Development of spectrofluorimetric method for determination of certain antiepileptic drugs through condensation with ninhydrin and phenyl acetaldehyde

Derayea, Sayed M.; Attia, Tamer Z.; Elnady, Mohamed

doi:10.1016/j.saa.2018.06.027

Cited by 19 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The samples were withdrawn at intervals of (5,15,30,60,90,120,150,180,210,240,300,360,420,480, and 1440 min) [20]. To each Pregabalin sample, ninhydrin solution (1%) prepared in DMF was added [22,23]. After that, this mixture was heated in a water bath at 70 • C for 15-20 min.…”

Section: In Vitro Drug Release Studies Of Topical Gelsmentioning

confidence: 99%

Co-Combination of Pregabalin and Withania coagulans-Extract-Loaded Topical Gel Alleviates Allodynia and Hyperalgesia in the Chronic Sciatic Nerve Constriction Injury for Neuropathic Pain in Animal Model

Asghar

Aamir

Sheikh³

et al. 2022

Molecules

View full text Add to dashboard Cite

The current study reports the fabrication of co-combination gel using Pregabalin and Withania coagulans fruit extract to validate its effectiveness for neuropathic pain in chronic constriction injury (CCI) rat models. Three topical gels were prepared using Carbopol 934 through a pseudo-ternary phase diagram incorporating the Pregabalin (2.5%), Withania coagulans extract (2%), and co-combination of both Pregabalin (2.5%) and Withania coagulans extract (2%). Gels were characterized. FTIR showed a successful polymeric network of the gel without any interaction. The drug distribution at the molecular level was confirmed by XRD. The AFM images topographically indicated the rough surface of gels with a size range from 0.25 to 330 nm. DSC showed the disappearance of sharp peaks of the drug and extract, showing successful incorporation into the polymeric network of gels. The in vitro drug release of co-combination gel was 73% over 48 h. The mechanism of drug release by combination gel was Higuchi+ fickian with values of n (0.282) and R2 (0.947). An in vivo study for pain assessment via four methods: (i) heat hyperalgesia, (ii) cold allodynia, (iii) mechano-hyperalgesia, and (iv) dynamic mechano-allodynia, confirmed that topical treatment with co-combination gel reduced the pain significantly as indicated by the p value: R1 (p < 0.001), R2 (p < 0.001), R3 (p < 0.015), and R4 (p < 0.0344). The significance order was R2 (****) > R1 (***) > R3 (**) > R4 (*) > R5 (ns).

show abstract

Section: In Vitro Drug Release Studies Of Topical Gelsmentioning

confidence: 99%

Co-Combination of Pregabalin and Withania coagulans-Extract-Loaded Topical Gel Alleviates Allodynia and Hyperalgesia in the Chronic Sciatic Nerve Constriction Injury for Neuropathic Pain in Animal Model

Asghar

Aamir

Sheikh³

et al. 2022

Molecules

View full text Add to dashboard Cite

show abstract

“…[ 41 ] In this method, the fluorogenic agents (ninhydrin and phenylacetaldehyde) were reacted with the amino group of amikacin in the presence of Teorell and Stenhagen buffer at 66°C to produce a fluorescent product. [ 22,42 ] There have been a few reported analytical methods in which the drugs that lack chromophore were derivatized with fluorogenic agents (phenylacetaldehyde and ninhydrin) for quantification purposes, using a conventional fluorescence spectrometer. Although there has been a previously published work by Omar and colleagues (2015) following the same derivatization procedure for amikacin, upon extrapolating the method in our laboratory, the reported sensitivity and reproducibility were not achieved.…”

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

et al. 2022

View full text Add to dashboard Cite

An aminoglycoside antibiotic, amikacin, is used to treat severe and recurring bacterial infections. Due to the absence of a chromophore, however, amikacin must be extensively derivatized before being quantified, both in analytical and bioanalytical samples. In this study, for the first time, we developed a simple and sensitive method for measuring amikacin sulfate using spectrofluorimetry with a 96-well plate reader, based on the design of the experiment's approach. To develop a robust and reproducible spectrofluorimetric method, the influence of essential attributes, namely pH of the buffer, heating temperature, and concentration of reagents, were evaluated using univariate analysis followed by multivariate analysis (central composite design).International Conference of Harmonization guidelines were used to validate the optimized method. The developed technique is linear from 1.9 to 10 μg/ml with a regression coefficient of 0.9991. The detection and quantification limits were 0.649 μg/ml and 1.9 μg/ml, respectively. For the developed method, both intraday and interday precision (%RSD) were less than 5%. Using the method, amikacin concentrations were quantified in prepared amikacin liposomes and commercial formulations of Amicin ® . The developed method greatly reduces sample volume and is a rapid, high throughput microplate-based fluorescence approach for the convenient and costeffective measurement of amikacin in pharmaceutical formulations. In comparison with previously published approaches, the suggested method allowed for quick analysis of a high number of samples in a short amount of time (96 samples in 125 sec), resulting in an average duration of analysis of 1.3 sec per sample.

show abstract

“…These methods included liquid chromatography, [4][5][6][7] gas chromatography-mass spectrophotometry (GC-MS), [8] capillary electrophoresis and nuclear magnetic resonance, [9] liquid chromatography-mass spectrometry (LC-MS/MS), [10] spectrophotometry [11,12] and spectrofluorimetry. [13,14] However, these methods suffer from either low sensitivity, difficulty or high analysis cost. That is why a new simple, cheap and sensitive method is needed for analysis of PG in biological matrices and its commercially dosage form.…”

Section: Introductionmentioning

confidence: 99%

Carbon quantum dots as a sensitive fluorescent probe for quantitation of pregabalin; application to real samples and content uniformity test

et al. 2021

View full text Add to dashboard Cite

A novel optical nano-sensor for the detection of pregabalin (PG) in its pharmaceutical (Lyrica ® ) capsules and biological samples was reported. For the fabrication of highly fluorescent carbon quantum dots (CQDts), a simple green hydrothermal approach was described, and ascorbic acid (AA) was used as a carbon source. The obtained CQDts were confirmed by spectroscopic characterization such as transmission electron microscopy (TEM) and Fourier-transform infrared (FTIR) spectra.The synthesized CQDts were capped by alcohol to form yellow emitters, showing strong fluorescent emission at 524 nm, and excitation at 356 nm. The method is based on fluorescence quenching of CQDts in the presence of PG. The proposed analytical method was validated according to ICH guidelines. PG was successively assayed in the concentration range of 4.0 to 100 μg/ml). The detection and quantitation limits were 1.12 and 3.39 μg/ml, respectively. The proposed method could be used in both quality control and pharmacokinetic research for the studied drug.

show abstract

Development of spectrofluorimetric method for determination of certain antiepileptic drugs through condensation with ninhydrin and phenyl acetaldehyde

Cited by 19 publications

References 28 publications

Co-Combination of Pregabalin and Withania coagulans-Extract-Loaded Topical Gel Alleviates Allodynia and Hyperalgesia in the Chronic Sciatic Nerve Constriction Injury for Neuropathic Pain in Animal Model

Co-Combination of Pregabalin and Withania coagulans-Extract-Loaded Topical Gel Alleviates Allodynia and Hyperalgesia in the Chronic Sciatic Nerve Constriction Injury for Neuropathic Pain in Animal Model

A novel high throughput 96‐well‐based fluorimetric method to measure amikacin in pharmaceutical formulations: development using response surface methodology

Carbon quantum dots as a sensitive fluorescent probe for quantitation of pregabalin; application to real samples and content uniformity test

Contact Info

Product

Resources

About