Development, standardization and assessment of PCR systems for purity testing of avian viral vaccines

Molecular detection and characterization of infectious laryngotracheitis virus (Gallid herpesvirus-1) from clinical samples of commercial poultry flocks in India

“…The primer pair targeting the envelope glycoprotein-G gene (US4 gene) of GaHV-1 as described by [25] viz. ILTp32 U2-CTA CGT GCT GGG CTC TAAT CC and ILTp32 L2-AAA CTC TCG GGT GGC TAC TGC were used in the PCR.…”

Molecular Characterization of Chicken Infectious Anemia Virus Isolated From Commercial Poultry with Respiratory Disease Complex in India

“…Viral DNA from tissue homogenate was extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) as per the manufacturer's instructions. The primer pair targeting the VP2 gene of CAV viz., CAV_P1-3'CTA AGA TCT GCA ACT GCG GA5' and CAV_P2 3'CCT TGG AAG CGG ATA GTC AT5' were used in the PCR detection to amplify a CAV virus specific 419-bp fragment (Ottiger, 2010). The amplification was carried out using PCR Master Mix (2X) (Fermentas, USA), with an initial denaturation at 95º C for 3 minutes, followed by 35 cycles at 94 °C for 1 min, 60 °C for 1 min, 72 ºC for 1 min, and a final extension at 72 °C for 10 min.…”

Isolation and phylogenetic characterization of haemagglutinin and neuraminidase genes of H9N2 low pathogenicity avian influenza virus isolated from commercial layers in India

“…The presence of AIV was confirmed by RT-PCR targeting of Matrix (M) gene [22]. Subtype identification was then determined by PCR, with the use of HA and NA genespecific primers [23,24] and sequencing.…”