Development of portable electronic reader for picoampere detection for two-electrode based amperometric biosensor applications

Rajapaksha, R. D. A. A.; Yahaya, Nurshieren; Uda, M. N. A.; Hashim, U.

doi:10.1063/1.5080834

Cited by 6 publications

(2 citation statements)

References 12 publications

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“…This effort aligns with the changing landscape of medical research, where fields like biotechnology and electronics are converging to develop state-of-the-art solutions [5], [6]. Consequently, a new approach has emerged electrical-based biosensors for detecting COVID-19 [7]- [9].…”

Section: Introductionmentioning

confidence: 93%

Advancing COVID-19 Detection: High-Performance RNA Biosensing via Electrical Interactions

Uda Hashim,

M. N. Afnan Uda,

M. N. A. Uda

et al. 2024

IJNeaM

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This research paper investigated the detection of COVID-19 using an Aluminum Interdigitated Electrode (Al-IDE) sensor based on electrical conductivity. The silanization process involved the functionalization step, employing (3-Aminopropyl) triethoxysilane (APTES), while the immobilization process was facilitated by the RNA Probe specific to COVID-19. To verify its specificity in detection, the functionalized biosensor was tested against single-base mismatches, non-complementary sequences, and complementary sequences. The physical characteristics of the Al-IDE biosensor were examined using both low-power microscopy (LPM) and high-power microscopy (HPM). Additionally, the morphological properties of the biosensor were assessed using atomic force microscopy (AFM). To assess its diagnostic potential, the biosensor's sensitivity was evaluated by exposing it to a range of complementary targets, spanning from 1 femtomolar (fM) to 1 micromolar (μM). The current-voltage (I-V) characteristics of the biosensor were meticulously analyzed at each stage of functionalization bare Al-IDE, silanization, immobilization, and hybridization. This I-V characterization was carried out using a picoammeter voltage source (Keithley 2450), Kickstart software, and a probe station. The results confirmed the biosensor's capability to effectively detect COVID-19 targets within the nanoampere concentration range, demonstrating its success in detecting specific COVID-19 targets at the nanoampere level.

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Section: Introductionmentioning

confidence: 93%

Advancing COVID-19 Detection: High-Performance RNA Biosensing via Electrical Interactions

Uda Hashim,

M. N. Afnan Uda,

M. N. A. Uda

et al. 2024

IJNeaM

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“…In several domains, such as industrial process control, biological diagnostics, and environmental monitoring, electrochemical sensing has become an indispensable instrument [21]- [23]. Interdigitated electrodes (IDEs), one of the many types of electrodes used in electrochemical sensing, have attracted a lot of interest because of its low cost, high sensitivity, and ability to be miniaturised.…”

Section: Introductionmentioning

confidence: 99%

Aluminium Interdigitated Electrode with 5.0 μm Gap for Electrolytic Scooting

M. N. Afnan Uda,

Uda Hashim,

M. N. A. Uda

et al. 2024

IJNeaM

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The goal of the research project is to design, fabricate, and characterize an extremely sensitive biosensor for use in healthcare. Using AutoCAD software, a novel IDE pattern with a 5 μm finger gap was created. Conventional photolithography and regular CMOS technology were used in the fabrication process. A 3D nano profiler, scanning electron microscopy (SEM), high-power microscopy (HPM), and low-power microscopy (LPM) were used to physically characterize the manufactured IDE. Chemical testing was done using several pH buffer solutions, and electrical validation was performed using I-V measurements. The Al IDE was produced, with a tolerance of 0.1 μm between the fabricated IDEs and the design mask. Electrical measurements verified the flawless fabrication of the IDE, and the device's repeatability was validated by the outcomes of comparable IDE samples. For each pH buffer solution, a modest additional volume of 2 μl was used to quantitatively detect slight current fluctuations in the microampere range. Through pH calibration for advanced applications in the realm of chemical sensors using an amperometric method, this research study has verified the chemical behavior of the IDE.

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