Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection

Jo, Minjoung; Ahn, Ji‐Young; Lee, Joohyung; Lee, Seram; Hong, Sun Woo; Yoo, Jae-Wook; Kang, Ji-Hoon; Dua, Pooja; Lee, Dong-Ki; Hong, Seunghun; Kim, Soyoun

doi:10.1089/oli.2010.0267

Cited by 171 publications

(130 citation statements)

References 25 publications

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“…HAADF‐STEM (Figure 1b), elemental mapping (Figure 1c), and line scan analysis (Figure S4, Supporting Information) clearly demonstrated the well‐defined dumbbell‐shaped structures of Fe 3 O 4 –Ag Janus nanoparticles. The Ag surface was further functionalized with Hg 2+ ‐binding aptamers,15 and Fe 3 O 4 surface with BPA‐binding aptamers (Figure 1d) 16. The successful functionalization of Janus nanoparticle by aptamers was verified by zeta potential measurement (Figure S9, Supporting Information).…”

Section: Resultsmentioning

confidence: 95%

A Targeted “Capture” and “Removal” Scavenger toward Multiple Pollutants for Water Remediation based on Molecular Recognition

Wang

Shen

et al. 2015

Advanced Science

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For the water remediation techniques based on adsorption, the long‐standing contradictories between selectivity and multiple adsorbability, as well as between affinity and recyclability, have put it on weak defense amid more and more severe environment crisis. Here, a pollutant‐targeting hydrogel scavenger is reported for water remediation with both high selectivity and multiple adsorbability for several pollutants, and with strong affinity and good recyclability through rationally integrating the advantages of multiple functional materials. In the scavenger, aptamers fold into binding pockets to accommodate the molecular structure of pollutants to afford perfect selectivity, and Janus nanoparticles with antibacterial function as well as anisotropic surfaces to immobilize multiple aptamers allow for simultaneously handling different kinds of pollutants. The scavenger exhibits high efficiencies in removing pollutants from water and it can be easily recycled for many times without significant loss of loading capacities. Moreover, the residual concentrations of each contaminant are well below the drinking water standards. Thermodynamic behavior of the adsorption process is investigated and the rate‐controlling process is determined. Furthermore, a point of use device is constructed and it displays high efficiency in removing pollutants from environmental water. The scavenger exhibits great promise to be applied in the next generation of water purification systems.

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Section: Resultsmentioning

confidence: 95%

A Targeted “Capture” and “Removal” Scavenger toward Multiple Pollutants for Water Remediation based on Molecular Recognition

Wang

Shen

et al. 2015

Advanced Science

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“…An aptamer sequence toward BPA with a dissociation constant of 8.3 × 10 −9 M, which is even lower than that of an antibody, was used in the experiment. 25 The aptamer or linker DNA fragments were immobilized on magnetic beads according to the procedures of the xMag PDITC Oligo Immobilization Starter Kit. Uniformly distributed magnetic beads with an average particle size of ca.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing

Ding

et al. 2015

Anal. Chem.

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In this work, a simple, general, and sensitive potentiometric platform is presented, which allows potentiometric sensing to be applied to any class of molecule irrespective of the analyte charge. DNA nanostructures are self-assembled on magnetic beads via the incorporation of an aptamer into a hybridization chain reaction. The aptamer− target binding event leads to the disassembly of the DNA nanostructures, which results in a dramatic change in the surface charge of the magnetic beads. Such a surface charge change can be sensitively detected by a polycation-sensitive membrane electrode using protamine as an indicator. With an endocrine disruptor bisphenol A as a model, the proposed potentiometric method shows a wide linear range from 0.1 to 100 nM with a low detection limit of 80 pM (3σ). The proposed sensing strategy will lay a foundation for the development of potentiometric sensors for highly sensitive and selective detection of various targets.

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“…They are particularly attractive for sensor applications since they are relatively easily to produce with selectivity for a wide variety of analytes. These range from ions [122,123] or small molecules [124] to a wide range of proteins [125] or even entire cells [126,127]. As a result, aptamers Kleo et al [108] fabricated a DNA-QCM biosensor for indirect determination of vaccinia virus DNA using polymerase chain reaction (PCR) amplification.…”

Section: Aptamersmentioning

confidence: 99%

“…They are particularly attractive for sensor applications since they are relatively easily to produce with selectivity for a wide variety of analytes. These range from ions [122,123] or small molecules [124] to a wide range of proteins [125] or even entire cells [126,127]. As a result, aptamers can be used for detecting viruses directly as well as molecules produced by the affected host in response to the viral infection (as for instance antibodies or certain molecules produced on the host cell surface [128]).…”

Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Development of Single-Stranded DNA Aptamers for Specific Bisphenol A Detection

Cited by 171 publications

References 25 publications

A Targeted “Capture” and “Removal” Scavenger toward Multiple Pollutants for Water Remediation based on Molecular Recognition

A Targeted “Capture” and “Removal” Scavenger toward Multiple Pollutants for Water Remediation based on Molecular Recognition

DNA Nanostructure-Based Magnetic Beads for Potentiometric Aptasensing

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

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