Development of single locus DNA microsatellite markers in Oryctes rhinoceros (Linnaeus) using 5′ anchored RAMs-PCR method

Manjeri, G.; Muhamad, Rita; Faridah, Q. Z.; Tan, Soon Guan

doi:10.1007/s12041-012-0189-8

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Although specific anchor primers of terminal microsatellite markers had shown successful amplification of targeted loci and applicable for genotyping on gels [13–15], none of these studies genotype the samples by capillary electrophoresis. From the result, we have shown that even though microsatellite markers isolated using the protocols described could be detected in the fluorescent capillary electrophoresis, the quality of peak pattern varied across samples.…”

Section: Resultsmentioning

confidence: 99%

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A revisit to a low-cost method for the isolation of microsatellite markers: the case of the endangered Malayan tapir (Tapirus indicus)

Lim

Ismail

Arumugam

et al. 2018

Preprint

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There are many approaches to develop microsatellite markers. We revisited an easy and rapid Polymerase Chain Reaction (PCR)-cloning-sequencing method to design microsatellite markers for Tapirus indicus. Using six random amplified microsatellite (RAM) markers, this study had rapidly generated 45 unique genomic sequences containing microsatellites. After screening 15 terminal and seven intermediate microsatellite loci, we shortlisted five and seven which were amplified either by single- or multiplex PCR using the economical three-primer PCR method. Genotyping attempts were made with ten Tapirus indicus individuals using three of the terminal microsatellite loci and all seven intermediate loci. However, none of the terminal microsatellite loci were considered useful for population genotyping studies, while the seven intermediate loci showed good amplification but were monomorphic in the ten samples. Despite successful detection of amplified loci, we would like to highlight that, researchers who are interested in this alternative method for isolation of microsatellite loci to be cautious and be aware of the limitations and downfalls reported herein that could render these loci unsuitable for population genotyping.

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Section: Resultsmentioning

confidence: 99%

“…Next, specific primers can be designed for both the terminal and intermediate microsatellites. This technique has been used in the rapid generation of microsatellite markers in earlier studies [13–15].…”

Section: Introductionmentioning

confidence: 99%

A revisit to a low-cost method for the isolation of microsatellite markers: the case of the endangered Malayan tapir (Tapirus indicus)

Lim

Ismail

Arumugam

et al. 2018

Preprint

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Next-generation sequencing, isolation and characterization of 14 microsatellite loci of Canthon cyanellus (Coleoptera: Scarabaeidae)

et al. 2021

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BackgroundWe used Illumina paired-end-sequencing to isolate and characterize microsatellites of Canthon cyanellus, a Neotropical roller dung beetle encompassing several lineages within its distribution range. Methods and ResultsWe examined C. cyanellus specimens collected at eight different localities in Mexico (two or three specimens per locality). We initially made ampli cation tests with 16 loci, but two of these showed ampli cation problems; thus, we evaluated 14 loci. The 14 microsatellites were polymorphic, with 2-16 alleles each. The expected and observed heterozygosity ranged from 0.11 to 0.76 and from 0.20 to 0.78, respectively. ConclusionsThese microsatellites will help to assess structure at the population and lineage levels, identify zones of potential hybridization between lineages, and draw a more precise geographic delimitation of Canthon cyanellus lineages.

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Oryctes rhinoceros Beetles, an Oil Palm Pest in Malaysia

Manjeri

Muhamad

Tan

2014

ARRB

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Oryctes rhinoceros, commonly known as the rhinoceros beetle is an important agricultural pest that is known to inflict serious damage on young oil palm trees. Many researches have been conducted on its development, life cycle, habitat, management and genomic variation ever since the need to understand this pest arose. Oryctes rhinoceros is among the longest present agricultural pest in Malaysia and it has witnessed the formulation and implementation of various phases of control and management strategies. To date, research and development activities are still ongoing in Malaysia for the successful management of this pest. In this review, we look into details on the characteristics of this pest, the modes of its introduction into Malaysia, as well as the events that helped to establish and contribute to the proliferation of this pest as a major oil palm threat in Malaysia. The progressive development of various research and development activities concerning the management and control of this pest are also highlighted.

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Development of single locus DNA microsatellite markers in Oryctes rhinoceros (Linnaeus) using 5′ anchored RAMs-PCR method

Cited by 3 publications

References 11 publications

A revisit to a low-cost method for the isolation of microsatellite markers: the case of the endangered Malayan tapir (Tapirus indicus)

A revisit to a low-cost method for the isolation of microsatellite markers: the case of the endangered Malayan tapir (Tapirus indicus)

Next-generation sequencing, isolation and characterization of 14 microsatellite loci of Canthon cyanellus (Coleoptera: Scarabaeidae)

Oryctes rhinoceros Beetles, an Oil Palm Pest in Malaysia

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