Development of Micro-Flow-Controlled Techniques and Novel Stationary Phases in Capillary Liquid Chromatography

Abstract

This focusing review summarizes our recent works on the dissemination of capillary liquid chromatography through various approaches, i.e. simple stepwise-gradient elution system, on-line sample enrichment system, alternate-pumping recycle chromatography system, on-line immobilized-enzyme reactor, development of novel stationary phases, etc. Capillary liquid chromatography saves energy and waste, and it possesses several advantages over the conventional liquid chromatography; however, it has not been much popularized since its appearance and most users still prefer to use conventional liquid chromatography if there is a choice between them. Our research focuses on, but not limited to, developing versatile capillary liquid chromatography systems that are not only environmental-friendly but also user-friendly, and novel stationary phases that could be operated under mixed modes conditions by manipulating the eluent, which are applicable to real samples analyses and will therefore eventually switch the users from "conventional" to "capillary".

One-pot Synthesis of Polyethylene glycol-based Polymeric Monolithic Stationary Phases for Capillary Ion Chromatography

“…One of the main challenges in making monolithic stationary phase is the column preparation repeatability [12]. Therefore, the PEGmonolithic capillary columns were prepared (under optimized conditions) and the separation efficiency was evaluated repeatedly in this study.…”

“…Similarly, silica-based monoliths, which are prepared through the sol-gel method using alkoxysillanes, generally show high mechanical strength and excellent separation performance compared to organic polymer-based monoliths, which are prepared by reacting a solution comprising copolymerized monomer(s), a crosslinking agent, porogen(s) (pore-forming agent), and a polymerization initiator [11,12]. The organic polymer-type monolithic columns are easier to prepare.…”

“…The organic polymer-type monolithic columns are easier to prepare. Moreover, they show excellent pH durability, which is more suitable to be used as stationary phases for IC applications [12].…”

Investigation of Chromatographic Performance of Hyperbranched Amine-Modified Stationary Phases in Ion Chromatography

“…There are many kinds of stationary phases which are chemically or dynamically modified with quaternary ammonium [3][4][5][6][7][8].…”