Development of MRI/NIRF ‘activatable’ multimodal imaging probe based on iron oxide nanoparticles

Joon, Eui; Jang, Eue Soon; Sun, In Cheol; Lee, In Joon; Ko, Jeong Hoon; Kim, Young Il; Kwon, Ick Chan; Kim, Kwangmeyung; Ahn, Cheol Hee

doi:10.1016/j.jconrel.2011.07.019

Cited by 55 publications

(45 citation statements)

References 30 publications

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“…Actually, prepared Fe 3 O 4 @SiO 2 nanoparticles with a diameter of 79 and 95 nm precipitate even after only a few hours in water; however, Fe 3 O 4 @SiO 2 nanoparticles with a 1.0 ± 0.5 nm thick silica layer were mono-dispersed in solvent for a few weeks without any evidence of aggregation. 24 Therefore, a thin silica coating is preferred for the present magnetofection study, and Fe 3 O 4 @SiO 2 nanoparticles with a 1.0 ± 0.5 nm silica layer were used for gene transfection in this study.…”

Section: Resultsmentioning

confidence: 99%

“…Actually, the PEI-conjugated Fe 3 O 4 @SiO 2 nanoparticles (PEI-Fe 3 O 4 @SiO 2 ) were remarkably stable for a couple of weeks without aggregation or sedimentation. 24 The PEI-Fe 3 O 4 @SiO 2 nanoparticles were used as gene delivery vector for a human fibroblast cell (IMR-90) line. Although the transfection efficiency of the PEI-Fe 3 O 4 @SiO 2 nanoparticles is very low, it was increased remarkably by adding Lipofectamine 2000.…”

Section: Introductionmentioning

confidence: 99%

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Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

Jang¹,

Park²

2012

Bulletin of the Korean Chemical Society

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We successfully synthesized Fe 3 O 4 @SiO 2 nanoparticles with ultrathin silica layer of 1.0 ± 0.5 nm that polyethyleneimine (PEI) with low molecular weight of 2.0-4.0 kDa was covalently conjugated with the resulting Fe 3 O 4 @SiO 2 nanoparticles by silane coupling reaction. The PEI-Fe 3 O 4 @SiO 2 nanoparticles were further used as gene delivery vector for a human fibroblast cell (IMR-90) line. Gene transfection efficiency of the PEI-Fe 3 O 4 @SiO 2 complexes did not increase remarkably after magnetofection; however, the addition of Lipofectamine 2000 significantly increased the transfection efficiency of the PEI-Fe 3 O 4 @SiO 2 complexes. We believe that the present approach could be utilized for magnetofection as alternative to Fe 3 O 4 nanoparticles conjugated with the PEI of high molecular weight thanks to its relatively low cytotoxicity and high transfection efficiency.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

Jang¹,

Park²

2012

Bulletin of the Korean Chemical Society

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“…29 Nevertheless, due to the short interval between the emitting species and the magnetic one, fluorescence of the fluorophore would be strongly reduced or even quenched. 15,24,30 To solve this problem, Cha et al designed an "activatable" multimodal imaging probe. In their study, the NIR fluorescent dye Cy5.5 was connected to the SPIO through a matrix metalloproteinase substrate.…”

Section: Discussionmentioning

confidence: 99%

“…In their study, the NIR fluorescent dye Cy5.5 was connected to the SPIO through a matrix metalloproteinase substrate. 24 In a normal physiological environment, the Cy5.5 was quenched by the SPIO. At the tumor site, the substrate was hydrolyzed by matrix metalloproteinase 2.…”

