Development of Sequence Characterized Amplified Region Markers for Cultivar Identification in Persimmon

Abstract

Abstract. Precise, fast, and cost-effective identification of crop cultivars is essential for plant breeder's rights. Traditional methods for identification of persimmon cultivars are based on the evaluation of sets of morphological characteristics. However, it is difficult to distinguish closely related cultivars using only morphological traits. This study was conducted to develop DNA markers for identification of the 32 persimmon cultivars in Korea and Japan. A total of 309 randomly amplified polymorphic DNA (RAPD) markers were identified using 40 different random primers. Various number of polymorphic bands ranged from 4 (OPP-08) to 14 (UBC159) were detected with an average of 7.7. Resulting 57 RAPD fragments were selected, and their sequences were determined for developing sequence characterized amplified region (SCAR) markers. As a result, 15 of 57 RAPD fragments were successfully converted to SCAR markers. Single polymorphic bands of the same size as or smaller than the RAPD fragments were amplified depending on SCAR markers. Among these markers, a combination of eight SCAR markers (PS225_200, PSN05_420, PSF13_523, PSN11_540, PS372_567, PS485_569, PSP08_635, and PS631_735) provided sufficient polymorphisms to identify 32 persimmon cultivars. These newly developed markers will be a fast and reliable tool to identify persimmon cultivars. Additional key words:

Origen, dispersión y diversidad del chirimoyo (Annona cherimola Mill.) en el continente americano

“…Additional PCR-based markers used to some extent are inter-simple sequence repeats (ISSRs), applied, for example, in fig (Amel et al 2004(Amel et al , 2005Ikegami et al 2009) or clementine (Breto et al 2001); cleaved amplified polymorphisms (CAPs) used, for example, in Citrus spp. (Amar et al 2011); sequenced characterized amplified regions (SCARs) used, for example, in persimmon (Cho et al 2013); or sequence-tagged sites and expressed sequence tags (STS and ESTs, respectively) used, for example, in Musa acuminata (Passos et al 2012) or Actinidia spp. (Crowhurst et al 2008).…”