Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent

Kurosumi, Akihiro; Kobayasi, Fumihisa; Mtui, Godliving Y. S.; Nakamura, Yoshitoshi

doi:10.1016/j.bej.2006.02.004

Cited by 22 publications

(9 citation statements)

References 12 publications

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“…Also, the production of organic acids and secondary metabolites such as enzymes might inhibit the mycelial growth due to endproduct inhibition. 1 Especially, bacteriolysis might be occurred at an initial glucose concentration 50 g/L, so that the DCW of 50 g/L (3.61 g/L) was lower than that of 20 g/L (3.99 g/L). Therefore, it was found that the best carbon source was glucose and the initial glucose concentration should be 20 g/L in order to obtain a large amount of mycelia at a shorter incubation time.…”

Section: Effect Of Carbon Source On Fungal Growthmentioning

confidence: 99%

“…Lentinum from Lentinus edodes and Sonifilan from Schizophyllum commune, are well-known and appear to have strong anticancer activities. [1][2][3] Yamabushitake, or Hericium erinaceum, is an edible mushroom fungus belonging to Aphyllophorales, Hydnaceae (Hericiaceae), Hericium, grows widely in Japan and the whole of Chine. Compounds isolated from H. erinaceum have shown interesting biological activities such as cytotoxic effects on HeLa cells, promotion of the synthesis of nerve growth factor (NGF), and inhibition of pollen germination.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the possibility of environmental pollution due to high level of biochemical oxygen demand (BOD), effective utilization of whey is anticipated. 1 In this work, the optimal carbon source to obtain a large amount of H. erinaceum mycelia was investigated. Furthermore, we used yoghurt whey as an alternative nitrogen source in submerged cultivation of H. erinaceum mycelia, and then compared with chemical nutrient medium.…”

Section: Introductionmentioning

confidence: 99%

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EXAMINATION OF INCUBATION CONDITIONS FOR PRODUCTION OF HERICIUM ERINACEUM

Okumura

Sasaki

Asada

et al. 2012

Int. J. Mod. Phys. Conf. Ser.

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Basidiomycetes has recently attracted considerable attention for its various physiological activities, such as antitumor, antioxidant and immunostimulating activities. Compounds isolated from fruit body of Hericium erinaceum, commonly called Yamabushitake in Japan, have interesting biological activities such as cytotoxic effectors on cancer cell (HeLa cells) and stimulators of synthesis of nerve growth factor. It is necessary for the cultivation of the fruit body of mushroom to control light, temperature, humidity. Otherwise, mycelia cultivation needs only temperature control. H. erinaceum cultivated by submerged culture have similar physiological activities to the fruit body of H. erinaceum, which suggests cultured mycelia can potentially become a promoter of synthesis of nerve growth factor.In this study, we used whey which is by-products of cheese-making process as an alternative nitrogen source in submerged cultivation of H. erinaceum mycelia, and then dry cell weight (DCW) and DCW productivity of whey medium were compared with those of chemical nutrient medium. When whey was used as a nitrogen source, DCW and DCW productivity are 1.5 times higher than those of chemical nutrient medium, 5.99 g/L and 0.60 g/L/day, respectively. It was suggested that whey could be used as an alternative nitrogen source and a growth promoting factor in H. erinaceum mycelia cultivation.

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Section: Effect Of Carbon Source On Fungal Growthmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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EXAMINATION OF INCUBATION CONDITIONS FOR PRODUCTION OF HERICIUM ERINACEUM

Okumura

Sasaki

Asada

et al. 2012

Int. J. Mod. Phys. Conf. Ser.

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“…isolate has been achieved in batch cultures (Muruke et al, 2006). In addition, -1,3-glucan, an antineoplastic (antitumor or cytotoxic compound) has been produced from a basidiomycete Sparassis crispa in shake-flask liquid cultures (Kurosumi et al, 2006). The results of Mtui 2863 these bioconversion reports suggest that waste biomass are potential substrates for production of value-added compounds of economic importance.…”

Section: Production Of Organic Acids and Other Compounds Of Economic mentioning

confidence: 99%

Trends in industrial and environmental biotechnology research in Tanzania

Mtui¹

2007

Afr. J. Biotechnol.

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This paper reviews the trends in industrial and environmental biotechnology research in Tanzania for the past 20 years. Researches on production of bio-energy, enzymes and organic acids are reviewed. Furthermore, researches related to wastewater treatment systems including water stabilization ponds and constructed wetlands are covered. Brief highlights are made on researches related to bioprospecting and molecular biology techniques used in identification of organisms. In critically reviewing the research done so far, an attempt is made to pinpoint significant research gaps in areas such as single cell protein, biomining, biocomposting and bioengineering. This paper, therefore, provides an overview of the renewable raw materials available in the developing countries and outlines the researches that have been done to convert them to bio-products, while reducing bio-wastes. The identified gaps will serve as guidance to scientists who are interested in doing research in untapped areas of biotechnology.

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“…Basidiomycetes are classified as fungi, and their edible fruiting bodies are known as mushrooms. In recent years, various biological and pharmacological activities of certain types of mushrooms have been revealed; for example, lentinan from Lentinus edodes and sonifilan from Schizophyllum commune are well known as anti-tumor polysaccharides [1,2]. Yamabushitake, or Hericium erinaceum, is an edible mushroom that belongs to the Aphyllophorales order and Hydnaceae (Hericiaceae) class.…”

Section: Introductionmentioning

confidence: 99%

Acceleration of Hericium erinaceum mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source

Asada¹,

Okumura²,

Sasaki³

et al. 2012

ABB

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The effects of various carbon sources and their initial concentrations on mycelia production by Hericium erinaceum were investigated by determining the dry cell weight (DCW) and β-glucan content of mycelia in submerged culture. Glucose and xylose were superior carbon sources for promoting mycelial growth resulting in mycelial concentrations of 3.99 g/L and 4.01 g/L, respectively; glucose was the best carbon source in terms of productivity (0.44 g/L/day). Experiments were also performed using yogurt whey as an alternative nitrogen source for submerged cultivation of H. erinaceum mycelia, and DCW and β-glucan content were compared with those with chemical nutrient medium. When whey was used as a nitrogen source, DCW and total amount of β-glucan were 2.3- and 2.8-fold higher, respectively, than that with chemical nutrient medium. Thus, whey appears to be an alternative nitrogen source for promoting H. erinaceum mycelial growth

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Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent

Cited by 22 publications

References 12 publications

EXAMINATION OF INCUBATION CONDITIONS FOR PRODUCTION OF HERICIUM ERINACEUM

EXAMINATION OF INCUBATION CONDITIONS FOR PRODUCTION OF HERICIUM ERINACEUM

Trends in industrial and environmental biotechnology research in Tanzania

Acceleration of <i>Hericium erinaceum</i> mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source

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Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent

Cited by 22 publications

References 12 publications

EXAMINATION OF INCUBATION CONDITIONS FOR PRODUCTION OF HERICIUM ERINACEUM

EXAMINATION OF INCUBATION CONDITIONS FOR PRODUCTION OF HERICIUM ERINACEUM

Trends in industrial and environmental biotechnology research in Tanzania

Acceleration of &lt;i&gt;Hericium erinaceum&lt;/i&gt; mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source

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Acceleration of <i>Hericium erinaceum</i> mycelial growth in submerged culture using yogurt whey as an alternative nitrogen source