Development of long-term primary cell culture of Macrobrachium rosenbergii: morphology, metabolic activity, and cell-cycle analysis
Gurucharan Sudarshan,
Simy Weil,
Rivka Manor
et al.
Abstract:This study describes our attempts to generate a sustainable cell culture of Macrobrachium rosenbergii. We present here a continuous longitudinal study on the embryonic primary cell culture of freshwater prawn M. rosenbergii that was uniquely monitored for up to 90 days with regard to its morphology, metabolic activity, and cell-cycle parameters. The daily monitoring of cells’ wellbeing and morphology showed seeded cells to be changing from attached singular diverse-sized cells after days 4–10 to interconnected… Show more
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