Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine

Lee, S.; Lee, G.; Choi, In-Cheol; Rho, Jae-Young

doi:10.1007/s12088-015-0518-8

Cited by 7 publications

(4 citation statements)

References 14 publications

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“…Nested PCR successfully detected a number of important viruses on olive plant cutting which was initially difficult to do using conventional PCR method (Pantaleo et al, 2001). Nested PCR was also used regularly by South Korea's quarantine agency to detect Tobacco ringspot virus (TRSV) on various plants that propagated by seed (Lee et al, 2015). Based on the visual symptom on the indicator plant, the particle morphology and PCR analysis proved that the mosaic and dwarf on petunia was infected by Tobamovirus.…”

Section: Resultsmentioning

confidence: 99%

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

Astuti¹,

Sulandari²,

Hartono³

et al. 2021

Jurnal Hama dan Penyakit Tumbuhan Tropika

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Yellow mosaic stunt disease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. The disease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of the disease may be carried over to imported seeds and necessary to identify as a basic information for developing control strategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents was conducted using electron microscope with quick dipping method and the molecular detection was done using nested PCR with TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculation showed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and vein banding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sized approximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis, the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, a plant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the first report of ReMV in Indonesia infecting Petunia as ornamental plant.

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Section: Resultsmentioning

confidence: 99%

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

Astuti¹,

Sulandari²,

Hartono³

et al. 2021

Jurnal Hama dan Penyakit Tumbuhan Tropika

View full text Add to dashboard Cite

show abstract

“…Nested PCR was also used regularly by South Korea's quarantine agency to detect Tobacco ringspot virus (TRSV) on various plants that propagated by seed (Lee et al, 2015). Based on the visual symptom on the indicator plant, the particle morphology and PCR analysis proved that the mosaic and dwarf on petunia was infected by Tobamovirus.…”

Section: Resultsmentioning

confidence: 99%

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

Astuti

Sulandari

Hartono

et al. 2021

JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Detection and identification of yellow mosaic stunt disease on Petunia sp. using nested PCR method. Yellow mosaic stuntdisease was found at some nurseries of Petunia in Sleman, Yogyakarta, also in Muntilan and Magelang Central Java. Thedisease was very important due to its ability reducing the quality and quantity of Petunia seedlings. The causal agent of thedisease may be carried over to imported seeds and necessary to identify as a basic information for developing controlstrategies. This research was done by mechanical transmission on indicator plants. The observation of the causal agents wasconducted using electron microscope with quick dipping method and the molecular detection was done using nested PCRwith TobRT up1-TobRT do2 as the external primers and TobN up3-TobN do4 as the internal primers. Mechanical inoculationshowed chlorosis symptoms that developed into local spot on Chenopodium amaranticolor as well as mosaic and veinbanding on Nicotiana benthamiana. The observation using electron microscope showed rod-shaped virus particles sizedapproximately 300 nm and by PCR method produced around 568 bp and 400 bp DNA band. Based on the sequence analysis,the disease was caused by Rehmania mosaic virus. This type of Tobamovirus has 96% similarity with ReMV-Japan. ReMV, aplant pathogen which was a member of Tobamovirus that has never been reported in Indonesia. This research was the firstreport of ReMV in Indonesia infecting Petunia as ornamental plant.

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“…Habran A, Commisso M, Helwi P, Hilbert G, Negri S, Ollat N, Gomes E, van Leeuwen C, Guzzo F, Delrot S (2016) Roostocks/scion/nitrogen interactions affect secondary metabolism in the grape berry. Front Plant Sci 7:1134 Hammer GL, Chapman S, Van Oosterom E, Podlich DW (2005) Martin and Converse (1982), Converse (1987), Tzanetakis (2010), Petrzik et al (1998, Thompson and Jelkman (2003), , Tzanetakis et al (2007), Yoshikawa et al (1986), Yoshikawa and Inouye (1988) (2002), Lee et al (2015), Bargen et al (2015), Weelink et al (2000), Loudes et al (1995) Whiteflies Beet Pseudo-Yellows Virus (Bpyv) Strawberry Pallidosis Associated Virus (Spav) Tzanetakis et al (2003Tzanetakis et al ( , 2006, , Wintermantel et al (2006), Ragab et al (2009), Constable et al (2010) Rubus spp. et al (2000), Zalloua et al (1996), Rott et al (1991Rott et al ( , 1995 Thrips 1998), , Susaimuthu et al (2007)…”

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Breeding Climate-Resilient Bananas

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2020

Genomic Designing of Climate-Smart Fruit Crops

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Development of PCR Diagnostic System for Detection of the Seed-Transmitted Tobacco Ringspot Virus in Quarantine

Cited by 7 publications

References 14 publications

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

DETECTION AND IDENTIFICATION OF YELLOW MOSAIC STUNT DISEASE ON Petunia sp. USING NESTED PCR METHOD

Breeding Climate-Resilient Bananas

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