2019
DOI: 10.1111/jam.13938
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Development of probe‐based real‐time loop‐mediated isothermal amplification for detection ofBrucella

Abstract: The developed probe based RT-LAMP is a powerful gene amplification technique is a specific, fast diagnostic tool for early detection and identification of Brucella. This article is protected by copyright. All rights reserved.

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Cited by 14 publications
(9 citation statements)
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“…Molecular biological detection of Brucella is abundant [9,20,21]. However, there were a few PCR methods for detecting canine Brucella [10,11].…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Molecular biological detection of Brucella is abundant [9,20,21]. However, there were a few PCR methods for detecting canine Brucella [10,11].…”
Section: Discussionmentioning
confidence: 99%
“…Its effectiveness is affected by the infected animal's bacteremia level [7]. Molecular biological detection technology has the characteristics of safety and reliability, high sensitivity, strong speci city and simple operation, and has begun to be popularized and applied to detect Brucella [8,9]. Now there were a variety of PCR methods for detecting canine Brucella [10,11], such as bruce-ladder [11] and multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) procedures [12].…”
Section: Introductionmentioning
confidence: 99%
“…27,68 DARQ LAMP was also applied to detect Salmonella, 69 avian reovirus, 70 methicillin-resistant Staphylococcus aureus (MRSA) 71 and Brucella. 72 Analytical sensitivity for Salmonella DARQ LAMP was 10 copies per reaction. High specicity against non-Salmonella strains was shown.…”
Section: Alternately Binding Quenching Probe Competitive Lamp (Abc-lamp)mentioning
confidence: 99%
“…In this study, we developed a multiplex SFTSV/OT/IC loop‑mediated isothermal amplification (LAMP) assay for the differential diagnosis of SFTSV and O . tsutsugamushi infection, based on a slightly modified DARQ probe method [ 33 , 34 ]. The detection limit of the multiplex SFTSV/OT/IC RT-LAMP assay was confirmed using diluted SFTS and scrub typhus clinical samples.…”
Section: Introductionmentioning
confidence: 99%