Development of screen-printed enzyme electrodes for the estimation of fish quality

Chemnitius, Gabriele-Christine; Bilitewski, Ursula

doi:10.1016/0925-4005(96)80118-3

Cited by 52 publications

(34 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although feasible, the electrochemical measurement of H 2 O 2 with a Pt electrode [3,5,8] requires a high potential (600 -700 mV) and is susceptible to endogenous interferences. The interaction and electron exchange between redoxenzymes and an electrode interface has attracted increasing attention [11 -13].…”

Section: Introductionmentioning

confidence: 99%

Multiwall Carbon Nanotube (MWCNT) Based Electrochemical Biosensors for Mediatorless Detection of Putrescine

Luong

Hrapovic

Wang³

2005

Electroanalysis

View full text Add to dashboard Cite

g-Aminopropyltriethoxysilane (APTES)-induced solubilization of multi-wall carbon nanotube CNTs allowed for the modification of electrode surfaces. APTES also served as an immobilization matrix for putrescine oxidase (POx) to construct an amperometric biosensor. Although CNTs modified by APTES acted as semiconductors to reduce the exposed sensing surface, we reasoned that nanoscale "dendrites" of CNTS modified by APTES formed a network and projected outwards from the electrode surface and acted like bundled ultra-microelectrodes that allowed access to the active site and facilitated direct electron transfer to the immobilized enzyme. Our biosensor was able to efficiently monitor direct electroactivity of POx at the electrode surface. The putrescine biosensor prepared using the modified glassy carbon electrode exhibited current response within 10 s with a detection limit of 500 nM.

show abstract

Section: Introductionmentioning

confidence: 99%

Multiwall Carbon Nanotube (MWCNT) Based Electrochemical Biosensors for Mediatorless Detection of Putrescine

Luong

Hrapovic

Wang³

2005

Electroanalysis

View full text Add to dashboard Cite

show abstract

“…These values are in good agreement to those found for other fish samples. 37,38 The progress of spoilage found and the increase Fig. 4 Fluorescence signal in a sensing plate with DEA, TEA, cysteine and ammonia at concentrations of 0.1 mg mL À1 and 1 mg mL À1 against a histamine concentration of 50 mg mL À1 at different time intervals from 5 min to 30 min.…”

Section: Determination Of Biogenic Amines In Real Samplesmentioning

confidence: 99%

High-throughput sensing microtiter plate for determination of biogenic amines in seafood using fluorescence or eye-vision

Azab

El-Korashy

Anwar

et al. 2011

Analyst

View full text Add to dashboard Cite

A new optical sensing microplate was developed for rapid screening for the presence of biogenic amines (BAs) in seafood samples with high sensitivity. The deposition of a sensing spot (containing a chameleon dye (Py-1) in a polymeric cocktail) on the bottom of the wells of a standard microplate renders the plate a new sensing tool for a rapid and parallel detection of up to 96 (real) samples. This sensing microplate enables (1) a semi-quantitative readout of analyte concentration by eye-vision, (2) a rapid fluorescence readout of 96 samples with standard instrumentation in less than two minutes (unlike chromatographic and electrophoretic methods), (3) a statistically robust data evaluation (with 8-12 replicates) and (4) a rapid parallel sample preparation with standard 8 or 12-channel micropipettes. On reaction with biogenic amines, the dye shows a significant visible color change from blue over green to red color. The appearance of red color favorably coincides with the concentration of BAs that can induce symptoms of poisoning. The linear ranges of fluorescence calibration data for six biogenic amines cover the clinical toxicological relevant range of BAs that is too low to be detected by the human nose. The LODs range from 0.16 to 0.56 mg mL À1, with correlation coefficients (r 2 ) between 0.985 and 0.999. Finally, the evolution of spoilage of four fish samples (monitored by determination of their BA status) and the increase of their total amine content were found to agree well with previous data on time-dependent evolution of BAs in fish.

show abstract

“…However, these analytical techniques require the use of a derivatization step involving trifluroacetylacetone [5], ophthaldehyde/thiol [16], dabsyl chloride [17] or 3-5-dinitrobenzoyl chloride [18] prior to or after separation in order to achieve a sensitive detection. Monoamine oxidase, EC 1.4.3.4 [9], diamine oxidase, EC 1.4.3.6 [8,19,20] and putrescine oxidase, EC 1.4.3.10 [3,8,20] have been shown to react with biogenic amines with a certain degree of substrate specificity, depending upon the enzyme source. For both diamine and putrescine oxidases, the enzymatic reaction of the diamines involves an oxidative deamination step with the liberation of ammonia and hydrogen peroxide:…”

Section: Introductionmentioning

confidence: 98%

“…Therefore, the reaction can be monitored by measuring H 2 O 2 with a Pt electrode [1,6,9]. However, this electrochemical measurement requires such a high potential (600 ± 700 mV) that it also detects other endogenous interferences.…”

Section: Introductionmentioning

confidence: 99%

“…Biogenic amine detection is also a valuable tool for assessing the freshness and quality of a wide variety of foodstuffs [6 ± 10]. Histamine, putrescine and cadaverine are produced via microbial decarboxylation of amino acids during food spoilage of packaged fruits [6], meat [7] and fish [8,9]. Biogenic amines are also formed during the bioprocess of alcoholic beverages, including beer [10] and wine [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An Electrochemical Flow Analysis System for Putrescine Using Immobilized Putrescine Oxidase and Horseradish Peroxidase

2004

View full text Add to dashboard Cite

An electrochemical flow analysis system was optimized together with immobilized putrescine oxidase and horseradish peroxidase for putrescine measurement. Four coupling agents, suberic acid bis(N-hydroxysuccinimide ester), gmaleimidobutyric N-hydroxysuccinimide ester, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide and glutaraldehyde, were used for immobilizing the two enzymes on porous aminopropyl glass beads to form a bienzymic detection column. Although the glutaraldehyde crosslinking procedure offered the highest response, the immobilized bienzyme system was responsive to putrescine, spermidine (123% of the putrescine response at 250 mM) and cadaverine (98% of the putrescine response). In contrast, the enzymes immobilized on the glass beads using suberic acid bis(Nhydroxysuccinimide ester) offered significantly better selectivity towards putrescine at the same concentration. For comparison, cadaverine and spermidine only provoked a response of 4.7% and 27.5% of the putrescine signal. The response to cadaverine and spermidine was further suppressed by lowering the detection pH from 8 to 7. At 250 mM, the response obtained for cadaverine and spermidine was only 1.5% and 3.9%, respectively, of the signal obtained for putrescine. A linear response to putrescine was obtained from 5 to 75 mM (0.629 mAs mM À1 , R 2 0.997) with a detection limit of 5 mM (S/N 3). The amperometric response retained 75% of its initial value after 600 repeated injections. The immobilized PUO/HRP (putrescine oxidase/horseradish peroxidase) was successfully demonstrated for measuring putrescine in fish extracts as an indicator of fish spoilage.

show abstract

Development of screen-printed enzyme electrodes for the estimation of fish quality

Cited by 52 publications

References 13 publications

Multiwall Carbon Nanotube (MWCNT) Based Electrochemical Biosensors for Mediatorless Detection of Putrescine

Multiwall Carbon Nanotube (MWCNT) Based Electrochemical Biosensors for Mediatorless Detection of Putrescine

High-throughput sensing microtiter plate for determination of biogenic amines in seafood using fluorescence or eye-vision

An Electrochemical Flow Analysis System for Putrescine Using Immobilized Putrescine Oxidase and Horseradish Peroxidase

Contact Info

Product

Resources

About