Development of mast cells from grafted bone marrow cells in irradiated mice

Kitamura, Yukihiko; Shimada, M.; Hatanaka, Kenji; Miyano, Yasuyuki

doi:10.1038/268442a0

Cited by 277 publications

(168 citation statements)

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“…Origin of mast cell precursors were determined by using giant granules of bgJ/bg J mice as a marker (22,24). In one experiment, bone marrow cells of C57BL/6-bgJ/bg J mice were injected intravenously to nonirradiated WBB6F1-W/W v mice.…”

Section: Assay Of Spleen Colony-forming Unit (Cfu-s) the Methods Of Tmentioning

confidence: 99%

“…Mice of (WB × C57BL/6)F~ (hereafter WBB6F~)-(W/W ~, W/+, WV/+, +/+) and C57BL/6-(bgJ/bg y, +/+) were raised in our laboratory. Giant granules of C57BL/6-bgJ/ bg J mice were used as a marker to identify the origin of mast cells (23,24). Original stocks of mutant mice were derived from The Jackson Laboratory, Bar Harbor, ME, but W v and bg J mutant genes have been maintained in C57BL/6 mice of our own inbred colony (more than 28 and 15 backcrosses, respectively at the time of the present experiment) (25,26).…”

Section: Methodsmentioning

confidence: 99%

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Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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Presence of mast cell precursors in the mouse peritoneal cavity was demonstrated, and the precursors were characterized. When a cell suspension, containing mast cell precursor(s), was directly injected into the skin of genetically mast cell-deficient WBB6F1 (WB X C57BL/6)-W/Wv mice, a cluster composed of approximately 2,000 mast cells appeared at the injection site. By determining the proportion of injection sites at which the mast cell cluster appeared, the concentration of mast cell precursors can be calculated by limiting dilution analysis. The concentration in the peritoneal cavity was about five times as great as the concentration in the bone marrow. Although peritoneal mast cell precursors were shown to originate from the bone marrow, physical characterization revealed that the peritoneal precursors differed from the marrow precursors. The peritoneal precursors were less susceptible to irradiation than the marrow precursors; the former were heavier than the latter. When a 95% pure mast cell suspension was prepared from the peritoneal cells by the removal of phagocytes and the density gradient centrifugation, 1 out of 16 cells had the potentiality to make a mast cell cluster in the skin of the W/Wv mice. Moreover, when a single mast cell was identified under the phase contrast microscope and picked up with the micromanipulator, 1 out of 17 mast cells made the cluster. This indicated that some peritoneal mast cells kept extensive proliferative potentiality even after morphological differentiation. In other words, some peritoneal mast cells themselves may function as the committed precursors.

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Section: Assay Of Spleen Colony-forming Unit (Cfu-s) the Methods Of Tmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Sonoda¹,

Kanayama²,

Hara³

et al. 1984

The Journal of Experimental Medicine

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“…In the present study, we adopted a different strategy: we injected cultured mast cells directly into mast cell-deficient (WB X (34,35). The original stocks of mutant mice were obtained from The Jackson Laboratory, Bar Harbor, ME, but W v and bg J mutant genes have been maintained in C57BL/6 mice of our own inbred colony (more than 29 and 16 backcrosses, respectively, at the time of the present experiments) (36,37).…”

