Development of Methodology for Detection of Formaldehyde-DNPH in Milk Manager by Central Composite Rotational Design and GC/MS

Abstract

Milk is among the food more consumed by humanity. In this way, a study of its physical-chemical characteristics is justified to analyze if its components are by the legislation and if its hygiene conditions, more precisely the use of formaldehyde as a preservative, are established. The International Agency for Research on Cancer warns that formaldehyde has physicochemical properties that make it carcinogenic when consumed in food. In Brazil, the legislation does not establish minimum values for the presence of formaldehyde in foods of animal origin, and the technique suggested by the Ministry of Agriculture, Livestock, and Supply is a qualitative test. With the large consumption of milk by the Brazilian population and because it is a food subject to adulteration. The present work sought to analyze milk in terms of its physical-chemical characteristics (fat, density, freezing point, non-greasy solids, lactose, and proteins) and develop a methodology to analyze formaldehyde derivatized with 2,4-dinitrophenylhydrazine in milk samples using gas chromatography coupled to mass spectrometer.

A New Perspective on SPME and SPME Arrow: Formaldehyde Determination by On-Sample Derivatization Coupled with Multiple and Cooling-Assisted Extractions

“…Other derivatizing reagents are available on the market, such as 2,4-dinitrophenylhydrazine (DNPH) and 2,2,2-trifluoroethylhydrazine (TFEH). However, although DNPH derivatization affords low limits of detection and low chemical noise in the blank samples [ 38 ], hydrazones must be extracted or preconcentrated before analysis [ 42 , 43 , 44 ]. As for TFEH, it could be used to form the TFE–hydrazone [ 44 , 45 ], but the reagent is more expensive than PFBHA.…”

Pengaruh Supporting Electrolyte Pada Penentuan Formaldehida Secara Voltametri Siklik Menggunakan Pencil Lead Electrode (PLE) Modifikasi Lapisan Tipis Perak

“…Karena potensi toksisitasnya, World Health Organization (WHO) telah menetapkan ambang batas paparan formaldehida pada manusia tidak boleh lebih dari 0,08 mg/L dalam jangka waktu 30 menit [4]. Umumnya, metode yang digunakan dalam mendeteksi kandungan formaldehida adalah Spektrofotometri UV-VIS [5], High Performance Liquid Chromatografi (HPLC) [6], dan Gas Chromatography (GC) [7]. Namun penggunaan metode ini memiliki beberapa kelemahan seperti biaya instrumentasi yang mahal, proses persiapan sampel yang rumit, dan waktu analisis yang cukup lama.…”