based on kernel partial least squares (KPLS) for color imaging systems. This method takes the kernel function expansion of the three-channel response values (RGB) in the device-dependent space of the imaging system as input feature vectors, and CIE-1931 XYZ as output vectors. We first establish a KPLS color-characterization model for color imaging systems. Then we determine the hyperparameters based on nested cross validation and grid search; a color space transformation model is realized. The proposed model is validated with experiments. The CIELAB, CIELUV and CIEDE2000 color differences are used as evaluation metrics. The results of the nested cross validation test for the ColorChecker SG chart show that the proposed model is superior to the weighted nonlinear regression model and the neural network model. The method proposed in this paper has good prediction accuracy.

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“…Immunoassay methods have great advantages over chromatographic methods due to their relatively low cost and implementation simplicity. For DBP determination, there are commonly used immunological techniques such as enzyme-linked immunosorbent assay (ELISA) [39][40][41], immunochromatographic (ICA) [42] and fluorescence polarization immunoassay (FPIA) [43,44].…”

Section: Introductionmentioning

confidence: 99%

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay

Mukhametova,

Karimova,

Zharikova

et al. 2023

Biosensors

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Dibutyl phthalate (DBP) is widely used as a plasticizer in the production of polymeric materials to give them flexibility, strength and extensibility. However, due to its negative impact on human health, in particular reproductive functions and fetal development, the content of DBP must be controlled in food and the environment. The present study aims to develop a sensitive, fast and simple fluorescence polarization immunoassay (FPIA) using monoclonal antibodies derived against DBP (MAb-DBP) for its detection in open waters. New conjugates of DBP with various fluorescein derivatives were obtained and characterized: 5-aminomethylfluorescein (AMF) and dichlorotriazinylaminofluorescein (DTAF). The advantages of using the DBP-AMF conjugate in the FPIA method are shown, the kinetics of binding of this chemical with antibodies are studied, the analysis is optimized, and the concentration of monoclonal antibodies is selected for sensitivity analysis—16 nM. The calibration dependence of the fluorescence polarization signal for the detection of DBP was obtained. The observed IC50 (DBP concentration at which a 50% decrease in the fluorescence polarization signal occurs, 40 ng/mL) and the limit of detection (LOD, 7.5 ng/mL) values were improved by a factor of 45 over the previously described FPIA using polyclonal antibodies. This technique was tested by the recovery method, and the high percentage of DBP discovery in water ranged from 85 to 110%. Using the developed method, real water samples from Lake Onega were tested, and a good correlation was shown between the results of the determination of DBP by the FPIA method and GC-MS. Thus, the FPIA method developed in this work can be used to determine DBP in open-water reservoirs.

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Development of Lateral Flow Test-System for the Immunoassay of Dibutyl Phthalate in Natural Waters

Cited by 9 publications

References 60 publications

Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

Colorimetric Characterization of Color Imaging System Based on Kernel Partial Least Squares

Detection of Dibutyl Phthalate in Surface Water by Fluorescence Polarization Immunoassay

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