Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid

Zhang, Can; Wu, Xiaoxiao; Li, Dongyang; Hu, Jinnuo; Wan, Debin; Zhang, Zhen; Hammock, Bruce D.

doi:10.1039/d1ay00129a

Cited by 20 publications

(14 citation statements)

References 37 publications

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“…The visualization results could also be quantitatively analyzed by image software, and the cutoff value was 0.005 ng/mL. For the detection of 3-BPA, the Au@Pt-labeled nanobody lateral-flow nanozyme immunoassay was 20-fold more sensitive than the traditional GNP-Nbs lateral-flow immunoassay . We compare the application of the dot-ELISA, the gold nanoparticle (GNP) lateral-flow immunoassay, and the Au@Pt lateral-flow immunoassay for the detection of 3-PBA.…”

Section: Discussionmentioning

confidence: 99%

“…For the of 3-BPA, the Au@Pt-labeled nanobody lateral-flow nanozyme immunoassay was 20-fold more sensitive than the traditional GNP-Nbs lateral-flow immunoassay. 32 We compare the application of the dot-ELISA, the gold nanoparticle (GNP) lateral-flow immunoassay, and the Au@Pt lateral-flow immunoassay for the detection of 3-PBA. (Table S2 in the Supporting Information).…”

Section: Discussionmentioning

confidence: 99%

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Development of the Au@Pt-Labeled Nanobody Lateral-Flow Nanozyme Immunoassay for Visual Detection of 3-Phenoxybenzoic Acid in Milk and Lake Water

Zhang

et al. 2022

ACS Agric. Sci. Technol.

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As a major metabolite of pyrethroid pesticides, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. A gold–platinum (Au@Pt) nanozyme is a kind of mimic enzyme with unique properties of nanomaterials and catalytic activity. The anti-3-PBA nanobodies were labeled with Au@Pt (Au@Pt-Nbs) by electrostatic adsorption. The Au@Pt-Nbs lateral-flow nanozyme immunoassay was established for the visual and rapid detection of 3-PBA. The visualization results were quantitatively analyzed by Adobe Photoshop CC and got a cutoff value of 0.005 ng/mL. The samples of milk- and lake water-spiked 3-PBA were also analyzed, and the recoveries ranged from 102 to 108% and 95 to 112%, respectively. The results were also validated by liquid chromatography–mass spectrometry and got good correlation (R 2 = 0.945). The Au@Pt-Nbs lateral-flow nanozyme immunoassay was developed by the combination of nanobodies and nanozymes, which will be a promising application in the field of safety inspection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of the Au@Pt-Labeled Nanobody Lateral-Flow Nanozyme Immunoassay for Visual Detection of 3-Phenoxybenzoic Acid in Milk and Lake Water

Zhang

et al. 2022

ACS Agric. Sci. Technol.

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“…This has the potential to reduce cumbersome transport of samples to a laboratory by providing an easily interpretable result in the field. As proof of concept, LFIAs were developed using Nbs targeting aflatoxin B1 in almond milk, and 3-phenoxybenzoic acid in milk and lake water [ 93 , 94 ].…”

Section: Diagnostic Applications Of Nanobodiesmentioning

confidence: 99%

NANOBODIES®: A Review of Diagnostic and Therapeutic Applications

Jin

Odongo

Radwanska

et al. 2023

IJMS

124

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Nanobodies, also referred to as single domain-based VHHs, are antibody fragments derived from heavy-chain only IgG antibodies found in the Camelidae family. Due to their small size, simple structure, high antigen binding affinity, and remarkable stability in extreme conditions, nanobodies possess the potential to overcome several of the limitations of conventional monoclonal antibodies. For many years, nanobodies have been of great interest in a wide variety of research fields, particularly in the diagnosis and treatment of diseases. This culminated in the approval of the world’s first nanobody based drug (Caplacizumab) in 2018 with others following soon thereafter. This review will provide an overview, with examples, of (i) the structure and advantages of nanobodies compared to conventional monoclonal antibodies, (ii) methods used to generate and produce antigen-specific nanobodies, (iii) applications for diagnostics, and (iv) ongoing clinical trials for nanobody therapeutics as well as promising candidates for clinical development.

