Development of Urease/Layered Double Hydroxides Nanohybrid Materials for the Urea Detection: Synthesis, Analytical and Catalytic Characterizations

Baccar, Z. M.; Caballero, David; Zine, Nadia; Jaffrézic‐Renault, Nicole; Errachid, A.

doi:10.1166/sl.2009.1130

Cited by 6 publications

(7 citation statements)

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“…They are also particularly welladapted to the design of electrochemical biosensors [31]- [33]. Applications of this kind of material have been already proposed in the case of antibodies and enzymes [36]- [39]. In the case of tyrosinase, hybrid materials made of chitosan and LDHs were described [40].…”

Section: Introductionmentioning

confidence: 99%

Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

et al. 2017

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Abstract-Gold screen-printed electrodes coated with thin films of layered double hydroxides containing cobalt and aluminium (Co 1.57 Al(OH) x SO 4 , shortened as CoAl) have been investigated for the design of an electrochemical tyrosinasebased biosensor used for the detection of a complex mixture of polyphenols extracted from green tea. Physicochemical analyses show that the resulted biosensor exhibits very attractive characteristics: a high sensitivity, a large dynamic range (up to 1000 ng.mL −1 ), and very low limits of detection (0.33 pg.mL −1 and 0.03 pg.mL −1 for oxidation and reduction, respectively).Index Terms-Atomic force microscopy, electrochemical biosensor, layered double hydroxide, polyphenols, tyrosinase.

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Section: Introductionmentioning

confidence: 99%

Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

et al. 2017

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“…In fact, due to their particular structure (consisting of positively charged cation layers with charge balancing anions and water molecules inserted between these layers), inorganic LDH matrixes have presented specific properties, such as their high ion exchange capacity, their encapsulation ability and their biocompatibility [3]. Many applications have been proposed, based on the immobilization of different enzymes into LDH like urease [4], cholesterol oxidase [5] and lactate dehydrogenase [6].…”

Section: Introductionmentioning

confidence: 99%

Hydrocalumite Thin Films for Polyphenol Biosensor Elaboration

Soussou

Gammoudi

Kalboussi

et al. 2017

IEEE Trans.on Nanobioscience

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Hybrid thin films based on Hydrocalumite (CaAlCl layered double hydroxide LDH) and tyrosinaseenzyme have been used for the elaboration of a high sensitive amperometric biosensor detecting polyphenols extracted from green tea. Structural properties of LDH nanomaterials were characterized by X-ray powder diffraction and Infra-Red spectroscopy, confirming its crystalline phase and chemical composition. CaAlCl-LDHs-thin films were deposited by spin-coating, and studied by atomic force microscopy to obtain information about the surface morphology of this host matrix before and after enzyme's immobilization. Electrochemical study using cyclic voltammetry and chronoamperometry shows good performances of the built-in biosensor with a high sensitivity for polyphenols concentrations ranging from 24 pM to and a limit of detection of 1.2 pM.

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“…The immobilization process is based on the ability of the hydroxyl, amine or thiols groups of the biomolecules to cross link with of the metals oxide carrier of the LDHs producing covalent links. The enzymes immobilized onto LDH show high activity and good performance stability [17,[19][20].…”

Section: Introductionmentioning

confidence: 99%

“…A high sensitivity of the impedimetric biosensor to the hybridization of the 80-bases long complementary ss oligonucleotide sequence was obtained with a response of 286.7 and 238.5 Ω /ng. [14,18,19,26]. We mixed small amounts (approx.…”

Section: Introductionmentioning

confidence: 99%

Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences

Baccar¹,

Caballero

Eritja

et al. 2012

Electrochimica Acta

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International audienceDNA testing requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. In this paper, a simple and label-free DNA-biosensor has been investigated based on the detection of DNA hybridization on layered double hydroxide (LDH) nanomaterials with special emphasis on targeting long single stranded DNA sequences. First, the immobilization of a 20 bases long DNA probe on a thin layer of Mg2AlCO3 and Mg3AlCO3 LDH was studied. Then, DNA hybridization reaction was detected by means of Electrochemical Impedance Spectroscopy. The resulting biosensor showed a high sensitivity for the detection of 80 bases long DNA complementary sequences. The dynamic range was 18-270 ng/ml with a detection limit lower than 1.8 ng/ml

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Development of Urease/Layered Double Hydroxides Nanohybrid Materials for the Urea Detection: Synthesis, Analytical and Catalytic Characterizations

Cited by 6 publications

References 0 publications

Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

Hydrocalumite Thin Films for Polyphenol Biosensor Elaboration

Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences

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