Development of nNOS-positive neurons in the rat sensory ganglia after capsaicin treatment

Maslyukov, P. M.; Moiseev, K. Yu.; Korzina, M. B.; Porseva, V. V.

doi:10.1016/j.brainres.2015.05.040

Cited by 6 publications

(6 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For all immunohistochemical protocols, at least one section served as a control (did not receive primary or secondary antibodies). In addition, all antibodies used in immunohistochemistry protocols were verified in previously published studies or by the vendor using Western blots (3,16,24,26,39,80,101) or preincubation with immunogen (3,13,17,20,62). Similar to what has been observed previously (20), we found that preincubation with OT immunogen (X50) eliminated OT staining but had no effect on CRFR2 labeling, whereas the blocking peptide for CRFR2 (X50) eliminated nTS CRFR2 staining but had no effect on OT labeling, indicating lack of cross-reactivity.…”

Section: Immunohistochemistrysupporting

confidence: 81%

Hypoxia activates a neuropeptidergic pathway from the paraventricular nucleus of the hypothalamus to the nucleus tractus solitarii

Ruyle

Klutho

Baines

et al. 2018

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

The paraventricular nucleus of the hypothalamus (PVN) contributes to both autonomic and neuroendocrine function. PVN lesion or inhibition blunts cardiorespiratory responses to peripheral chemoreflex activation, suggesting that the PVN is required for full expression of these effects. However, the role of efferent projections to cardiorespiratory nuclei and the neurotransmitters/neuromodulators that are involved is unclear. The PVN sends dense projections to the nucleus tractus solitarii (nTS), a region that displays neuronal activation following hypoxia. We hypothesized that acute hypoxia activates nTS-projecting PVN neurons. Using a combination of retrograde tracing and immunohistochemistry, we determined whether hypoxia activates PVN neurons that project to the nTS and examined the phenotype of these neurons. Conscious rats underwent 2 hr normoxia (21% O, n=5) or hypoxia (10% O, n=6). Hypoxia significantly increased Fos immunoreactivity in nTS-projecting neurons, primarily in the caudal PVN. The majority of activated nTS-projecting neurons contained corticotropin releasing hormone (CRH). In the nTS, fibers expressing the CRH receptor CRFR2 were co-localized with oxytocin fibers and were closely associated with hypoxia-activated nTS neurons. A separate group of animals that received microinjection of AAV2-hSyn-GFP into the PVN exhibited GFP-expressing fibers in the nTS; a proportion of these fibers displayed OT immunoreactivity. Thus, nTS CRFR2s appear to be located on the fibers of PVN OT neurons that project to the nTS. Taken together, our findings suggest that PVN CRH projections to the nTS may modulate nTS neuronal activation, possibly via OTergic mechanisms, and thus contribute to chemoreflex cardiorespiratory responses.

show abstract

Section: Immunohistochemistrysupporting

confidence: 81%

Hypoxia activates a neuropeptidergic pathway from the paraventricular nucleus of the hypothalamus to the nucleus tractus solitarii

Ruyle

Klutho

Baines

et al. 2018

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…Morphine acts on primary nociceptive neurons to activate PI3Kγ/AKT signaling, consequently stimulating the nNOS/NO/K ATP channel to directly block inflammatory hypernociception (Cunha et al, 2010). Upregulated nNOS expression in dorsal root ganglia neurons during nerve injury promotes NO synthesis, and further leads to neuronal excitability, which enhances pain hypersensitivity (Masliukov et al, 2015). The N-methyl-d-aspartate (NMDA) receptor contributes to central nervous system sensitization and chronic pain by activating nNOS (Lee et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Anti-nociceptive effects of Paecilomyces hepiali via multiple pathways in mouse models

Wang

Jia

et al. 2016

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Paecilomyces hepiali (PH), a well-known medicinal fungus, has various pharmacological efficacies. In our study, the antinociceptive effects of PH and underlying mechanisms were evaluated using various mouse models. An acetic acid-induced writhing test, hot plate test, and formalin test were employed to evaluate the antinociceptive activities of PH. The levels of neuronal nitric oxide synthase (nNOS) in the hypothalamus and monoamine neurotransmitters in the serum and hypothalamus of experimental mice were examined. Additionally, hot plate tests using mice pretreated with various antagonists were used to determine the mechanisms of PHmediated antinociception. The PH-enhanced latency period of mice in the hot plate test was significantly blocked by pretreatment with atropine and glibenclamide. PH shortened the phase I and phase II reaction times of formalin-treated mice. Strongly reduced writhing and stretching induced by acetic acid were observed in PH-treated mice, indicating that PH mainly exerts antinociceptive activity on neurogenic pain. After thermal pain stimulation for 30 s, compared to control mice, 7-day PH-treated mice had lower nNOS and dopamine levels, and increased levels of serotonin in both the serum and hypothalamus. Collectively, our data showed that PH mediated antinociceptive activities via multiple pathways, including monoamines, nNOS/ATPsensitive K + channels, and M-type acetylcholine receptors.

show abstract

“…[29] CAP-treatment leads to increased expression and activation of nNOS. [30,31] Numerous studies have suggested that dietary CAP could upregulate the expression of nNOS. [23]…”

Section: Introductionmentioning

confidence: 99%

Capsaicin: A Novel Approach to the Treatment of Functional Dyspepsia

Yuan

Meng

et al. 2023

Molecular Nutrition Food Res

View full text Add to dashboard Cite

Capsaicin, the principal spicy component of red pepper, shows numerous bioactivities these years. Based on the results of past studies, capsaicin may have potential effects on the treatment of functional dyspepsia (FD). However, most studies mainly investigate functional dyspepsia-treatment effects of capsaicin by discussing the relationship between capsaicin and transient receptor potential vanilloid type 1 (TRPV1). In fact, capsaicin may relieve the symptoms of FD in various ways. These effects involve desensitizing C nociceptive fibers, regulating kinds of neurotransmitters, counteracting viruses and inflammation, balancing the gut microbiota, inhibiting the secretion of gastric acid, and reducing oxidative stress damage. A full understanding of these mechanisms will help further development and utilization of capsaicin in food and medical fields.

show abstract

Development of nNOS-positive neurons in the rat sensory ganglia after capsaicin treatment

Cited by 6 publications

References 59 publications

Hypoxia activates a neuropeptidergic pathway from the paraventricular nucleus of the hypothalamus to the nucleus tractus solitarii

Hypoxia activates a neuropeptidergic pathway from the paraventricular nucleus of the hypothalamus to the nucleus tractus solitarii

Anti-nociceptive effects of Paecilomyces hepiali via multiple pathways in mouse models

Capsaicin: A Novel Approach to the Treatment of Functional Dyspepsia

Contact Info

Product

Resources

About