Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae

Abstract

Ayapana triplinervis is a plant species used in traditional medicine and in mystical-religious rituals by traditional communities in the Amazon. The aim of this study are to develop a nano-emulsion containing essential oil from A. triplinervis morphotypes, to evaluate larvicidal activity against Aedes aegypti and acute oral toxicity in Swiss albino mice (Mus musculus). The essential oils were extracted by steam dragging, identified by gas chromatography coupled to mass spectrometry, and nano-emulsions were prepared using the low energy method. Phytochemical analyses indicated the major compounds, expressed as area percentage, β-Caryophyllene (45.93%) and Thymohydroquinone Dimethyl Ether (32.93%) in morphotype A; and Thymohydroquinone Dimethyl Ether (84.53%) was found in morphotype B. Morphotype A essential oil nano-emulsion showed a particle size of 101.400 ± 0.971 nm (polydispersity index = 0.124 ± 0.009 and zeta potential = -19.300 ± 0.787 mV). Morphotype B essential oil nano-emulsion had a particle size of 104.567 ± 0.416 nm (polydispersity index = 0.168 ± 0.016 and zeta potential = -27.700 ± 1.307 mV). Histomorphological analyses showed the presence of inflammatory cells in the liver of animals treated with morphotype A essential oil nano-emulsion (MAEON) and morphotype B essential oil nano-emulsion (MBEON). Congestion and the presence of transudate with leukocyte infiltration in the lung of animals treated with MAEON were observed. The nano-emulsions containing essential oils of A. triplinervis morphotypes showed an effective nanobiotechnological product in the chemical control of A. aegypti larvae with minimal toxicological action for non-target mammals.

The essential oil ofSchinus terebinthifoliusand its nanoemulsion and isolated monoterpenes: investigation of their activity againstCulex pipienswith insights into the adverse effects on non‐target organisms

“…When transformed into nanoparticles, materials acquire unique physical and morphological characteristics that affect their bioactivities. Doubtless, the subcellular size and large surface area of nanopesticides, including nanoemulsions, promote their affinity towards targeted bio‐systems, their spread, ability to permeating and uptake by bio‐membranes, hence their biological activities are enhanced 14–16 . In the literature, enhanced and promising biological, including mosquitocidal, activities were obtained when natural plant products, particularly Eos, were extracted and tested at the nanoscale against various harmful insects 14–16, 29, 55–57 .…”

“…Nanopesticides acquire novel physical and biological characteristics such as increased surface area and affinity to the targeted biomembranes, enhancing their biological activities. [14][15][16] Schinus terebinthifolius Raddi (Anacardiaceae), the so-called Brazilian pepper, rose pepper or Christmas berry, is an evergreen monoecious tree indigenous to South and Central America, semitropical regions of the USA, Brazil and Africa. 17 It is one of many oil-bearing plants containing terpenes of various types as biologically active metabolites.…”

“…Nanotechnology can overcome these shortcomings by developing plant‐based nanomaterials and test their pesticidal properties. Nanopesticides acquire novel physical and biological characteristics such as increased surface area and affinity to the targeted bio‐membranes, enhancing their biological activities 14–16 …”

Identification of Potential Insect Growth Inhibitor against Aedes aegypti: A Bioinformatics Approach

“…Accepting that the insect’s physiology is a key point in the development of a new insecticide, the in silico pharmacokinetic properties that have been developed mainly include properties inherent to humans. However, the planning of an insecticide is based on some properties that are shared by other live organisms since the physiology is similar in some physicochemical properties [ 66 , 67 , 68 , 69 ]. In this study, four positive controls (diflubenzuron, DFB, teflubenzuron, TEF, flucycloxuron, FCX, and buprofezin, BUP) were used.…”

Biodiversidade e o Desenvolvimento Local na Comunidade de São Francisco do Iratapurú/Amapá no uso e acesso e repartição de benefícios do conhecimento tradicional associado

“…Nesse sentido o Brasil conseguiu desenvolver várias atividades importantes, principalmente em relação ao Conhecimento da Biodiversidade, como a Publicação da Flora do Brasil 2020(Brazil Flora Group, 2022). E no âmbito da Amazônia, muitos trabalhos de descrição de novas espécies são evidenciados (Cantuária et al, 2021b;Cantuária et al, 2021c;Cantuária & Silva, 2021;Cantuária et al, 2020;Cantuária et al 2019a;Cantuária et al, 2019b;Krahl et al 2021a;Krahl et al 2021b), não se pode deixar de mencionar que muitos trabalhos oriundos do uso de plantas são conduzidos com o intuito de sistematizar o uso da biodiversidade para alguma atividade biológica (Rodrigues et al, 2022;Rodrigues et al, 2021Jones et al, 2021;Martins et al, 2021). Ou Até mesmo trabalhos que versam sobre o conhecimento de plantas quanto a sua caracterização em grupos com importância taxonômica, ecológica ou econômica (Medeiros et al, 2022;Medeiros et al, 2021;Cantuária et al, 2021a.…”