Development of two brain cell lines from goldfish and silver crucian carp and viral susceptibility to Cyprinid herpesivirus-2

Xu, Ye; Zhou, Yi; Wang, Fengzhi; Chao, Ding; Cao, Jie; Duan, Hongan

doi:10.1007/s11626-019-00402-y

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…Xu et al . (2019) demonstrated CPE in two newly developed brain cell lines CrCB and GFB from 3d after infection with CyHV‐2 in silver crucian carp and goldfish, respectively. The cell line GiCF may serve as an ideal infection platform to characterize the apoptosis effect of CyHV‐2.…”

Section: Diagnosis Of Cyhv‐2 Infectionmentioning

confidence: 99%

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Thangaraj

Nithianantham

Dharmaratnam

et al. 2020

Reviews in Aquaculture

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Cyprinid herpesvirus-2 (CyHV-2) is a linear double-stranded DNA virus in the genus Cyprinivirus of family Alloherpesviridae. The virus is known to be highly pathogenic to ornamental goldfish (Carassius auratus), crucian carp (C. carassius) and Gibel carp (C. auratus gibelio), and also to the hybrids of goldfish and other carps. Cyprinid herpesvirus-2, having the smallest genome (290.3 kb) among Cyprinivirus, causes herpesviral hematopoietic necrosis disease (HVHND) that results in huge economic losses in aquaculture industry as the disease can cause high mortality (50-100%) among the affected fish. The disease was initially reported as the cause of epizootics in juvenile goldfish of Japan during 1992 and 1993. To date, this disease has been reported around the world including Europe, North America, Oceania and Asia. Huge economic losses due to the CyHV-2 infection among cultured gibel carp in China, during 2011-2012, mass mortality in crucian carp during 2012 in Italy, 95% mortality in goldfish during 2014 in France, 85% mortality in goldfish during 2016 in Poland had been reported. Strategies for controlling the spread of CyHV-2 are thus urgently required to limit economic damage. Furthermore, the review will shed light on lacunae in current knowledge as well as on the perspectives that merits further investigations on CyHV-2 research. The paper forms the first comprehensive overview of CyHV-2 causing a serious economically significant fish disease and, will be helpful for the researchers to get all related information from a single manuscript.

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Section: Diagnosis Of Cyhv‐2 Infectionmentioning

confidence: 99%

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Thangaraj

Nithianantham

Dharmaratnam

et al. 2020

Reviews in Aquaculture

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“…In the present study, the plating efficiency was estimated high at 1000 cell per flask whereas, low cell proliferation was observed in 100 cell per flask. The SPB cell line has high plating efficiency, similar to fin cell line of Pangasianodon hypophthalmus (Soni et al., 2018), gill cell line of P. hypophthalmus (Sathiyanarayanan et al., 2022) and silver crucian carp and goldfish brain cells (Xu et al., 2019). SPB cell lines have a doubling time of roughly 26 h. The doubling time of cells in established cell lines is an important element since it is dependent on it for a variety of applications, including gene expression research to make recombinant proteins.…”

Section: Discussionmentioning

confidence: 99%

Development and characterization of brain cell line from Trachinotus blochii and its application in virological and gene expression studies

Nafeez Ahmed,

Mithra,

Suryakodi

et al. 2024

Journal of Fish Diseases

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A permanent cell line, SPB (Snubnose pompano brain) was established from Trachinotus blochii by the explant culture method. It has been sub‐cultured more than 75 passages and showed optimal growth at 28°C using L‐15 medium supplemented with 15% to 20% FBS. The SPB cells were cryopreserved at different passage levels for various applications. SPB cells were composed of fibroblastic and epithelial‐like cells. The SPB cells were tested for mycoplasma contamination which was found to be negative. The origin of the SPB cell line from T. blochii was confirmed by amplification of the mitochondrial cytochrome oxidase I (COI) gene. The transfection efficiency of SPB cell line is 15% assessed by expression of green fluorescent protein using pEGFP‐N1 plasmid. In addition, two CMV promotor plasmids pFNCPE42‐DNA and pcDNAVP28 were transfected to SPB cells and it shows high expression levels of FNCP of fish nodavirus and VP28 protein of white spot syndrome virus by immunostaining. The SPB cells showed susceptibility to SJNNV and the infection was confirmed by RT‐PCR, Western blot, ELISA, TCID50 and RT‐qPCR. Experimental infection was carried out in T. blochii using SJNNV propagated in SPB cell line and found 100% mortality with clinical signs. The infection was confirmed by RT‐PCR. The SPB cell line can be used for propagation of fish viral pathogens and production of the recombinant proteins.

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“…The PCR-positive homogenate was selected, centrifuged at 4000×g for 15 min, the supernatant was filtered through a 0.45μm sterile filter, and 1 mL/bottle was inoculated onto crucian carp brain cells (CrCB) or goldfish brain cells (GFB) (Y. Xu et al, 2019) over 80% fluent monolayer. Cells were observed daily for growth status and CPE.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a Cyprinid herpesvirus strain YZ01 from apparently healthy goldfish after rising water temperature

Wang

Zhou

et al. 2021

Preprint

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A Cyprinid herpesvirus 2 (CyHV-2) strain YZ01 was isolated from an apparently healthy goldfish with history of exposure to CyHV-2 when the water temperature was heated from 3℃ to 25 ℃.Brain, kidney, spleen (referred to as BKS) and intestine of sick and dead fish as well as crucian carp (CrCB) or goldfish brain cell (GFB) suspensions inoculated with above tissue homogenate were collected for PCR assay.Infected cell layers were prepared into ultrathin sections for transmission electron microscopy. Virus DNA were purified from cell suspensions for whole genome sequencing and analysis. Intestine and mixed brain, kidney and spleen fluid (BKS) of dead goldfish and BKS-infected CrCB suspensions as well as their subsequent passages were tested positive by PCR. Nucleus deformation, lattice-like arrangement of inclusion bodies and various forms of immature and mature virions in the nucleus, cytoplasm and outside of cell membranes were observed. Whole genome analysis of 7 CyHV-2 strains available in GeneBank showed that all strains genomes share very high homology while YZ01 was more close to CyHV-2 CNDF-TB2015 and SY-C1 which are classified as C genotype.

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Development of two brain cell lines from goldfish and silver crucian carp and viral susceptibility to Cyprinid herpesivirus-2

Cited by 12 publications

References 30 publications

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

Development and characterization of brain cell line from Trachinotus blochii and its application in virological and gene expression studies

Isolation and characterization of a Cyprinid herpesvirus strain YZ01 from apparently healthy goldfish after rising water temperature

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