Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis

Alhassan, Andy; Thekisoe, Oriel; Yokoyama, Naoki; Inoue, Noboru; Motloang, Makhosazana; Mbati, Peter A.; Yin, Hong; Katayama, Yoshinari; Anzai, Toru; Sugimoto, Chihiro; Igarashi, Ikuo

doi:10.1016/j.vetpar.2006.08.014

Cited by 74 publications

(43 citation statements)

References 20 publications

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“…Other common enteric pathogens, such as E. coli O157, Vibrio cholerae, Salmonella enterica serovar Typhi, and Cryptosporidium parvum, were tested and showed no LAMP amplification (data not shown). Thus, the results indicate that the E. histolytica LAMP can detect E. histolytica with high specificity, in accordance with previous studies showing extremely high specificity of LAMP assays in the detection of protozoan parasites, such as trypanosomes, Babesia gibsoni, and those causing equine piroplasmosis (1,13,15).…”

supporting

confidence: 91%

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica

Liang¹,

Chan

Hsia³

et al. 2009

J Clin Microbiol

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A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the results compared with those of conventional nested PCR to validate the practicability of this assay. No DNA was amplified from other diarrheal pathogens, such as other Entamoeba species, bacteria, and viruses. These results indicate that LAMP is a rapid, simple, and valuable diagnostic tool for epidemiological studies of amebiasis.

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supporting

confidence: 91%

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica

Liang¹,

Chan

Hsia³

et al. 2009

J Clin Microbiol

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“…LAMP assay can be evaluated as a suitable method for rapidly spreading infection diseases which is important early diagnosis and use under field conditions. PCR method is inhibited with DNA polymerase inhibitors in water samples [14] while, LAMP assay does not affect any inhibitors in water samples [15].…”

Section: Discussionmentioning

confidence: 99%

LAMP DETECTION of Cryptosporidium SPECIES in SURFACE WATER SAMPLES COLLECTED from RIVER YESILIRMAK and STREAM TERSAKAN (Samsun-Amasya)

Kolören¹

2017

ANADOLU UNIVERSITY JOURNAL OF SCIENCE AND TECHNOLOGY –C Life Sciences and Biotechnology

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“…EMA and PMA have been reported to combine with LAMP for detection of viable cells of Salmonella [106][107][108][109]. The concentration of EMA used in the assay is critical in affecting DNA amplification of viable cells.…”

Section: Ema/pma-lampmentioning

confidence: 99%

Recent and the Latest Developments in Rapid and Efficient Detection of Salmonella in Food and Water

Hu¹,

Li²

2017

Adv Tech Biol Med

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Salmonella is a leading cause of foodborne diseases. Gastroenteritis caused by nontyphoid Salmonella is still a major infectious disease in the world. About 95% of Salmonella infections are caused by ingestion of contaminated food and water. Rapid, sensitive, and efficient detection and identification of Salmonella from foods and water are critical for minimizing the spread of outbreaks caused by this pathogen. These methods can be applied to track the food source of contamination or by early diagnosis of the infections in a clinical setting. Culture-based methods for detection of Salmonella are laborious and time-consuming, typically taking 5-7 days to obtain a pure culture and serovar identification. In the past few decades, molecular-based technologies have greatly shortened the time for detection and identification of bacterial pathogens from food and water, and drastically increased the specificity and sensitivity of the assays. In this review, we report an update on the development of rapid and efficient methods for the detection and identification of Salmonella in food and water, focusing on the approaches for concentration of Salmonella cells and viable cell detection, as well as the advantages and drawbacks of these methods.

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Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis

Cited by 74 publications

References 20 publications

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica

Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica

LAMP DETECTION of Cryptosporidium SPECIES in SURFACE WATER SAMPLES COLLECTED from RIVER YESILIRMAK and STREAM TERSAKAN (Samsun-Amasya)

Recent and the Latest Developments in Rapid and Efficient Detection of Salmonella in Food and Water

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