Abstract:Dengue virus (DENV) nonstructural 1 (NS1) protein is a specific and sensitive biomarker for the diagnosis of dengue. In this study, an efficient electrochemical biosensor that uses chemically modified affinity peptides was developed for the detection of dengue virus NS1. A series of amino acid-substituted synthetic peptides was rationally designed, chemically synthesized and covalently immobilized to a gold sensor surface. The sensor performance was monitored via square wave voltammetry (SWV) and electrochemic… Show more
“…The rapid detection (90 min), low resource setting, and sensitivity favours scalability of the platform. In addition to aptamers, chemically-modified peptides are also known to be used as molecular receptor in electrochemical biosensor ( J. H. Kim et al, 2019 ). Figure 7 A) Illustration of non-optical multiplex PCR system (consisting a washing buffer component, microfluidic platform, and a paper based nucleic acid sensor) developed for DENV diagnostics.…”
Section: Iomt-assisted Poc Biosensing For Infectious Diseasesmentioning
On global scale, the current situation of pandemic is symptomatic of increased incidences of contagious diseases caused by pathogens. The faster spread of these diseases, in a moderately short timeframe, is threatening the overall population wellbeing and conceivably the economy. The inadequacy of conventional diagnostic tools in terms of time consuming and complex laboratory-based diagnosis process is a major challenge to medical care. In present era, the development of point-of-care testing (POCT) is in demand for fast detection of infectious diseases along with “on-site” results that are helpful in timely and early action for better treatment. In addition, POCT devices also play a crucial role in preventing the transmission of infectious diseases by offering real-time testing and lab quality microbial diagnosis within minutes. Timely diagnosis and further treatment optimization facilitate the containment of outbreaks of infectious diseases. Presently, efforts are being made to support such POCT by the technological development in the field of internet of medical things (IoMT). The IoMT offers wireless-based operation and connectivity of POCT devices with health expert and medical centre. In this review, the recently developed POC diagnostics integrated or future possibilities of integration with IoMT are discussed with focus on emerging and re-emerging infectious diseases like malaria, dengue fever, influenza A (H1N1), human papilloma virus (HPV), Ebola virus disease (EVD), Zika virus (ZIKV), and coronavirus (COVID-19). The IoMT-assisted POCT systems are capable enough to fill the gap between bioinformatics generation, big rapid analytics, and clinical validation. An optimized IoMT-assisted POCT will be useful in understanding the diseases progression, treatment decision, and evaluation of efficacy of prescribed therapy.
“…The rapid detection (90 min), low resource setting, and sensitivity favours scalability of the platform. In addition to aptamers, chemically-modified peptides are also known to be used as molecular receptor in electrochemical biosensor ( J. H. Kim et al, 2019 ). Figure 7 A) Illustration of non-optical multiplex PCR system (consisting a washing buffer component, microfluidic platform, and a paper based nucleic acid sensor) developed for DENV diagnostics.…”
Section: Iomt-assisted Poc Biosensing For Infectious Diseasesmentioning
On global scale, the current situation of pandemic is symptomatic of increased incidences of contagious diseases caused by pathogens. The faster spread of these diseases, in a moderately short timeframe, is threatening the overall population wellbeing and conceivably the economy. The inadequacy of conventional diagnostic tools in terms of time consuming and complex laboratory-based diagnosis process is a major challenge to medical care. In present era, the development of point-of-care testing (POCT) is in demand for fast detection of infectious diseases along with “on-site” results that are helpful in timely and early action for better treatment. In addition, POCT devices also play a crucial role in preventing the transmission of infectious diseases by offering real-time testing and lab quality microbial diagnosis within minutes. Timely diagnosis and further treatment optimization facilitate the containment of outbreaks of infectious diseases. Presently, efforts are being made to support such POCT by the technological development in the field of internet of medical things (IoMT). The IoMT offers wireless-based operation and connectivity of POCT devices with health expert and medical centre. In this review, the recently developed POC diagnostics integrated or future possibilities of integration with IoMT are discussed with focus on emerging and re-emerging infectious diseases like malaria, dengue fever, influenza A (H1N1), human papilloma virus (HPV), Ebola virus disease (EVD), Zika virus (ZIKV), and coronavirus (COVID-19). The IoMT-assisted POCT systems are capable enough to fill the gap between bioinformatics generation, big rapid analytics, and clinical validation. An optimized IoMT-assisted POCT will be useful in understanding the diseases progression, treatment decision, and evaluation of efficacy of prescribed therapy.
“…Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square-wave voltammetry (SWV) are most popular voltammetric techniques that allow for the determination of low concentrations of flaviviruses, which is attractive for biomedical and biological research [66][67][68]. These methods are successfully used in the creation of label-free biosensors for the determination of viral analytes.…”
Section: Voltammetry Amperometrymentioning
confidence: 99%
“…Peptides can easily bind to the electrode surfaces of various materials, such as metal nanoparticles (NPs) or carbon nanotubes (CNTs), for bioassays. Recently, an effective label-free electrochemical immunosensors based on binding affinities of five synthetic affinity peptides for the detection of non-structural protein NS1 (Figure 4) were developed [68]. Relative binding affinities of these synthetic peptides were determined by SWV and EIS.…”
Section: Peptide-based Biosensors For Denv Diagnosticmentioning
confidence: 99%
“…Biosensor chips were developed for the label-free detection of ZIKV NS1 antigen in a simulated human serum, with LOD of 4.5 × 10 −10 mol/L. Similar works were carried out for determination of the DENV and described in chapter [68,88].…”
Section: Electrochemical Biosensors For Zikv Diagnosticmentioning
A highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. Among a wide range of viral pathogens, Flaviviruses attract a special attention. Flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV). Haemorrhagic and encephalitis diseases are the most common severe consequences of flaviviral infection. Currently, increasing attention is being paid to the development of electrochemical immunological methods for the determination of Flaviviruses. This review critically compares and evaluates recent research progress in electrochemical biosensing of DENV, ZIKV, and JEV without labelling. Specific attention is paid to comparison of detection strategies, electrode materials, and analytical characteristics. The potential of so far developed biosensors is discussed together with an outlook for further development in this field.
“…Another approach uses peptide-display phages as an alternative for the detection of viral diseases 19 . Peptide-display phages present some advantages: can be easily synthesised and their use can reduce the complexity of the development of platforms such as lateral ow assays or biosensor approaches, due to the ease coupled to nanoparticles or biomolecules 20,21 . The improvement of the DENV detection test, especially in the early stage of illness, it is essential to generate peptides or antibodies with no cross-reaction with other arboviruses.…”
Dengue is a viral disease caused by any of the four distinct dengue virus (DENV) serotypes that circulate in many parts of the world. DENV now co-circulates with Zika and Chikungunya viruses (ZIKV and CHIKV) in many regions of the Americas. Having this in mind, plus the fact that DENV clinical diagnosis persists as a difficult task, due to the similarity in symptoms, as well as false-positive results by the cross-reactivity of the IgG and IgM against these three viruses, correct identification of DENV at an early stage of the disease is essential to minimise transmission and prevent potentially devastating sequelae. Here, by phage display, we isolate specific peptides for dengue virus NS1 protein. The specificity of the linear peptides as diagnostic tools for DENV NS1 protein in sera samples was investigated, and the selected peptides showed the ability to recognise DENV, and no cross-reactivity was shown.
Moreover, in silico analysis was performed to assess the possible binding modes of these peptides to DENV-NS1, using a molecular docking approach. These peptides are suitable for use in an ELISA assay for dengue virus detection in human serum and the possibility to adapt these peptides to PoC platforms.
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