Development of the digestive tract, trypsin activity and gene expression in eggs and larvae of the bullseye puffer fish Sphoeroides annulatus

García‐Gasca, Alejandra; Galavíz, Mario A.; Gutiérrez, Jesús N.; García‐Ortega, Armando

doi:10.1016/j.aquaculture.2005.05.029

Cited by 74 publications

(51 citation statements)

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“…The development of the digestive tract and associated organs has been well documented for a number of marine and freshwater species (Verreth et al 1992;Segner et al 1994;Peña et al 2003;Gisbert et al 2004;Mai et al 2005;Chen et al 2006;García-Gasca et al 2006;Rønnestad and Morais 2008;ZamboninoInfante et al 2008;Galaviz et al 2011). However, only few studies combine molecular and biochemical procedures to describe the relationship between the transcription of a digestive enzyme and its corresponding enzymatic activity, as reported for spotted sand bass Paralabrax maculatofasciatus (Á lvarez- González et al 2008González et al , 2010, winter flounder Pleuronectus americanus (Douglas et al 1999;Murray et al 2004Murray et al , 2006, bullseye puffer Sphoeroides annulatus (García-Gasca et al 2006), Atlantic salmon Salmo salar (Rungruangsak-Torrissen et al 2006), turbot Scophthalmus maximus (Chi et al 2013) and Asian seabass Lates calcarifer (Srichanun et al 2013). Studies integrating the development of the digestive system with expression or activation of digestive enzymes during development of larvae are scarce (Péres et al 1998;Cahu et al 2004;García-Gasca et al 2006;Gisbert et al 2009;Galaviz et al 2011Galaviz et al , 2012.…”

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confidence: 99%

Digestive system development and study of acid and alkaline protease digestive capacities using biochemical and molecular approaches in totoaba (Totoaba macdonaldi) larvae

Galavíz

López

Gasca

et al. 2015

Fish Physiol Biochem

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The present study aimed to describe and understand the development of the digestive system in totoaba (Totoaba macdonaldi) larvae from hatching to 40 days post-hatch (dph) from morphological and functional perspectives. At hatch, the digestive system of totoaba was undifferentiated. The anus and the mouth opened at 4 and 5 dph, respectively. During exogenous feeding, development of the esophagus, pancreas, liver and intestine was observed with a complete differentiation of all digestive organs. Expression and activity of trypsin and chymotrypsin were observed as early as at 1 dph, and increments in their expression and activity coincided with changes in food items (live and compound diets) and morpho-physiological development of the accessory digestive glands. In contrast, pepsin was detected later during development, which includes the appearance of the gastric glands between 24 and 28 dph. One peak in gene expression was detected at 16 dph, few days before the initial development of the stomach at 20 dph. A second peak of pepsin expression was detected at day 35, followed by a peak of activity at day 40, coinciding with the change from live to artificial food. Totoaba larvae showed a fully morphologically developed digestive system between 24 and 28 dph, as demonstrated by histological observations. However, gene expression and activity of alkaline and acid proteases were detected earlier, indicating the functionality of the exocrine pancreas and stomach before the complete morphological development of the digestive organs. These results showed that integrative studies are needed to fully understand the development of the digestive system from a morphological and functional point of views, since the histological organization of digestive structures does not reflect their real functionality. These results indicate that the digestive system of totoaba develops rapidly during the first days post-hatch, especially for alkaline proteases, and the stomach becomes functional between 20 and 24 dph allowing the weaning process to begin at this age.

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confidence: 99%

Digestive system development and study of acid and alkaline protease digestive capacities using biochemical and molecular approaches in totoaba (Totoaba macdonaldi) larvae

Galavíz

López

Gasca

et al. 2015

Fish Physiol Biochem

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“…Este porcentaje de supervivencia es superior al reportado hasta entonces (Álvarez Lajonchere et al 2008, Abdo-de la Parra et al 2010. García-Gasca et al (2006) examinaron el desarrollo del tracto digestivo para evaluar la capacidad digestiva de larvas de botete diana desde la eclosión hasta 32 DDE. El desarrollo del tracto digestivo se analizó por histología y la actividad de la tripsina, mediante histoquímica.…”

Section: Efecto Del Desgomado De Huevos En La Larviculturaunclassified

Biotecnología para la producción masiva de juveniles del botete diana Sphoeroides annulatus: inducción hormonal y cultivo larvario

Parra

Rodríguez-Ibarra

García-Aguilar³

et al. 2013

Rev. biol. mar. oceanogr.

