Development of SSR markers for <i>Encholirium horridum</i> (Bromeliaceae) and transferability to other Pitcairnioideae

Hmeljevski, Karina Vanessa; Ciampi, Maísa B.; Baldauf, Cristina; Reis, Maurício Sedrez dos; Forzza, Rafaela Campostrini

doi:10.3732/apps.1200445

Cited by 8 publications

(10 citation statements)

References 7 publications

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“…Eight nuclear microsatellite (nSSR) loci developed for E. horridum (EhA07, EhB09, EhC03, EhE01, EhE02, EhE11, EhG03, EhG07; Hmeljevski, Ciampi, Baldauf, Reis, & Forzza, ) were genotyped. For cpDNA, the transferability of plastid microsatellites (cpSSR) isolated from Vriesea gigantea Mart.…”

Section: Methodsmentioning

confidence: 99%

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Do plant populations on distinct inselbergs talk to each other? A case study of genetic connectivity of a bromeliad species in an Ocbil landscape

Hmeljevski

Nazareno

Bueno

et al. 2017

Ecology and Evolution

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Here, we explore the historical and contemporaneous patterns of connectivity among Encholirium horridum populations located on granitic inselbergs in an Ocbil landscape within the Brazilian Atlantic Forest, using both nuclear and chloroplast microsatellite markers. Beyond to assess the E. horridum population genetic structure, we built species distribution models across four periods (current conditions, mid‐Holocene, Last Glacial Maximum [LGM], and Last Interglacial) and inferred putative dispersal corridors using a least‐cost path analysis to elucidate biogeographic patterns. Overall, high and significant genetic divergence was estimated among populations for both nuclear and plastid DNA (ΦST (n) = 0.463 and ΦST (plastid) = 0.961, respectively, p < .001). For nuclear genome, almost total absence of genetic admixture among populations and very low migration rates were evident, corroborating with the very low estimates of immigration and emigration rates observed among E. horridum populations. Based on the cpDNA results, putative dispersal routes in Sugar Loaf Land across cycles of climatic fluctuations in the Quaternary period revealed that the populations’ connectivity changed little during those events. Genetic analyses highlighted the low genetic connectivity and long‐term persistence of populations, and the founder effect and genetic drift seemed to have been very important processes that shaped the current diversity and genetic structure observed in both genomes. The genetic singularity of each population clearly shows the need for in situ conservation of all of them.

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Section: Methodsmentioning

confidence: 99%

“…Fluorescent‐labeled primers were used for genotyping reactions, following protocols described by Hmeljevski et al. (). Both nSSR and cpSSR alleles were resolved in an ABI 3500xL automatic sequencer (Applied Biosystems, Foster City, CA, USA) using GSLIZ600 as the size standard (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Do plant populations on distinct inselbergs talk to each other? A case study of genetic connectivity of a bromeliad species in an Ocbil landscape

Hmeljevski

Nazareno

Bueno

et al. 2017

Ecology and Evolution

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“…In most cases, this can be attributed to the presence of inbreeding or to the Wahlund effect (Hartl and Clark, 1997). In populations with the highest number of loci with deviations (A. ramosa and B. euphemia), significant values were also found for fixation indexes, giving positive values for intrapopulation inbreeding.…”

Section: Discussionmentioning

confidence: 99%

“…Plants in the Bromeliaceae family provide excellent models for the study of genetic diversity (Zanella et al, 2012;Hmeljevski et al, 2013), population genetics (Lavor et al, 2014), as well as the divergence between populations and species (Sarthou et al, 2001;Barbará et al, 2007;Palma-Silva et al, 2009). Bromeliads are an important and characteristic element of Neotropical forests, especially in the Brazilian Atlantic Forest (BAF), where they are among those families with the greatest richness and diversity (Martinelli et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, research on population genetic diversity is essential to provide information on the risk of species extinction and to design effective conservation strategies (Frankham et al, 2002). In this context, molecular markers are widely used for studying genetic variability (Cavallari et al, 2006;Barbará et al, 2007Barbará et al, , 2009Lavor et al, 2014;Goetze et al, 2015), and several markers have been developed for Bromeliaceae: Pitcairnia (Sarthou et al, 2003;Paggi et al, 2008), Alcantarea , Dyckia (Zanella et al, 2012), Encholirium (Hmeljevski et al, 2013), Orthophytum (Aoki-Gonçalves et al, 2014), and recently, Vriesea (Neri et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of Bromeliaceae species from the Atlantic Forest

Sheu

Cunha-Machado²,

Gontijo

et al. 2017

Genet. Mol. Res.

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ABSTRACT. The Bromeliaceae family includes a range of species used for many purposes, including ornamental use and use as food, medicine, feed, and fiber. The state of Espírito Santo, Brazil is a center of diversity for this family in the Atlantic Forest. We evaluated the genetic diversity of five populations of the Bromeliaceae family, including specimens of the genera Aechmea, Billbergia (subfamily Bromelioideae), and Pitcairnia (subfamily Pitcairnioidea), all found in the Atlantic Forest and distributed in the state of Espírito Santo. The number of alleles per locus in populations ranged from two to six and the fixation index (F), estimated for some simple sequence repeats in bromeliad populations, was less than zero in all populations. All markers in the Pitcairnia flammea population were in Hardy-Weinberg equilibrium (P < 0.05). Moreover, significant deviations from Hardy-Weinberg equilibrium were observed at some loci in populations of the five bromeliad species. In most cases, this can be attributed to the presence of inbreeding or the Wahlund effect. The genetic diversity indices of five species showed greater allelic richness in P. flammea (3.55). Therefore, we provide useful information for the characterization of genetic diversity in natural populations of Aechmea ramosa, Aechmea nudicaulis, Billbergia horrid, Billbergia euphemia, and P. flammea in Atlantic Forest remnants in the south of Espírito Santo state.

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Cross-amplification of nuclear microsatellite markers in two species of Cryptanthus Otto & A. Dietr. (Bromeliaceae)

et al. 2017

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Thirty-eight nuclear microsatellite loci originally developed for Aechmea caudata Lindm., Orthophytum ophiuroides Louzada & Wand., Pitcairnia albiflos Herb., Vriesea gigantea (Gaud.) and V. simplex (Vell.) Beer were tested in Cryptanthus burle-marxii Leme and C. zonatus (Vis.) Vis. Of the 38 loci tested, 13 were polymorphic. Ten polymorphic microsatellite loci were selected to be amplified and genotyped in one population each of C. burle-marxii and C. zonatus. The observed and expected heterozygosity per locus in the C. burle-marxii population ranged from 0.050 to 0.850 and 0.050 to 0.770, respectively. In C. zonatus, the observed and expected heterozygosity per locus ranged from 0.167 to 0.846 and 0.290 to 0.692, respectively. The O. ophiuroides locus Op52 for the C. zonatus population and P. albiflos locus PaC05 for the two species showed significant departure from HWE. These ten polymorphic loci tested will be used to assess the genetic diversity and structure of the two species of Cryptanthus.

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Development of SSR markers for Encholirium horridum (Bromeliaceae) and transferability to other Pitcairnioideae

Cited by 8 publications

References 7 publications

Do plant populations on distinct inselbergs talk to each other? A case study of genetic connectivity of a bromeliad species in an Ocbil landscape

Do plant populations on distinct inselbergs talk to each other? A case study of genetic connectivity of a bromeliad species in an Ocbil landscape

Genetic diversity of Bromeliaceae species from the Atlantic Forest

Cross-amplification of nuclear microsatellite markers in two species of Cryptanthus Otto & A. Dietr. (Bromeliaceae)

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