mentioning

confidence: 99%

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

Nakano¹,

Sonoda²,

Hayashi³

et al. 1985

The Journal of Experimental Medicine

471

268

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Both connective tissue mast cells and mast cells grown in vitro are derived from multipotential hematopoietic stem cells, but these two mast cell populations exhibit many differences in morphology, biochemistry, and function. We investigated whether the phenotype of cultured mast cells or their progeny was altered when the cells were transferred into different locations in vivo. Cultured mast cells were immature by ultrastructure, and stained with alcian blue but with neither safranin or berberine sulfate, a fluorescent dye that binds to the heparin of connective tissue mast cell granules. By contrast, mast cells recovered from the peritoneal cavity of congenitally mast cell-deficient (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mice 10 wk after intraperitoneal injection of cultured WBB6F1-+/+ or C57BL/6-bgJ/bgJ mast cells stained with both safranin and berberine sulfate. Staining with berberine sulfate was prevented by treatment of the cells with heparinase but not chondroitinase ABC, suggesting that the adoptively transferred mast cell population had acquired the ability to synthesize and store heparin. Furthermore, the recovered mast cells were indistinguishable by ultrastructure from the normal mature peritoneal mast cells of WBB6F1-+/+ mice, and contained substantially more histamine than mast cells studied directly from culture. Intravenous injection of cultured mast cells resulted in the development of safranin-and berberine sulfate-positive mast cells in the peritoneal cavity, spleen, skin, and glandular stomach muscularis propria. Mast cells also developed on the glandular stomach mucosa, but these cells stained with alcian blue rather than safranin, and did not stain with berberine sulfate. This result suggests that cultured mast cells can give rise to mast cells of either the connective tissue type or mucosal phenotype, depending on anatomical location. Furthermore, transplantation of cultured mast cells into WBB6F1-W/Wv mice had no measurable effect on the anemia of the recipient mice, suggesting a possible strategy for repairing the mast cell deficiency of WBB6F1-W/Wv mice without affecting other bone marrow-derived populations such as erythrocytes. Intravenous injection of representative connective tissue type mast cells (30-50% pure peritoneal mast cells derived from WBB6F1-+/+ mice) gave results similar to those obtained with cultured mast cells: mast cells developing in the peritoneal cavity, skin, spleen, and glandular stomach muscularis propria of WBB6F1-W/Wv recipients stained with safranin and berberine sulfate, whereas mast cells developing in the mucosa of the glandular stomach stained only with alcian blue.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Mast cells distribute in various systemic organs and tissues, e.g., the respiratory mucosa, skin dermis, or dura mater (14)(15)(16)(17)(18)(19). In such tissues, they tend to exist near or in contact with neurites of nerve cells (20); electron microscopic observation has revealed that the intercellular distance is generally <20 nm (21,22).…”

Section: Introductionmentioning

confidence: 99%

Time-Course Statistical Evaluation of Intercellular Adhesion Maturation by Femtosecond Laser Impulse

Iino

Hagiyama

Furuno

et al. 2016

Biophysical Journal

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The maturation of intercellular adhesion is an essential process for establishing the signal transduction network in living cells. Although the maturation is naturally considered to enhance the signal transduction, the relationship between the signal transduction and the maturation process has not been revealed in detail using time-course data. Here, using a coculture of mast cells and neurites, differences in maturation between individual cells were estimated as a function of the adhesion strength by our original single-cell measurement method utilizing a laser-induced impulsive force. When an intense femtosecond laser is focused into a culture medium under a microscope, shock and stress waves are generated at the laser focal point that exert an impulsive force on individual cells. In our method, this impulse is used to break the adhesion between a mast cell and a neurite. The magnitude of the impulse is then quantified by a local force-measurement system utilizing an atomic force microscope, and the adhesion strength is estimated from the threshold of the impulse required to break the adhesion. The measurement is conducted within 1 min/cell, and thus, data on the individual differences of the adhesion strength can be obtained within only a few hours. Coculturing of neurites and mast cells for 4 h resulted in a specific adhesion that was stronger than the nonspecific adhesions between the substrate and mast cells. In the time-course investigation, we identified two distinct temporal patterns of adhesion: 1) the strength at 24 h was the same as the initial strength; and 2) the strength increased threefold from baseline and became saturated within 24 h. Based on these results, the distribution of CADM1 adhesion molecules in the neurites was suggested to be inhomogeneous, and the relationship between adhesion maturation and the signal-transduction process was considered.

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Development of mast cells from grafted bone marrow cells in irradiated mice

Cited by 277 publications

References 8 publications

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Proliferation of peritoneal mast cells in the skin of W/Wv mice that genetically lack mast cells.

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells.

Time-Course Statistical Evaluation of Intercellular Adhesion Maturation by Femtosecond Laser Impulse

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