Development of twelve polymorphic microsatellite markers in the edible cockle Cerastoderma edule (Bivalvia: Cardiidae)

Martínez, Luisa; Arias, Alberto; Méndez, Josefina; Insua, Ana; Freire, Ruth

doi:10.1007/s12686-009-9026-7

Cited by 11 publications

(8 citation statements)

References 10 publications

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“…Therefore, microsatellite loci showed high levels of polymorphism in P. nobilis, which is consistent with the number of alleles observed in other bivalve species (Martínez et al, 2009;Pereira et al, 2010). Significant heterozygote deficiency was detected in five loci (Table 2).…”

Section: Resultssupporting

confidence: 86%

Highly polymorphic microsatellite markers for the Mediterranean endemic fan mussel Pinna nobilis

et al. 2014

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Pinna nobilis is an endemic bivalve of the Mediterranean Sea whose populations have decreased in the last decades due to human pressure; as consequence, it was declared a protected species in 1992. Despite its conservation status, few genetic studies using mitochondrial markers have been published. We report on the isolation and development of 10 microsatellite loci for the fan mussel, Pinna nobilis. All loci (2 di-nucleotide, 5 tri-nucleotide, 2 tetra-nucleotide and 1 penta-nucleotide) are characterized by high polymorphism levels in 76 individuals tested from two populations in Balearic Islands (Spain, Western Mediterranean Sea). Number of alleles ranged from 4 to 24 and expected heterozygosity ranged from 0.4269 to 0.9400. These microsatellites could be very useful to assess the genetic diversity and connectivity patterns of P. nobilis and to establish new conservation strategies.

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Section: Resultssupporting

confidence: 86%

Highly polymorphic microsatellite markers for the Mediterranean endemic fan mussel Pinna nobilis

et al. 2014

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“…In general, similar values of genetic diversity were found in adults and post‐larvae of P. nobilis from the different localities. The heterozygosity values are similar to those recorded on other mollusc species from the Mediterranean Sea, such as Cerastoderma glaucum (Martínez, Arias, Mendez, Insua, & Freire, ), Mytilus galloprovincialis (Li, Liang, Sui, Gang‐Go, & He, ), Sepia officinalis (Perez‐Losada, Guerra, Carvalho, Sanjuan, & Shaw, ), Octopus vulgaris (Casu et al, ) and Loligo vulgaris (Guarniero et al, ).…”

Section: Discussionsupporting

confidence: 82%

Gene pool and connectivity patterns of Pinna nobilis in the Balearic Islands (Spain, Western Mediterranean Sea): Implications for its conservation through restocking

González‐Wangüemert

Basso

Balau

et al. 2018

Aquatic Conservation

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1. Pinna nobilis is an endemic bivalve of the Mediterranean Sea, and a vulnerable species registered as endangered and protected under the European Council Directive 92/43/EEC and Barcelona Convention. 2. In early autumn 2016, a mass mortality event impacted P. nobilis populations in the south-western Mediterranean Sea, including the Balearic Islands. At the time of this study, P. nobilis still maintained high population densities along the Balearic coasts (Western Mediterranean).3. This study evaluated the connectivity of P. nobilis post-larvae and adults in seagrass habitats around the Balearic Islands and identified its source and sink populations.These objectives were reached through a multidisciplinary approach including population genetics (10 microsatellites) and hydrodynamic modelling. 4. High genetic diversity was found and significant genetic differentiation (inferred by fixation index F ST ) was detected between post-larvae samples, but not between adult populations. Significant genic and genotypic differentiation was recorded for adults and post-larvae.5. This pattern was confirmed by correspondence analysis using allele frequencies.The genetic connectivity pattern was consistent with marine currents and dispersal models.6. This work not only improves knowledge of the P. nobilis gene pool in south-west Mediterranean populations and their connectivity patterns, but is also crucial to help evaluate the possibility of recovery from source populations and the possibility of restocking programmes, as well as provide a solid base to establish effective marine reserve networks.

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“…Microsatellites were amplified using primers for 12 loci, reported previously to be polymorphic in cockle ( C. edule ) populations 15 . Of the 12 primer pairs, 9 pairs amplified products in all cockle DNA samples, with 1–2 alleles observed in normal samples and 1–4 neoplastic alleles observed.…”

Section: Methodsmentioning

confidence: 99%

“…2a) with high (>75%) or moderate (15–75%) amounts of neoplastic cells in the hemolymph. Nine polymorphic microsatellite loci 15 were amplified from normal animals, and allele sizes from tissue and hemocytes of each normal animal all matched, but allele sizes in hemocytes and tissue of diseased animals were discordant (Fig. 2b, c).…”

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confidence: 99%

Widespread transmission of independent cancer lineages within multiple bivalve species

Metzger

Villalba

Carballal

et al. 2016

Nature

202

270

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Most cancers arise from oncogenic changes in the genomes of somatic cells, and while the cells may migrate by metastasis, they remain within that single individual. Natural transmission of cancer cells from one individual to another has been observed in two distinctive cases in mammals (Tasmanian devils1 and dogs2,3), but these are generally considered to be rare exceptions in nature. The discovery of transmissible cancer in soft-shell clams (Mya arenaria)4 suggested that this phenomenon might be more widespread. Here we analyzed disseminated neoplasia in mussels (Mytilus trossulus), cockles (Cerastoderma edule), and golden carpet shell clams (Polititapes aureus) and found that neoplasias in all three species are attributable to independent transmissible cancer lineages. In mussels and cockles, the cancer lineages are derived from their respective host species, but unexpectedly, cancer cells in P. aureus are all derived from Venerupis corrugata, a different species living in the same geographic area. No cases of disseminated neoplasia have thus far been found in V. corrugata from the same region. These findings show that transmission of cancer cells in the marine environment is common in multiple species, that it has originated many times, and that while most transmissible cancers were found spreading within the species of origin, cross-species transmission of cancer cells can occur.

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Development of twelve polymorphic microsatellite markers in the edible cockle Cerastoderma edule (Bivalvia: Cardiidae)

Cited by 11 publications

References 10 publications

Highly polymorphic microsatellite markers for the Mediterranean endemic fan mussel Pinna nobilis

Highly polymorphic microsatellite markers for the Mediterranean endemic fan mussel Pinna nobilis

Gene pool and connectivity patterns of Pinna nobilis in the Balearic Islands (Spain, Western Mediterranean Sea): Implications for its conservation through restocking

Widespread transmission of independent cancer lineages within multiple bivalve species

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