Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea

Pande, S.; Sharma, Mamta; Gaur, Pooran M.; Tripathi, Shailesh; Kaur, Livinder; Basandrai, Ashwani K.; Khan, Tanveer; Gowda, C L L; Siddique, Kadambot H. M.

doi:10.1007/s13313-010-0024-8

Cited by 47 publications

(66 citation statements)

References 15 publications

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“…Sharma et al (2006) also found a high correlation between greenhouse (seedling stage) and field screening (adult plant) for AB resistance. This supports the evaluation for AB resistance using 10-day-old-seedlings in a controlled environment at ICRISAT and adult plant field screening at hot-spot locations in Dhaulakuan and Ludhiana in India (Pande et al 2009). Resistance to AB has been one of the major objectives in breeding programs.…”

Section: Discussionsupporting

confidence: 74%

Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight

2010

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Ascochyta blight (AB, Ascochyta rabiei (Pass.) Lab.) is one of the most important foliar disease of chickpea (Cicer arietinum L.), globally. Chickpea is attacked by AB at any growth stage in cool and humid weather depending on the inoculum availability. However, the disease epidemics are most prominent during the flowering and podding growth stages. The main objective of this study was to determine the effect of growth stages of chickpea on the genetic resistance of AB and use this information in a resistance breeding program. Two susceptible and two moderately resistant chickpea cultivars were spray inoculated at seedling (GS1), post-seedling (GS2), vegetative (GS3), flowering (GS4) and podding (GS5) growth stages with A. rabiei conidial suspension under controlled environment conditions. Irrespective of crop cultivars the incubation period (IP) was shorter in GS1, GS4 and GS5 and was significantly extended in GS2 and GS3. Symptom development was delayed significantly in moderately resistant cultivars. The AB severity 10 days after inoculation ranged between 7 and 9 on susceptible cultivars and 3 and 5 on moderately resistant cultivars. Further the correlation coefficient of disease severity between GS1, GS4 and GS5 was highly significant (r=0.95) indicating that, evaluation for resistance to AB can be done at GS 1 (seedling stage), and or GS4 (flowering stage) to GS5 (podding stage) growth stages of chickpea. This supports the evaluation for AB resistance using 10-day-old-seedlings in controlled environment at ICRISAT and adult plant field screening at hot-spot locations in Dhaulakuan and Ludhiana in India.

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Section: Discussionsupporting

confidence: 74%

Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight

2010

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“…This facilitates the greatest discrimination among the lines in a trial. Screening techniques such as seedling screening and cut twig screening using excised trays are routinely being used to screen chickpea genotypes against AB in the controlled environment growth chamber facility at ICRISAT, Patancheru India [48]. Chen and Muehlbauer [49] developed a mini-dome technique for AB resistance screening and this technique is in use at Pullman, WA, USA.…”

Section: Genetic Resources Of Ab Resistancementioning

confidence: 99%

“…A resistant check/ susceptible was included in order to compare resistance of test entries with that of known resistant material [51]. A positive correlation between a field and controlled environment screening technique for AB was reported by Pande et al [48].…”

Section: Genetic Resources Of Ab Resistancementioning

confidence: 99%

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An Update on Genetic Resistance of Chickpea to Ascochyta Blight

Sharma

Ghosh

2016

Agronomy

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Ascochyta blight (AB) caused by Ascochyta rabiei (Pass.) Labr. is an important and widespread disease of chickpea (Cicer arietinum L.) worldwide. The disease is particularly severe under cool and humid weather conditions. Breeding for host resistance is an efficient means to combat this disease. In this paper, attempts have been made to summarize the progress made in identifying resistance sources, genetics and breeding for resistance, and genetic variation among the pathogen population. The search for resistance to AB in chickpea germplasm, breeding lines and land races using various screening methods has been updated. Importance of the genotypeˆenvironment (GE) interaction in elucidating the aggressiveness among isolates from different locations and the identification of pathotypes and stable sources of resistance have also been discussed. Current and modern breeding programs for AB resistance based on crossing resistant/multiple resistant and high-yielding cultivars, stability of the breeding lines through multi-location testing and molecular marker-assisted selection method have been discussed. Gene pyramiding and the use of resistant genes present in wild relatives can be useful methods in the future. Identification of additional sources of resistance genes, good characterization of the host-pathogen system, and identification of molecular markers linked to resistance genes are suggested as the key areas for future study.

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“…To overcome some of these genetic limitations for disease resistance in modern C. arietinum cultivars, resistance has been sought among wild relatives for a number of diseases (Singh et al 2008), including chickpea cyst nematode Heterodera ciceri (Di Vito et al 1996), and two important fungal diseases affecting chickpeas in Australia, namely Ascochyta blight (Collard et al 2001;Pande et al 2011) and Phytophthora root rot ). More specifically, Di Vito et al (1995) reported resistance to P. thornei in accessions of C. bijugum and C. judaicum (both in the secondary gene pool) and C. cuneatum Hochst.…”

Section: Introductionmentioning

confidence: 99%

Hybridisation of Australian chickpea cultivars with wild Cicer spp. increases resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus)

Thompson¹,

Reen²,

Clewett³

et al. 2011

Australasian Plant Pathol.

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Australian and international chickpea (Cicer arietinum) cultivars and germplasm accessions, and wild annual Cicer spp. in the primary and secondary gene pools, were assessed in glasshouse experiments for levels of resistance to the root-lesion nematodes Pratylenchus thornei and P. neglectus. Lines were grown in replicated experiments in pasteurised soil inoculated with a pure culture of either P. thornei or P. neglectus and the population density of the nematodes in the soil plus roots after 16 weeks growth was used as a measure of resistance. Combined statistical analyses of experiments (nine for P. thornei and four for P. neglectus) were conducted and genotypes were assessed using best linear unbiased predictions. Australian and international chickpea cultivars possessed a similar range of susceptibilities through to partial resistance. Wild relatives from both the primary (C. reticulatum and C. echinospermum) and secondary (C. bijugum) gene pools of chickpea were generally more resistant than commercial chickpea cultivars to either P. thornei or P. neglectus or both. Wild relatives of chickpea have probably evolved to have resistance to endemic rootlesion nematodes whereas modern chickpea cultivars constitute a narrower gene pool with respect to nematode resistance. Resistant accessions of C. reticulatum and C. echinospermum were crossed and topcrossed with desi chickpea cultivars and resistant F 4 lines were obtained. Development of commercial cultivars with the high levels of resistance to P. thornei and P. neglectus in these hybrids will be most valuable for areas of the Australian grain region and other parts of the world where alternating chickpea and wheat crops are the preferred rotation.

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Development of screening techniques and identification of new sources of resistance to Ascochyta blight disease of chickpea

Cited by 47 publications

References 15 publications

Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight

Effect of growth stages of chickpea on the genetic resistance of Ascochyta blight

An Update on Genetic Resistance of Chickpea to Ascochyta Blight

Hybridisation of Australian chickpea cultivars with wild Cicer spp. increases resistance to root-lesion nematodes (Pratylenchus thornei and P. neglectus)

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