Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds

Han, En‐Hou; Yang, Yi; He, Zheng; Cai, Jun; Zhang, Xinai; Dong, Xiaopeng

doi:10.1016/j.ab.2015.07.001

Cited by 57 publications

(18 citation statements)

References 37 publications

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“…Figure 7a demonstrates the relationship between the cathodic and anodic peak potentials (E c P and E a P ) and a log ν for the biosensor in the solution comprising of 1.0 × 10 −1 M phosphate buffer (pH 5) and 1.0 × 10 −3 M of catechol. For this biosensor, the peak potentials can be exhibited by Laviron [42] equations (1) and (2):…”

Section: Voltammetric Responsementioning

confidence: 99%

“…The health of organisms is affected by phenolic compounds. These compounds release into ground and surface waters is occurred due to their utilization in product fabrication among oil refining, coal conversion, medicines, surfactants, pesticides, colourants, plastics and resin formation [1][2][3]. European Community announced that the maximum amount of phenols in effluent should be <1 ppm [4].…”

Section: Introductionmentioning

confidence: 99%

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Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

et al. 2019

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In this study, a new biosensor is developed with reliable and easy-to-use biodevice properties for catechol determination in real samples. A method is proposed for the fabrication of biosensors to sense catechol based on the adsorption method of laccase immobilization. Hence, a glassy carbon electrode was modified via graphene oxide nanosheets and then it was modified with a gold-cetyltrimethylammonium bromide nanocomposite to adsorb and immobilize laccase on the electrode surface. The results showed laccase immobilization onto the reformed glassy carbon electrode, and a direct electron transfer reaction between laccase and the electrode. The mechanism of electron transferring was EC. Also, k s and α were calculated as 0.41 s −1 and 0.33, respectively. For this biosensor two linear ranges, 0.1 × 10 −6 to 5 × 10 −6 M and 16.7 × 10 −6 to 166 × 10 −6 M, and a detection limit of 1.5 × 10 −6 M were obtained.

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Section: Voltammetric Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

et al. 2019

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“…These nano materials further enhance the surface coverage as well as sensitivity resulting in low detection limits and low sample requirements [32]. A wide range of nanomaterials such as metal nanoparticles [33][34][35], metal oxide nanostructures [36][37][38][39][40][41][42][43][44][45][46], polymeric nanoparticles [47], carbon nanotubes [48][49][50][51], graphene [52][53][54][55][56], quantum dots [57][58][59][60][61], hydrogels [62][63][64][65][66], and ceramic nanostructures [67,68] have been extensively studied as interface materials in electrochemical nanosensors. The nano-dimensional interface in electrochemical sensors exhibits several unique characteristics.…”

Section: Evolution Of Electrochemical Biosensorsmentioning

confidence: 99%

Metabolic Syndrome—An Emerging Constellation of Risk Factors: Electrochemical Detection Strategies

Madhurantakam

Devi

Babu

et al. 2019

Sensors

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Metabolic syndrome is a condition that results from dysfunction of different metabolic pathways leading to increased risk of disorders such as hyperglycemia, atherosclerosis, cardiovascular diseases, cancer, neurodegenerative disorders etc. As this condition cannot be diagnosed based on a single marker, multiple markers need to be detected and quantified to assess the risk facing an individual of metabolic syndrome. In this context, chemical- and bio-sensors capable of detecting multiple analytes may provide an appropriate diagnostic strategy. Research in this field has resulted in the evolution of sensors from the first generation to a fourth generation of ‘smart’ sensors. A shift in the sensing paradigm involving the sensing element and transduction strategy has also resulted in remarkable advancements in biomedical diagnostics particularly in terms of higher sensitivity and selectivity towards analyte molecule and rapid response time. This review encapsulates the significant advancements reported so far in the field of sensors developed for biomarkers of metabolic syndrome.

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“…There have been extensive research works for developing tyrosinase (Tyr) based electrochemical sensor in view of its simplicity, efficiency and easy operation in field applications. The Tyr is a metalloprotein (EC 1.14.18.1) containing a binuclear copper active that can first catalyze phenol to catechol, and then catechol is converted to o-benzoquinone in the presence of oxygen [10][11][12]. Hence, the most of Tyr based electrochemical sensors are based on the determination of electrochemical reduction of o-benzoquinone [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Amplified Electrochemical Response of Phenol by Oxygenation of Tyrosinase Coupling with Electrochemical‐chemical‐chemical Redox Cycle

Guo

Zhao

et al. 2019

Electroanalysis

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In this work, an novel electrochemical‐chemical‐chemical (ECC) redox cycle was designed in an enzyme‐based sensor for acquiring additional signal amplification. The tyrosinase (Tyr) was entrapped in a sulfonated polyaniline−chitosan (SPAN−CS) composite which was used as a redox capacitor on a glass carbon electrode. Firstly, the substrate, phenol was catalyzed to catechol and further catalyzed to o‐benzoquinone by Tyr. Next, in the presence of Ru(NH3)6Cl2, the reduced state of SPAN(SPANred) was reacted with o‐benzoquinone to form it's oxidized state (SPANox) and catechol, then SPANox was reduced back to SPANred by Ru(II) in the solution. Finally, the amplified anodic current of catechol was obtained on electrode through above ECC redox cycle system. In addition, the ECC redox cycling led to a high signal‐to‐background ratio. The voltammetric response showed excellent analytical performance to phenol over two linear range of 3.5 to 200.0 nmol L−1 and 200.0 to 2000.0 nmol L−1 with a high sensitivity of 2204 μA mM−1. The detection limit was obtained to be 0.8 nmol L−1 (S/N=3). Furthermore, the proposed approach exhibited good repeatability, stability and specificity, and could offer practicality in the detection of phenol in tap water.

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Development of tyrosinase biosensor based on quantum dots/chitosan nanocomposite for detection of phenolic compounds

Cited by 57 publications

References 37 publications

Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

Laccase immobilized onto graphene oxide nanosheets and electrodeposited gold–cetyltrimethylammonium bromide complex to fabricate a novel catechol biosensor

Metabolic Syndrome—An Emerging Constellation of Risk Factors: Electrochemical Detection Strategies

Amplified Electrochemical Response of Phenol by Oxygenation of Tyrosinase Coupling with Electrochemical‐chemical‐chemical Redox Cycle

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