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Abstract.-The bullseye puffer fish Sphoeroides annulatus is an important marine species due to its culture potential and high market value in Mexico. Research on aspects of reproduction and larvae culture of this puffer fish commenced in México in 1997 at Centro de Investigación en Alimentación y Desarrollo (CIAD-Mazatlan Unit), Mazatlán, Sinaloa. This work describes the following biotechnological developments for obtaining mass production of the bullseye puffer fish juveniles: hormonal induction methods, spawning, egg fertilization and incubation, culture of live feed and larviculture.Key words: Bullseye puffer fish, Sphoeroides annulatus, biotechnology, larvicultureResumen.-El botete diana Sphoeroides annulatus es un pez marino con alto valor en el mercado mexicano con potencial para su cultivo. Las investigaciones sobre los aspectos reproductivos y cultivo de larvas de esta especie, iniciaron en México a partir de 1997 en el Centro de Investigación y Alimentación (CIAD), Unidad Mazatlán. El presente trabajo describe el desarrollo de la biotecnología para la producción masiva de juveniles; la cual incluye los protocolos para la inducción hormonal, desove, fertilización e incubación de huevos, cultivo de alimento vivo y cultivo de larvas.Palabras clave: Botete diana, Sphoeroides annulatus, biotecnología, larvicultura INTRODUCCIÓNMéxico es un país que tiene un enorme potencial para el cultivo de peces marinos; cuenta con especies con un alto valor comercial y con condiciones medioambientales apropiadas para desarrollar una maricultura sustentable. Sin embargo, hasta la fecha sólo el cultivo de camarón y de algunos moluscos se realizan a escala comercial. En cuanto al cultivo de peces marinos en el litoral del Pacífico se han desarrollado recientemente algunas biotecnologías para la producción de juveniles para su engorda en jaulas como es el caso del botete diana Sphoeroides annulatus (Jenyns, 1842), una especie que se distribuye desde San Diego, CA hasta Perú. Las especies del género Sphoeroides poseen un cuerpo alargado, grueso, robusto y relativamente suave. Su nombre común se debe a los patrones característicos en la piel de la especie que simulan un blanco de tiro (dianas) (Thomson et al. 2000). Se localizan en la zona costera, principalmente en zonas protegidas, estuarios, lagunas costeras y otras áreas salobres; así como sobre corales y en interfaces rocoso arenosas. Es una especie fecunda que se reproduce a finales de la primavera en zonas cercanas a la costa (Amezcua-Linares 1996). Son preferentemente carnívoros y contienen una toxina llamada tetrodotoxina, que se encuentra principalmente en el hígado (Mallard et al.

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“…The digestive tract development and capacity for digestion of food in S. annulatus from fertilized egg to 32 DPH were studied by histological, histochemical and reverse-transcription polymerase chain reaction methods (García-Gasca et al 2006 species from the Tetraodontidae family (Kumai et al 1989). As for the activity of digestive enzymes during larval development in the bullseye puffer, it was found that trypsin activity, one of the main digestive enzymes during the larval phase of marine fish, starts at 2 DHP reaching a peak from 16 to 24 DPH, which corresponds to the Artemia feeding period (García-Gasca et al 2006). The gene expression of trypsinogen was first detected in 24-h fertilized eggs, then showing a significant increase at 75 h post-fertilization and at 4 DPH when the fish larvae starts exogenous feeding.…”

Section: Ontogeny Of Digestive Tract and Enzyme Expression And Activitymentioning

confidence: 99%

“…High protein requirements are typical for a carnivorous fish. However, S. annulatus and other puffer fish species do not have a stomach during their life cycle (Kumai et al 1989;García-Gasca et al 2006). Thus, it remains to be elucidated how dietary protein digestion in this stomachless fish is carried out.…”

Section: Nutrient Requirementsmentioning

confidence: 99%

Nutrition and feeding research in the spotted rose snapper (Lutjanus guttatus) and bullseye puffer (Sphoeroides annulatus), new species for marine aquaculture

García‐Ortega

2008

Fish Physiol Biochem

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The spotted rose snapper (Lutjanus guttatus) and bullseye puffer (Sphoeroides annulatus) are fish species from the tropical Eastern Pacific for which controlled production of larvae and juveniles has been accomplished in recent years. Diverse topics relating to their biology and aquaculture production are currently under study, in particular the nutrition and feeding aspects required to formulate practical feeds and rearing protocols. Improvements in larval growth and survival are possible by feeding live food organisms with natural or enhanced essential fatty acids content and highly digestible artificial microdiets. The ontogeny of the digestive tract and the expression and activity of digestive enzymes have been described for S. annulatus larvae. The effect of various protein and lipid levels on growth and feed utilization has been studied in juvenile and on-growing fish. Both species have carnivorous feeding habits and require high levels of protein in their diets, from 40% to 45% (dry weight) in spotted rose snapper and above 50% in bullseye puffer, with the younger stages requiring the highest protein levels. Encouraging results have been obtained in feeding experiments with different sources of dietary protein from animal and plant origin to evaluate their suitability as feed ingredients in practical diets. Optimization of fish culture practices through feeding management has also been investigated. Trials with various fish densities and feeding frequencies in intensive culture systems are providing information to improve feed utilization and growth in on-growing fish. Further research is underway to evaluate factors in broodstock nutrition which have an impact on egg and larval quality, and into the use of various commercially available oil sources in on-growing diets. In this paper, the results on nutrition and feeding research with both species are reviewed and research needs to support their commercial production in the region are discussed.

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Development of the digestive tract, trypsin activity and gene expression in eggs and larvae of the bullseye puffer fish Sphoeroides annulatus

Cited by 74 publications

References 25 publications

Digestive system development and study of acid and alkaline protease digestive capacities using biochemical and molecular approaches in totoaba (Totoaba macdonaldi) larvae

Digestive system development and study of acid and alkaline protease digestive capacities using biochemical and molecular approaches in totoaba (Totoaba macdonaldi) larvae

Biotecnología para la producción masiva de juveniles del botete diana Sphoeroides annulatus: inducción hormonal y cultivo larvario

Nutrition and feeding research in the spotted rose snapper (Lutjanus guttatus) and bullseye puffer (Sphoeroides annulatus), new species for marine aquaculture

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