Development of liquid crystal biosensor for the detection of amyloid beta-42 levels associated with Alzheimer's disease

Kemiklioğlu, Emine; Tuncgovde, Ebru Busra; Özşarlak-Sözer, Gönen

doi:10.1016/j.jbiosc.2021.03.016

Cited by 16 publications

(6 citation statements)

References 40 publications

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“…Our incubation duration for the antibody is 1 hour, which is notably shorter than the typical incubation time of 8 hours used by other LC droplets and LC-based biosensors for amyloid beta 1-42 detection. 43 In a different biosensor developed by our lab, the response time has been dependent on droplet size and protein content. 51,52 This emphasizes the importance of optimization processes in biosensor development.…”

Section: Discussionmentioning

confidence: 99%

“…52 In LC droplet biosensing, two primary sensing mechanisms are predominantly utilized: electrostatic interactions between the LC surfaces and biomolecules, and the immunoassay approach involving antibody-antigen (ligandreceptor) interactions. 41,43 These mechanisms have been successfully applied in the sensing of proteins, bacteria, viruses, bile acids, and disease detection, showcasing the versatility and efficacy of LC droplet-based biosensing technologies. [36][37][38][39] In this study, we have engineered LC droplets by coating them with a mixture of polyethyleneimine (PEI) and Tween-20 surfactant, followed by immobilizing anti-beta amyloid 1-16 antibodies on the surface of these droplets.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Label-free detection of Aβ-42: a liquid crystal droplet approach for Alzheimer's disease diagnosis

Pradhan,

Pathinti,

Kandimalla

et al. 2024

RSC Adv.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Label-free detection of Aβ-42: a liquid crystal droplet approach for Alzheimer's disease diagnosis

Pradhan,

Pathinti,

Kandimalla

et al. 2024

RSC Adv.

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show abstract

“…Afterward, Apolipoprotein E4 (ApoE4), another risk factor for AD, was added into the system to form a triadic complex, changing the optical textures and reflectance of the LC film. The lowest concentration of Ab42 that could be detected was 1 pg/mL and the detection range of ApoE4 was 0.1–30 nM through this system [ 93 ].…”

Section: Applications In Lc Biochemical Sensorsmentioning

confidence: 99%

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

et al. 2022

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As an emerging stimuli-responsive material, liquid crystal (LC) has attracted great attentions beyond display applications, especially in the area of biochemical sensors. Its high sensitivity and fast response to various biological or chemical analytes make it possible to fabricate a simple, real-time, label-free, and cost-effective LC-based detection platform. Advancements have been achieved in the development of LC-based sensors, both in fundamental research and practical applications. This paper briefly reviews the state-of-the-art research on LC sensors in the biochemical field, from basic properties of LC material to the detection mechanisms of LC sensors that are categorized into LC-solid, LC–aqueous, and LC droplet platforms. In addition, various analytes detected by LCs are presented as a proof of the application value, including metal ions, nucleic acids, proteins, glucose, and some toxic chemical substances. Furthermore, a machine-learning-assisted LC sensing platform is realized to provide a foundation for device intelligence and automatization. It is believed that a portable, convenient, and user-friendly LC-based biochemical sensing device will be achieved in the future.

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“…Among optical sensing approaches, liquid crystal (LC) biosensors have become an emerging research field for cancer biomarkers evaluation in recent years 39 – 43 . LCs are a prospective branch of excellent sensing and phase transition materials that exist between isotropic liquids and highly ordered solid crystals, hence LCs are mentioned as intermediate phases or mesophases 44 – 46 . They have the ability to flow like fluids and also exhibit anisotropic peculiarities such as the birefringence of crystalline materials 47 , 48 .…”

Section: Introductionmentioning

confidence: 99%

Liquid crystal-assisted optical biosensor for early-stage diagnosis of mammary glands using HER-2

Pourasl

Vahedi

Tajalli

et al. 2023

Sci Rep

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Breast cancer (BC) is one of the most commonly diagnosed cancers and the second leading cause of cancer mortality among women around the world. The purpose of this study is to present a non-labeled liquid crystal (LC) biosensor, based on the inherent feature of nematic LCs, for the evaluation of BC using the human epidermal growth factor receptor-2 (HER-2) biomarker. The mechanism of this sensing is supported by surface modification with dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) encouraging the long alkyl chains that induce a homeotropic orientation of the LC molecules at the interface. To enhance the binding efficacy of more HER-2 antibody (Ab) on LC aligning agents, a simple ultraviolet radiation-assisted method was also used to increase functional groups on the DMOAP coated slides, thereby improving binding affinity and efficiency onto HER-2 Abs. The designed biosensor makes use of the specific binding of HER-2 protein to HER-2 Ab and disruption of the orientation of LCs. This orientation change leads to a transition of the optical appearance from dark to birefringent, enabling the detection of HER-2. This novel biosensor exhibits a linear optical response to HER-2 concentration in the wide dynamic range of 10–6–102 ng/mL, with an ultra-low detection limit of 1 fg/mL. As a proof of concept, the designed LC biosensor was successfully investigated for the quantification of HER-2 protein in patients suffering from BC. Owing to the sensitivity, selectivity, and label-free detection, this biosensor may amplify the application of LC-based biosensors for the detection of most types of cancers.

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Development of liquid crystal biosensor for the detection of amyloid beta-42 levels associated with Alzheimer's disease

Cited by 16 publications

References 40 publications

Label-free detection of Aβ-42: a liquid crystal droplet approach for Alzheimer's disease diagnosis

Label-free detection of Aβ-42: a liquid crystal droplet approach for Alzheimer's disease diagnosis

State-of-the-Art Development in Liquid Crystal Biochemical Sensors

Liquid crystal-assisted optical biosensor for early-stage diagnosis of mammary glands using HER-2

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