2018
DOI: 10.1016/j.jneumeth.2018.05.025
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Development of ultra-thin chips for immobilization of Caenorhabditis elegans in microfluidic channels during irradiation and selection of buffer solution to prevent dehydration

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Cited by 14 publications
(30 citation statements)
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“…We investigated the effects of targeted microbeam irradiation using carbon ion ( 12 C 6+ ) particles accelerated by an azimuthally varying field cyclotron installed at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) facility of QST-Takasaki. Irradiation generally followed previously described methods [16,18]. We delivered targeted microbeam irradiation using a collimating microbeam system [18].…”
Section: Evaluation Of Carbon-ion Collimationmentioning
confidence: 99%
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“…We investigated the effects of targeted microbeam irradiation using carbon ion ( 12 C 6+ ) particles accelerated by an azimuthally varying field cyclotron installed at the Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) facility of QST-Takasaki. Irradiation generally followed previously described methods [16,18]. We delivered targeted microbeam irradiation using a collimating microbeam system [18].…”
Section: Evaluation Of Carbon-ion Collimationmentioning
confidence: 99%
“…Before biological experiments using C. elegans, we evaluated the distribution of the collimated carbon-ion beam using a micro-aperture with a 60-µm and 20-µm diameter. We irradiated an ion-track detector CR-39 film (Solid State Nuclear Track Detector HARZLAS TNF-1, Fukuvi Chemical Industry, Fukui, Japan) with 10 or 100 ion particles and observed the resulting etch pits as described previously [10,16].…”
Section: Evaluation Of Carbon-ion Collimationmentioning
confidence: 99%
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“…We identified a suitable buffer solution to maintain good worm conditions in the channel. Using this method, irradiating a specific region of C. elegans with the exact number of heavy ions is now possible as well as analyzing the immediate response on locomotive activity [23][24][25].…”
Section: Manual Targeting Irradiationmentioning
confidence: 99%