Development of multiplex <scp>PCR</scp> system with 15 <scp>X</scp>‐<scp>STR</scp> loci and genetic analysis in three nationality populations from <scp>C</scp>hina

Liu, Qiuling; Lu, Dalei; Quan, Li; Chen, Ye-Fei; Shen, Min; Zhao, Hu

doi:10.1002/elps.201100558

Cited by 21 publications

(9 citation statements)

References 30 publications

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“…For the comparison between the Hui group and the three groups from Asia, we found four loci (DXS7424, DXS6789, DXS981, and GATA31E08) of the seven comparative loci in Japanese ; two loci (DXS7423 and DXS6789) of the six comparative loci in Korean ; and one locus (DXS6789) of the three comparative loci in Philippine were in significant differences. Statistically significant differences were observed between the Hui group and Chinese Taiwanese at two loci (DXS6789 and DXS101); Zhejiang Han population at four STR loci (DXS7133, DXS6789, DXS101, and DXS10164); Guangdong Han population at four STR loci (DXS7133, DXS981, DXS6789 and DXS7423); Uigur group at three STR loci (DXS7133, DXS981 and DXS7423); Kazakh group at four STR loci (DXS7133, DXS981, DXS6789 and GATA31E08); Ewenki group at four STR loci (DXS7133, DXS8378, DXS10159 and DXS7423); Oroqen group at two loci (DXS8378 and DXS10159); Daur group at three loci (DXS8378, DXS10159, and DXS7423); Luoba group at four loci (DXS8378, DXS6789, DXS10159, and DXS7423); Naxi group at four loci (DXS8378, DXS6789, DXS10159, and GATA31E08); and Hui group in Ningxia at one locus (GATA31E08). The compared results showed no significant differences between our studied group and Mongolian at the eight comparative loci.…”

Section: Resultsmentioning

confidence: 95%

Diversity study of 12 X‐chromosomal STR loci in Hui ethnic from China

et al. 2014

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X-chromosomal STRs (X-STRs) have been used as complements of autosomal STR application in recent years. In this work, we present population genetic data of 12 X-STRs including DXS101, DXS10159, DXS10162, DXS10164, DXS6789, DXS7133, DXS7423, DXS7424, DXS8378, DXS981, GATA165B12, and GATA31E08 loci in a sample of 231 unrelated healthy individuals from the Hui ethnic group in Ningxia Hui Autonomous Region, China. Allelic frequencies of the 12 X-STR loci and haplotypic frequencies of the reported linkage groups (DXS7424-DXS101 and DXS10159-DXS10164-DXS10162) were investigated in the group, respectively. No STR loci showed significant deviations from the Hardy-Weinberg equilibriums and no linkage disequilibriums of pairwise loci were found after Bonferroni correction, respectively. A combined power of discrimination in female individuals was 0.999999999985 and that in male individuals was 0.99999967, respectively. The combined mean exclusion chance in deficiency cases, normal trios and duo cases were 0.999934, 0.995754, and 0.999796, respectively. Significant differences were observed from 0 to 8 loci, when making comparisons between the data of Hui ethnic group and previously reported data from other 16 populations. The results indicated the new panel of 12 X-STR loci might be useful for forensic science application.

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Section: Resultsmentioning

confidence: 95%

Diversity study of 12 X‐chromosomal STR loci in Hui ethnic from China

et al. 2014

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“…All samples were genotyped for 26 X‐STR loci in two multiplex systems including MX15‐STR and MX12‐STR. MX15‐STR consisted of DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, DXS6800, GATA31E08, DXS10074, and DXS10079 in a single multiplex reaction, in which primer and PCR conditions were as described elsewhere . MX12‐STR consisted of DXS6854, DXS9902, DXS6800, GATA172D05, DXS7423, HPRTB, DXS6807, DXS6803, DXS6804, DXS6799, DXS8378, and DXS8377 in a single multiplex reaction.…”

Section: Methodsmentioning

confidence: 99%

X chromosomal recombination—A family study analyzing 26 X‐STR Loci in Chinese Han three‐generation pedigrees

