Development of tissue-engineered vascular grafts from decellularized parsley stems

Cevik, Merve; Dikici, Serkan

doi:10.1039/d3sm01236k

Cited by 6 publications

(2 citation statements)

References 106 publications

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“…The samples were gold sputter-coated at 15 kV for 2.5 min to increase the conductivity. An FEI Inspect F SEM (Philips/FEI XL-20 SEM, Cambridge, UK) was used with 10 kV power. − …”

Section: Methodsmentioning

confidence: 99%

Quantitative Evaluation of the Pore and Window Sizes of Tissue Engineering Scaffolds on Scanning Electron Microscope Images Using Deep Learning

Karaca,

Aldemir Dikici

2024

ACS Omega

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The morphological characteristics of tissue engineering scaffolds, such as pore and window diameters, are crucial, as they directly impact cell-material interactions, attachment, spreading, infiltration of the cells, degradation rate and the mechanical properties of the scaffolds. Scanning electron microscopy (SEM) is one of the most commonly used techniques for characterizing the microarchitecture of tissue engineering scaffolds due to its advantages, such as being easily accessible and having a short examination time. However, SEM images provide qualitative data that need to be manually measured using software such as ImageJ to quantify the morphological features of the scaffolds. As it is not practical to measure each pore/window in the SEM images as it requires extensive time and effort, only the number of pores/windows is measured and assumed to represent the whole sample, which may cause user bias. Additionally, depending on the number of samples and groups, a study may require measuring thousands of samples and the human error rate may increase. To overcome such problems, in this study, a deep learning model (Pore D2) was developed to quantify the morphological features (such as the pore size and window size) of the open-porous scaffolds automatically for the first time. The developed algorithm was tested on emulsion-templated scaffolds fabricated under different fabrication conditions, such as changing mixing speed, temperature, and surfactant concentration, which resulted in scaffolds with various morphologies. Along with the developed model, blind manual measurements were taken, and the results showed that the developed tool is capable of quantifying pore and window sizes with a high accuracy. Quantifying the morphological features of scaffolds fabricated under different circumstances and controlling these features enable us to engineer tissue engineering scaffolds precisely for specific applications. Pore D2, an open-source software, is available for everyone at the following link: .

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“…The samples were gold sputter-coated at 15 kV for 2.5 min to increase the conductivity. An FEI Inspect F SEM (Philips/FEI XL-20 SEM, Cambridge, UK) was used with 10 kV power. − …”

Section: Methodsmentioning

confidence: 99%

Quantitative Evaluation of the Pore and Window Sizes of Tissue Engineering Scaffolds on Scanning Electron Microscope Images Using Deep Learning

Karaca,

Aldemir Dikici

2024

ACS Omega

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“…High biocompatibility and biodegradation are the unique advantages of decellularized biomaterials to substitute synthetic materials for scaffold manufacturing (Singh et al, 2023). Cevik and Dikici (2024) fabricated the tubular scaffolds from decellularized parsley stems, which were used to be tissue-engineered vascular grafts after recellularization. The biocompatible F I G U R E 6 Main procedures of cell-cultured meat manufacturing (a) (Thyden et al, 2022) and the morphology of decellularized plants (b) (Lee et al, 2019).…”

Section: Cell-cultured Meatmentioning

confidence: 99%

Progress of plant‐derived non‐starch polysaccharides and their challenges and applications in future foods

Guo,

Zhang,

Mujumdar

2024

Comp Rev Food Sci Food Safe

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The development of future food is devoted not only to obtaining a sustainable food supply but also to providing high‐quality foods for humans. Plant‐derived non‐starch polysaccharides (PNPs) are widely available, biocompatible, and nontoxic and have been largely applied to the food industry owing to their mechanical properties and biological activities. PNPs are considered excellent biomaterials and food ingredients contributing to future food development. However, a comprehensive review of the potential applications of PNPs in future food has not been reported. This review summarized the physicochemical and biological activities of PNPs and then discussed the structure–activity relationships of PNPs. Latest studies of PNPs on future foods including cell‐cultured meat, food for special medical purposes (FSMPs), and three‐dimensional‐printed foods were reviewed. The challenges and prospects of PNPs applied to future food were critically proposed. PNPs with strong thermal stability are considered good thickeners, emulsifiers, and gelatinizers that greatly improve the processing adaptability of foods. The mechanical properties of PNPs and decellularized plant‐based PNPs make them desirable scaffolds for cultured meat manufacturing. In addition, the biological activities of PNPs exhibit multiple health‐promoting effects; therefore, PNPs can act as food ingredients producing FSMP to promote human health. Three‐dimensional printing technology enhances food structures and biological activities of functional foods, which is in favor of expanding the application scopes of PNPs in future food. PNPs are promising in future food manufacturing, and more efforts need to be made to realize their commercial applications.

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Toward “Green” Vessels: Characterization of Microstructure, Mechanics, and Endothelial Cell Interaction on Three Macro‐Tubular Plants for Vascular Tissue Engineering Applications

Salehi,

Ernez,

Salido

et al. 2024

Adv Materials Technologies

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Vascular tissue engineering aims to create vessel models for in vitro research and develop vascular grafts for in vivo applications using tubular scaffolds. Natural scaffolds outperform synthetic ones due to their biocompatibility and natural microenvironment supporting cell growth. Given the importance of producing biocompatible tubular scaffolds through cost‐effective and uncomplicated processes, this study introduces nature‐derived tubular structures from three decellularized tubular plants (Water Spinach, Green Onion, and Water Horsetail) as novel alternatives. Microstructural characterization on the luminal surfaces of the plants reveals unique surface topography for each. Water Spinach is the most promising graft candidate in suturability tests besides presenting the highest elongation before rupture in tensile test. Assessment of human endothelial cells on the luminal surfaces of decellularized scaffolds shows higher expression of Ki‐67 protein and a consistent increase in cell number on water spinach and green onion scaffolds compared to tissue culture plate as a control. Focal adhesion‐related molecule Vinculin is expressed more than twice on all scaffolds compared to control, and confluent cell monolayers are formed on water spinach and green onion scaffolds, as confirmed by VE‐cadherin. This study proposes an innovative approach to use the natural structure of macro‐tubular plants for the preparation of vascular scaffolds.

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Development of tissue-engineered vascular grafts from decellularized parsley stems

Abstract: Decellularized parsley stems: A novel tubular scaffold for developing tissue-engineered vascular grafts.

Cited by 6 publications

References 106 publications

Quantitative Evaluation of the Pore and Window Sizes of Tissue Engineering Scaffolds on Scanning Electron Microscope Images Using Deep Learning

Quantitative Evaluation of the Pore and Window Sizes of Tissue Engineering Scaffolds on Scanning Electron Microscope Images Using Deep Learning

Progress of plant‐derived non‐starch polysaccharides and their challenges and applications in future foods

Toward “Green” Vessels: Characterization of Microstructure, Mechanics, and Endothelial Cell Interaction on Three Macro‐Tubular Plants for Vascular Tissue Engineering Applications

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