Development, Optimization, and Validation of an Ultrasound-Assisted Liquid–Liquid Microextraction (UALLME) for Selenomethionine Analyses in Cashew Nut (Anacardium occidentale) by Ultra-Performance Liquid Chromatography Coupled to Electrospray Ionization/Single Quadrupole Mass Spectrometer (UPLC-ESI/QDa)

Alcântara, Daniel B.; Riceli, Paulo; Almeida, Ana Debora S.; Luz, Lícia R.; Nascimento, Hélio Oliveira do; Fernandes, Tatiana Sainara Maia; Dionísio, Ana Paula; Castro, Ana Cecília R.; Nascimento, Ronaldo Ferreira do; Lopes, Gisele S.; Zocolo, Guilherme Julião

doi:10.1007/s12161-022-02355-7

Cited by 3 publications

(1 citation statement)

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“…The data were processed using Waters Empower™ 3 software (Waters ® Corp., Milford, MA, USA). Total ion chromatogram (TIC) mass scan acquisition was set to 50–1250 Da and a selected ion recording (SIR) for each one targeted mass was obtained [ 15 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

LC-MS Fingerprinting Development for Standardized Precipitate from Agastache mexicana, Which Induces Antihypertensive Effect through NO Production and Calcium Channel Blockade

Cruz-Torres,

Estrada-Soto,

Arias-Durán

et al. 2023

Pharmaceutics

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The aim of this work was to evaluate the vasorelaxant and antihypertensive effects of a standardized precipitate of the hydroalcoholic extract from Agastache mexicana (PPAm), comprising ursolic acid, oleanolic acid, acacetin, luteolin and tilianin, among others. In the ex vivo experiments, preincubation with L-NAME (nonspecific inhibitor of nitric oxide synthases) reduced the relaxation induced by PPAm; nevertheless, preincubation with indomethacin (nonspecific inhibitor of cyclooxygenases) did not generate any change in the vasorelaxation, and an opposed effect was observed to the contraction generated by CaCl2 addition. Oral administration of 100 mg/kg of PPAm induced a significant acute decrease in diastolic (DBP) and systolic (SBP) blood pressure in spontaneously hypertensive rats, without changes in heart rate. Additionally, PPAm showed a sustained antihypertensive subacute effect on both DBP and SBP for 10 days compared to the control group. On the other hand, human umbilical vein cells treated with 10 µg/mL of PPAm showed a significant reduction (p < 0.05) in intracellular adhesion molecule-1, compared to the control, but not on vascular cell adhesion molecule-1. In conclusion, PPAm induces a significant antihypertensive effect in acute- and subacute-period treatments, due to its direct vasorelaxant action on rat aortic rings through NO production and Ca2+ channel blockade.

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Section: Methodsmentioning

confidence: 99%