Development of methods for determination of phenolic acids and flavonoids in capsules containing Corylus avellana L. Dry extract

Abstract

The questions of standardization and quality control of both herbs and herbal remedies remain relevant, because it is well-known that product quality standards are essential, whether consumer using herbs or drugs. The necessity of the standardization methods development for the initial herbal material and capsule dosage form for the further quality control under manufacturing conditions remains relevant. Aim. The aim of our research was to develop simple, specific, accurate and reproducible methods for identification of flavonoids and phenolic acids in capsule dosage form containing Corylus avellana L. dry extract. Methods. The samples of gelatine capsules containing Corylus avellana L. dry extract for oral administration were analyzed. The analysis was carried out using Camag HPTLC system. The absorption spectroscopy determination of the sum of flavonoids was carried out using THERMO Scientific Evolution 60S Spectroscope in wavelength range of 300-600 nm. Results. As a result of HPTLC research rutine and quercitrine have been identified in capsule dosage form containing Corylus avellana L. dry extract. Among phenolic acids, neochlorogenic and chlorogenic acids have been identified. Under the given conditions, the spectrum of the test solution had a maximum absorption at wavelength 406 nm. The analysis of flavonoids total content in gelatine capsules containing Corylus avellana L. dry extract calculated as rutine has shown the content of 1,7 %. Conclusion. Effective HPTLC and absorption spectroscopy methods for determination of flavonoids and phenolic acids in capsule dosage form containing Corylus avellana L. dry extract have been developed. It has been found that described methods are promising enough for standardization of capsules with Corylus avellana L. dry extract and may be suggested for the quality control of the dosage form under manufacturing conditions