Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs

McLauchlan, A; Ogbonnaya, Francis C.; Hollingsworth, Brandon; Carter, M; Gale, K. R.; Henry, Robert J; Holton, Timothy A; Morell, Michael K; Rampling, Lynette; Sharp, P. J.; Shariflou, M. R.; Jones, Michael G. K.; Appels, R.

doi:10.1071/ar01036

Cited by 57 publications

(27 citation statements)

References 16 publications

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“…McLauchlan et al (2001) applied waxy markers to detect null alleles in Australian breeding programme. Shan et al (2007) and Liu et al (2008) successfully used these markers to screen their materials for the presence of null alleles.…”

Section: Molecular Characterisation Of the Allelic Variationmentioning

confidence: 99%

Wheat waxy proteins: polymorphism, molecular characterization and effects on starch properties

Guzmán

Álvarez

2015

Theor Appl Genet

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The starch fraction, comprising about 70% of the total dry matter in the wheat grain, can greatly affect the end-use quality of products made from wheat kernels, especially Asian noodles. Starch is associated with the shelf life and nutritional value (glycaemic index) of different wheat products. Starch quality is closely associated with the ratio of amylose to amylopectin, the two main macromolecules forming starch. In this review, we briefly summarise the discovery of waxy proteins-shown to be the sole enzymes responsible for amylose synthesis in wheat. The review particularly focuses on the different variants of these proteins, together with their molecular characterisation and evaluation of their effects on starch composition. There have been 19 different waxy protein variants described using protein electrophoresis; and at a molecular level 19, 15 and seven alleles described for Wx-A1, Wx-B1 and Wx-D1, respectively. This large variability, found in modern wheat and genetic resources such as wheat ancestors and wild relatives, is in some cases not properly ordered. The proper ordering of all the data generated is the key to enhancing use in breeding programmes of the current variability described, and thus generating wheat with novel starch properties to satisfy the demand of industry and consumers for novel high-quality processed food.

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Section: Molecular Characterisation Of the Allelic Variationmentioning

confidence: 99%

Wheat waxy proteins: polymorphism, molecular characterization and effects on starch properties

Guzmán

Álvarez

2015

Theor Appl Genet

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“…Mutations in any one of these genes will result in the nonexpression of that GBSS protein, reducing starch amylose content . Loss of all three proteins results in 'waxy' endosperm, or virtually no amylose deposited in the starch granules (McLauchlan et al 2001). All of the above-mentioned genes have specific impacts on end-use quality performance and are major genetic improvement targets for wheat breeding programs around the globe.…”

Section: Introductionmentioning

confidence: 99%

Chromosomes 3B and 4D are associated with several milling and baking quality traits in a soft white spring wheat (Triticum aestivum L.) population

et al. 2011

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Wheat is marketed based on end-use quality characteristics and better knowledge of the underlying genetics of specific quality parameters is essential to enhance the breeding process. A set of 188 recombinant inbred lines from a 'Louise' by 'Penawawa' mapping population was grown in two crop years at two locations in the Pacific Northwest region of the United States and data were collected on 17 end-use quality traits using established quality analysis protocols. Using an established genetic linkage map, composite interval mapping was used to identify QTL associated with 16 of the 17 quality traits. QTL were found on 13 of the 21 wheat chromosomes. A large number of QTL were located on chromosomes 3B and 4D and coincided with traits for milling quality and starch functionality. Chromosome 3B contained 10 QTL, which were localized to a 26.2 cM region. Chromosome 4D contained 7 QTL, all of which were located on an 18.8 cM region of this chromosome. The majority of the alleles for superior end-use quality were associated with the cultivar Louise. The identified QTL detected remained highly significant independent of grain yield and protein quantity. The identification of these QTL for end-use quality gives key insight into the relationship and complexity of end-use quality traits. It also improves our understanding of these relationships, thereby allowing plant breeders to make valuable gains from selection for these important traits.

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“…Furthermore, CIMMYT has validated other markers for a gene that confers resistance to Hessian fly and two others for Russian wheat aphid (http://www.maswheat.ucdavis.edu) which are important for CIMMYT's national program partners. Gene profiling of parental materials has also allowed desirable quality traits such as the Glu1Bx over-expression, known to be associated with increased dough strength (Juhasz et al 2003), the granule bound starch synthase null allele (GBSS: Mclauchlan et al 2001), which increases swelling volume, and desired combinations of grain hardness alleles that are associated with milling yield Morris 1997, 1998).…”

Section: Cimmyt's Experiencementioning

confidence: 98%

Wheat breeding assisted by markers: CIMMYT’s experience

2007

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Significant progress has been made in the characterization of loci controlling traits of importance using molecular markers. A number of markers are currently available in wheat for genes of interest to the breeders. Markers can be used to better characterize parental material, thereby improving the efficiency and effectiveness of parental selection for crossing and to track genes in segregating progenies through the selection process. Although a number of breeding programs are using molecular markers at modest levels, the costs associated with marker assisted selection (MAS) are frequently cited as the main constraint to their wide-spread use by plant breeders. However, this is likely to change when user-friendly, high-throughput, automated marker technologies based on single nucleotide polymorphisms become available. These evolving technologies will increase the number of available markers, and will improve the efficiency, throughput, and cost effectiveness of MAS, thereby making it more attractive and affordable to many breeding programs. This article examines the extent to which molecular markers have been used at the International Maize and Wheat Improvement Center (CIMMYT) in applied wheat breeding and reviews the limited publicly available information on MAS from other wheat breeding programs. As markers are currently available for relatively few traits, we believe that MAS must be integrated with ongoing conventional breeding to maximize its impact. When used in tandem with phenotypic selection, MAS will improve response to selection for certain traits, thereby increasing rates of genetic progress.

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Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs

Cited by 57 publications

References 16 publications

Wheat waxy proteins: polymorphism, molecular characterization and effects on starch properties

Wheat waxy proteins: polymorphism, molecular characterization and effects on starch properties

Chromosomes 3B and 4D are associated with several milling and baking quality traits in a soft white spring wheat (Triticum aestivum L.) population

Wheat breeding assisted by markers: CIMMYT’s experience

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