Development of Membrane Filter with Oligosaccharide Immobilized by Click Chemistry for Influenza Virus Adsorption

Abstract

Abstract:Membranes with immobilized GM3-type oligosaccharide were prepared and influenza virus adsorption was evaluated. GM3-type oligosaccharide which has azido group in the aglycon was synthesized by saccharide primer method. The azido group was used for combination by click chemistry with alkyne which is introduced to membranes. The position of the azido group in the aglycon of GM3-type oligosaccharide was either the farthest (12-azidododecyl), or the nearest (2-azidododecyl). The azido group in the farthest position showed higher reactivity with alkyne on the membrane than the one in the nearest the GM3-type oligosaccharide. Moreover, the membrane immobilized GM3-type oligosaccharide showed adsorption ability of influenza virus.

Carbohydrate-Functionalized Chitosan Fiber for Influenza Virus Capture

“…Further expanded bioevaluations, including the virus-capture test in a nonaqueous environment, are currently being pursued. Overall, although different kinds of sugar-incorporated systems have been studied as probes for detections of pathogens and carbohydrate–protein interactions, − the above results reveal first that direct modification of a fibrous material with glyco-functionality can generate a unique system for virus decontamination.…”

“…The second is a simple filtration device featured by a sheet of SL-modified chitosan fiber (SLCF 2 ) to effectively remove the virus from the medium. Sugar-incorporated systems reported in the past are diverse with various functions, which include conjugated polymers and dendrimers, nanoparticles, vesicles, magnetic beads, quantum dots, membrane, and microarrays. − To the best of our knowledge, however, this work is the first to functionalize a fibrous material to confer the molecular recognition capability with the sugar. Importantly, the use of polysaccharide chitosan, which has been widely used in the pharmaceutical and medical areas due to its unique properties, such as biocompatibility, biodegradability, and antimicrobial activity, − as the scaffold enables not only the improved safety of SLCC 1 as a potential anti-influenza agent, but also attractive applications of SLCF 2 as a filter material in the control of the virus transmission.…”

Designing syntheses of cellulose and starch derivatives with basic or cationic N‐functions: part I—cellulose derivatives

“…In a similar approach, Kato et al . attached oligosaccharides on the surface of filter paper (Scheme ) . In the first step, cellulose was treated with bromoacetic acid in the presence of aqueous NaOH.…”

“…Preparation of cellulose membrane with immobilized saccharide units by click chemistry reaction . This figure is available in colour online at wileyonlinelibrary.com/journal/pat…”

“…A number of innovative applications have also been developed by other researchers for such derivatives including preparation of highly active filter materials, chemical library generation, catalyst in organic reactions, membrane filter for influenza virus adsorption, deodorant, heavy metal adsorbents, and flocculant . However, most of these works are preliminary studies.…”