Development of on-line comprehensive two-dimensional liquid chromatography method for the separation of biomass compounds

Masle, Agnès Le; Angot, David; Gouin, Cécile; D’Attoma, Amélie; Ponthus, Jérémie; Quignard, A.; Heinisch, Sabine

doi:10.1016/j.chroma.2014.03.020

Cited by 53 publications

(28 citation statements)

References 26 publications

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“…This work aims at developing an efficient method for the SFC separation of fast pyrolysis bio-oils. The lack of extensive knowledge on the components of such very complex mixtures prevented us from considering model compounds to develop the separation method as it was done in the past for closely related samples [6]. The development of the separation method was therefore carried out on the bio-oil.…”

Section: Methodology For Optimizing Key Sfc Parametersmentioning

confidence: 99%

Development of a supercritical fluid chromatography method with ultraviolet and mass spectrometry detection for the characterization of biomass fast pyrolysis bio oils

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Masle

Charon

et al. 2017

Journal of Chromatography A

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The characterization of complex mixtures is a challenging issue for the development of innovative processes dedicated to biofuels and bio-products production. The huge number of compounds present in biomass fast pyrolysis oils combined with the large diversity of chemical functions represent a bottleneck as regards analytical technique development. For the extensive characterization of complex samples, supercritical fluid chromatography (SFC) can be alternative to usual separation techniques such as gas (GC) or liquid chromatography (LC). In this study, an approach is proposed to define the best conditions for the SFC separation of a fast pyrolysis bio-oil. This approach was based on SFC data obtained directly from the bio-oil itself instead of selecting model compounds as usually done. SFC conditions were optimized by using three specific, easy-to-use and quantitative criteria aiming at maximizing the separation power. Polar stationary phases (ethylpyridine bonded silica) associated to a mix of acetonitrile and water as polarity modifier provided the best results, with more than 120 peaks detected in SFC-UV.

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Section: Methodology For Optimizing Key Sfc Parametersmentioning

confidence: 99%

Development of a supercritical fluid chromatography method with ultraviolet and mass spectrometry detection for the characterization of biomass fast pyrolysis bio oils

Crépier

Masle

Charon

et al. 2017

Journal of Chromatography A

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“…Le Masle et al [58] used on-line RPLC x RPLC for the characterization of bio-oils obtained by fast pyrolysis of lignocellulosic biomass (mainly neutral and acidic compounds). Accurate knowledge of biooil composition is essential for optimizing the process of upgrading bio-oil to biofuel.…”

Section: Lc X Uhplc In Bioenergymentioning

confidence: 99%

“…RPLC × RPLC separation of a partially dehydroxygenated bio-oil using the coupling of Hypercarb (100 × 1 mm, 5 μm) in 1 D and Acquity CSH phenyl-Hexyl (50 × 2.1 mm, 1.7 μm) in 2 D. {Adapted from[58]}.…”

mentioning

confidence: 99%

Theoretical and practical interest in UHPLC technology for 2D-LC

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Crétier

Heinisch

2014

TrAC Trends in Analytical Chemistry

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“…Two-dimensional reversed phase liquid chromatography (RPLC) techniques were applied to the analysis of the aqueous fraction of a bio-oil. It was found that RPLC x RPLC had the potential of resolving up to 2000 peaks [7], highlighting the potential of this technique for the comprehensive analysis of the aqueous phase of the bio-oil. Both photo diode array (PDA) and MS detection were latter coupled to RPLC x RPLC and a more detailed analysis could be obtained [8].…”

Section: Introductionmentioning

confidence: 99%

Ultra-high performance supercritical fluid chromatography hyphenated to atmospheric pressure chemical ionization high resolution mass spectrometry for the characterization of fast pyrolysis bio-oils

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Masle

Charon

et al. 2018

Journal of Chromatography B

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Extensive characterization of complex mixtures requires the combination of powerful analytical techniques. A Supercritical Fluid Chromatography (SFC) method was previously developed, for the specific case of fast pyrolysis bio oils, as an alternative to gas chromatography (GC and GC × GC) or liquid chromatography (LC and LC × LC), both separation methods being generally used prior to mass spectrometry (MS) for the characterization of such complex matrices. In this study we investigated the potential of SFC hyphenated to high resolution mass spectrometry (SFC-HRMS) for this characterization using Negative ion Atmospheric Pressure Chemical ionization ((-)APCI) for the ionization source. The interface between SFC and (-)APCI/HRMS was optimized from a mix of model compounds with the objective of maximizing the signal to noise ratio. The main studied parameters included both make-up flow-rate and make-up composition. A methodology for the treatment of APCI/HRMS data is proposed. This latter allowed for the identification of molecular formulae. Both SFC-APCI/HRMS method and data processing method were applied to a mixture of 36 model compounds, first analyzed alone and then spiked in a bio-oil. In both cases, 19 compounds could be detected. Among them 9 could be detected in a fast pyrolysis bio-oil by targeted analysis. The whole procedure was applied to the characterization of a bio-oil using helpful representations such as mass-plots, van Krevelen diagrams and heteroatom class distributions. Finally the results were compared with those obtained with a Fourier Transform ion-cyclotron resonance mass spectrometer (FT-ICR/MS).

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Development of on-line comprehensive two-dimensional liquid chromatography method for the separation of biomass compounds

Cited by 53 publications

References 26 publications

Development of a supercritical fluid chromatography method with ultraviolet and mass spectrometry detection for the characterization of biomass fast pyrolysis bio oils

Development of a supercritical fluid chromatography method with ultraviolet and mass spectrometry detection for the characterization of biomass fast pyrolysis bio oils

Theoretical and practical interest in UHPLC technology for 2D-LC

Ultra-high performance supercritical fluid chromatography hyphenated to atmospheric pressure chemical ionization high resolution mass spectrometry for the characterization of fast pyrolysis bio-oils

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