Section: Discussionmentioning

confidence: 99%

Glioma-targeting micelles for optical/magnetic resonance dual-mode imaging

Zhou¹,

Mu²,

Jiang³

et al. 2015

IJN

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Surgical resection is the primary mode for glioma treatment, while gross total resection is difficult to achieve, due to the invasiveness of the gliomas. Meanwhile, the tumor-resection region is closely related to survival rate and life quality. Therefore, we developed optical/magnetic resonance imaging (MRI) bifunctional targeted micelles for glioma so as to delineate the glioma location before and during operation. The micelles were constructed through encapsulation of hydrophobic superparamagnetic iron oxide nanoparticles (SPIONs) with polyethylene glycol- block -polycaprolactone (PEG- b -PCL) by using a solvent-evaporation method, and modified with a near-infrared fluorescent probe, Cy5.5, in addition to the glioma-targeting ligand lactoferrin (Lf). Being encapsulated by PEG- b -PCL, the hydrophobic SPIONs dispersed well in phosphate-buffered saline over 4 weeks, and the relaxivity ( r 2 ) of micelles was 215.4 mM −1 ·s −1 , with sustained satisfactory fluorescent imaging ability, which might have been due to the interval formed by PEG- b -PCL for avoiding the fluorescence quenching caused by SPIONs. The in vivo results indicated that the nanoparticles with Lf accumulated efficiently in glioma cells and prolonged the duration of hypointensity at the tumor site over 48 hours in the MR image compared to the nontarget group. Corresponding with the MRI results, the margin of the glioma was clearly demarcated in the fluorescence image, wherein the average fluorescence intensity of the tumor was about fourfold higher than that of normal brain tissue. Furthermore, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide assay results showed that the micelles were biocompatible at Fe concentrations of 0–100 μg/mL. In general, these optical/MRI bifunctional micelles can specifically target the glioma and provide guidance for surgical resection of the glioma before and during operation.

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“…6,7 Thus, biocompatible materials such as gold, iron oxide, and silica nanoparticles are becoming choice of materials for biomedical applications. 1,2,8 Among them, gold nanorods (GNRs) grown anisotropically along the [001] direction have been extensively studied during the past decade because of their biocompatibility and excellent plasmonic responses. 9,10 In particular, longitudinal surface plasmon resonance (LSPR) of free electrons along the elongated axis of the GNR can be quickly converted to thermal energy through the lattice collisions, a feature that has recently been recognized to be highly promising for cancer therapy.…”

Section: -5mentioning

confidence: 99%

Critical Enhancement of Photothermal Effect by Integrated Nanocomposites of Gold Nanorods and Iron Oxide on Graphene Oxide

Yun¹,

Seo²,

Kim³

et al. 2013

Bulletin of the Korean Chemical Society

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Irradiation of gold nanorods (GNRs) with laser light corresponding to the longitudinal surface plasmon oscillation results in rapid conversion of electromagnetic energy into heat, a phenomenon commonly known as the photothermal effect of GNRs. Herein, we propose a facile strategy for increasing the photothermal conversion efficiency of GNRs by integration to form graphene oxide (GO) nanocomposites. Moreover, conjugation of iron oxide (IO) with the GO-GNR nanohybrid allowed magnetic enrichment at a specific target site and the separated GO-IO-GNR assembly was rapidly heated by laser irradiation. The present GO-IO-GNR nanocomposites hold great promise for application in various biomedical fields, including surface enhanced Raman spectroscopy imaging, photoacoustic tomography imaging, magnetic resonance imaging, and photothermal cancer therapy.

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Development of MRI/NIRF ‘activatable’ multimodal imaging probe based on iron oxide nanoparticles

Cited by 55 publications

References 30 publications

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

Glioma-targeting micelles for optical/magnetic resonance dual-mode imaging

Critical Enhancement of Photothermal Effect by Integrated Nanocomposites of Gold Nanorods and Iron Oxide on Graphene Oxide

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Development of MRI/NIRF ‘activatable’ multimodal imaging probe based on iron oxide nanoparticles

Cited by 55 publications

References 30 publications

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe3O4@SiO2Nanoparticles

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe3O4@SiO2Nanoparticles

Glioma-targeting micelles for optical/magnetic resonance dual-mode imaging

Critical Enhancement of Photothermal Effect by Integrated Nanocomposites of Gold Nanorods and Iron Oxide on Graphene Oxide

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Product

Resources

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Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles

Lipofectamine-2000 Assisted Magnetofection to Fibroblast Cells Using Polyethyleneimine-Fe₃O₄@SiO₂Nanoparticles