Development of sensitive multiwavelength fluorescence detector system for high-performance liquid chromatography

Abstract

Cu(I1) and the other with 5% Cu(I1). The response of the calcein blue reagent to these solutions was obtained, and the Stern-Volmer quenching parameters thus measured are collected in Table IV. It was concluded that the addition of 2% Cu(I1) yielded only a 0.4% error in computed Gd(II1) concentrations, while the addition of 5% Cu(I1) yielded only a 1.3% error.Apparent association constants for the transition-metal complexes with calcein blue and methyl calcein blue were computed from the quenching data by using the same analysis c o 24 060 a 380 as previously described. These constants are found in Table V, and comparison with analogous iminodiacetate and amino acid complexes (9) indicates that the dye complexes are less stable than those of the parent chelating group. This observation implies that the steric bulk of the umbelliferone group interferes with the complexation process, as had been inferred for the lanthanide-dye complexes. LITERATURE CITEDHowell, J. A.; Hargis, L. G. Anal. Chem.