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“…It made up for the lack of sensitivity caused by urine sample dilution in the conventional method that could achieve an IC 50 value of 0.082 ng mL –1 , a linear range of 0.015–0.447 ng mL –1 , and a LOD of 0.011 ng mL –1 . Based on the same nanobodies, they further developed a rapid flow‐through dot ELISA and AuNPs‐based lateral‐flow immunoassay [ 58 ] for detecting 3‐PBA with high sensitivity and convenient operation.…”

Section: Nanobody‐implemented Immunoassaysmentioning

confidence: 99%

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

Wang

Xianyu

2022

Small Methods

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treatment of cancers, the second leading cause of death in the world that are hard to be treated in the terminal stages. [2] For instance, liver cancer is one of the most lethal cancers and has a poor prognosis with a 5-year survival rate of less than 20%. [3] The early detection and timely treatment of cancers are very important for the patients to save their lives. Microorganism contamination in food is the main cause of foodborne diseases and 70% of patients with foodborne illnesses get sick after taking foods or water contaminated with pathogenic microorganisms. [4] Viruses are one of the major causes of infectious diseases and various public health events like the acquired immunodeficiency syndrome (AIDS), the severe acute respiratory syndrome (SARS), and the coronavirus disease 2019 (COVID-19). [5] Therefore, the timely and rapid detection of microorganisms is of great importance to ensure human health and public safety. [6] In addition, the detections of small molecules including mycotoxins and exogenous pollutions play an important role in food safety. Mycotoxins such as aflatoxin B1, ochratoxin A, and tenuazonic acid are classified as naturally occurring carcinogens by the World Health Organization that are extremely harmful to both humans and animals. [7] They not only directly threaten human life by contaminating food, but also indirectly affect human health through the food chain by contaminating feeds of animals. [8,9] Commonly used immunoassays include enzyme-linked immunosorbent assay (ELISA), quantum dot fluorescence immunoassay, fluorescence polarization immunoassay, colloidal gold immunoassay, chemiluminescent immunoassay (CLIA), electrochemical immunoassay, and microarray-based immunoassay. In spite of the different formats of immunoassays, they share the same principle that relies on the specific binding between antigenic determinants and antibody recognition sites. Therefore, it is of great significance to prepare specific antibodies with high affinity towards the target molecules to improve the specificity of immunoassays. Conventional immunoassays generally use polyclonal and monoclonal antibodies, but both types of antibodies have limitations such as poor specificity, low stability, and complicated preparation. To meet these challenges, a new type of antibody called "nanobody" has emerged. Nanobody is the heavy-chain-only antibody with the advantages of small size, high specificity, mass expression and high stability, which can act as a substitute for conventional antibodies in immunoassays and a promising low-cost immuno-affinity detection reagent.Immunoassay as a rapid and convenient method for detecting a variety of targets has attracted tremendous interest with its high specificity and sensitivity. Among the commonly used immunoassays, enzyme-linked immunosorbent assay has been widely used as a gold standard method in various fields that consists of two main components including a recognition element and an enzyme label. With the rapid advances in nanotechnology, nanobodies and nanozym...

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Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid

Abstract: As a major metabolite of pyrethroids pesticide, 3-phenoxybenzoic acid (3-PBA) can be an indicator of health risk and human exposure assessment. Based on nanobodies (Nbs), we have developed a rapid...

Cited by 20 publications

References 37 publications

Development of the Au@Pt-Labeled Nanobody Lateral-Flow Nanozyme Immunoassay for Visual Detection of 3-Phenoxybenzoic Acid in Milk and Lake Water

Development of the Au@Pt-Labeled Nanobody Lateral-Flow Nanozyme Immunoassay for Visual Detection of 3-Phenoxybenzoic Acid in Milk and Lake Water

NANOBODIES®: A Review of Diagnostic and Therapeutic Applications

Nanobody and Nanozyme‐Enabled Immunoassays with Enhanced Specificity and Sensitivity

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