Liu

et al. 2013

Electrophoresis

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The aim of this study is to investigate genetic linkage and recombination fractions of 26 X chromosomal (X-STR) loci with two multiplex PCR systems (MX15-STR and MX12-STR). MX15-STR (including DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, DXS6800, GATA31E08, DXS10074, and DXS10079) and MX12-STR (including DXS6854, DXS9902, DXS6800, GATA172D05, DXS7423, HPRTB, DXS6807, DXS6803, DXS6804, DXS6799, DXS8378, and DXS8377) were successful analyzed on 206 two-generation families with two or more children and 33 three-generation families with 72 grandsons. Segregation analysis and calculation of recombination fractions between pairs of markers were performed. Linkage analysis of pairs of markers showed that there existed significant linkage (maximum LOD scores >2.0) within the physical distance of 48.5 Mb. Recombination events could be observed within the clusters of closed linked makers spanning <1.0 Mb. These results indicate that close cluster X-STRs used and recombination fractions of the selected loci will be very useful for biostatistical calculations in complex kinship analysis.

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“…The allele frequencies and further statistical information of the twenty-six loci in Han, Uigur and Mongol population are shown in Table 1. The allele frequencies and further statistical information in Kazakh has been described in MX15-STR [10] and MX12-STR [11]. HWE was performed on female samples, and the P-values of HWE are greater than 0.05 at all the twenty-six loci.…”

Section: Resultsmentioning

confidence: 99%

“…MX15-STR consisted of DXS7133, DXS6801, DXS981, DXS6809, DXS7424, DXS6789, DXS9898, DXS7132, GATA165B12, DXS101, DXS10075, DXS6800, GATA31E08, DXS10074 and DXS10079 in a single multiplex reaction, in which primer and PCR conditions were as described elsewhere [10]. MX12-STR consisted of DXS6854, DXS9902, DXS6800, GATA172D05, DXS7423, HPRTB, DXS6807, DXS6803, DXS6804, DXS6799, DXS8378 and DXS8377 in a single multiplex reaction, in which primer and PCR conditions were as described elsewhere [11].…”

Section: Methodsmentioning

confidence: 99%

Allele and Haplotype Diversity of 26 X-STR Loci in Four Nationality Populations from China

et al. 2013

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BackgroundHaplotype analysis of closely associated markers has proven to be a powerful tool in kinship analysis, especially when short tandem repeats (STR) fail to resolve uncertainty in relationship analysis. STR located on the X chromosome show stronger linkage disequilibrium compared with autosomal STR. So, it is necessary to estimate the haplotype frequencies directly from population studies as linkage disequilibrium is population-specific.Methodology and FindingsTwenty-six X-STR loci including six clusters of linked markers DXS6807-DXS8378-DXS9902(Xp22), DXS7132-DXS10079-DXS10074-DXS10075-DXS981 (Xq12), DXS6801-DXS6809-DXS6789-DXS6799(Xq21), DXS7424-DXS101-DXS7133(Xq22), DXS6804-GATA172D05(Xq23), DXS8377-DXS7423 (Xq28) and the loci DXS6800, DXS6803, DXS9898, GATA165B12, DXS6854, HPRTB and GATA31E08 were typed in four nationality (Han, Uigur, Kazakh and Mongol) samples from China (n = 1522, 876 males and 646 females). Allele and haplotype frequency as well as linkage disequilibrium data for kinship calculation were observed. The allele frequency distribution among different populations was compared. A total of 5–20 alleles for each locus were observed and altogether 289 alleles for all the selected loci were found. Allele frequency distribution for most X-STR loci is different in different populations. A total of 876 male samples were investigated by haplotype analysis and for linkage disequilibrium. A total of 89, 703, 335, 147, 39 and 63 haplotypes were observed. Haplotype diversity was 0.9584, 0.9994, 0.9935, 0.9736, 0.9427 and 0.9571 for cluster I, II, III, IV, V and VI, respectively. Eighty-two percent of the haplotype of cluster IIwas found only once. And 94% of the haplotype of cluster III show a frequency of <1%.ConclusionsThese results indicate that allele frequency distribution for most X-STR loci is population-specific and haplotypes of six clusters provide a powerful tool for kinship testing and relationship investigation. So it is necessary to obtain allele frequency and haplotypes data of the linked loci for forensic application.

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Development of multiplex PCR system with 15 X‐STR loci and genetic analysis in three nationality populations from China

Cited by 21 publications

References 30 publications

Diversity study of 12 X‐chromosomal STR loci in Hui ethnic from China

Diversity study of 12 X‐chromosomal STR loci in Hui ethnic from China

X chromosomal recombination—A family study analyzing 26 X‐STR Loci in Chinese Han three‐generation pedigrees

Allele and Haplotype Diversity of 26 X-STR Loci in Four Nationality Populations from China

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