Development, validation and application of an ICP-MS/MS method to quantify minerals and (ultra-)trace elements in human serum

Meyer, Sören; Markova, Mariya; Pohl, Gabriele; Marschall, Talke Anu; Pivovarova, O; Pfeiffer, Andreas F. H.; Schwerdtle, Tanja

doi:10.1016/j.jtemb.2018.05.012

Cited by 50 publications

(39 citation statements)

References 40 publications

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“…Zinc, iron, copper, manganese and selenium in commercial human serum samples were determined by an inductively coupled plasma mass spectrometry (ICP-MS/MS) system (Agilent ICP-QQQ-MS 8800, Waldbronn, Germany) using the method previously described in detail by Meyer et al [40].…”

Section: Methodsmentioning

confidence: 99%

A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum

Alker

Schwerdtle

Schomburg

et al. 2019

IJMS

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Zinc is an essential trace element, making it crucial to have a reliable biomarker for evaluating an individual’s zinc status. The total serum zinc concentration, which is presently the most commonly used biomarker, is not ideal for this purpose, but a superior alternative is still missing. The free zinc concentration, which describes the fraction of zinc that is only loosely bound and easily exchangeable, has been proposed for this purpose, as it reflects the highly bioavailable part of serum zinc. This report presents a fluorescence-based method for determining the free zinc concentration in human serum samples, using the fluorescent probe Zinpyr-1. The assay has been applied on 154 commercially obtained human serum samples. Measured free zinc concentrations ranged from 0.09 to 0.42 nM with a mean of 0.22 ± 0.05 nM. It did not correlate with age or the total serum concentrations of zinc, manganese, iron or selenium. A negative correlation between the concentration of free zinc and total copper has been seen for sera from females. In addition, the free zinc concentration in sera from females (0.21 ± 0.05 nM) was significantly lower than in males (0.23 ± 0.06 nM). The assay uses a sample volume of less than 10 µL, is rapid and cost-effective and allows us to address questions regarding factors influencing the free serum zinc concentration, its connection with the body’s zinc status, and its suitability as a future biomarker for an individual’s zinc status.

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Section: Methodsmentioning

confidence: 99%

A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum

Alker

Schwerdtle

Schomburg

et al. 2019

IJMS

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“…Solutions were analyzed via inductively coupled plasma tandem mass spectrometry (ICP-MS/MS) (8800 ICP-QQQ-MS, Agilent Technologies, Waldbronn, Germany) as described before. [27,28]…”

Section: Measurement Of Tes In Feces Serum and Tissuesmentioning

confidence: 99%

Effects of a Cumulative, Suboptimal Supply of Multiple Trace Elements in Mice: Trace Element Status, Genomic Stability, Inflammation, and Epigenetics

Finke

Winkelbeiner

Lossow

et al. 2020

Molecular Nutrition Food Res

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Scope: Trace element (TE) deficiencies often occur accumulated, as nutritional intake is inadequate for several TEs, concurrently. Therefore, the impact of a suboptimal supply of iron, zinc, copper, iodine, and selenium on the TE status, health parameters, epigenetics, and genomic stability in mice are studied. Methods and results: Male mice receive reduced or adequate amounts of TEs for 9 weeks. The TE status is analyzed mass-spectrometrically in serum and different tissues. Furthermore, gene and protein expression of TE biomarkers are assessed with focus on liver. Iron concentrations are most sensitive toward a reduced supply indicated by increased serum transferrin levels and altered hepatic expression of iron-related genes. Reduced TE supply results in smaller weight gain but higher spleen and heart weights. Additionally, inflammatory mediators in serum and liver are increased together with hepatic genomic instability. However, global DNA (hydroxy)methylation is unaffected by the TE modulation. Conclusion: Despite homeostatic regulation of most TEs in response to a low intake, this condition still has substantial effects on health parameters. It appears that the liver and immune system react particularly sensitive toward changes in TE intake. The reduced Fe status might be the primary driver for the observed effects.

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“…In order to examine dietary intake of essential minerals, their corresponding serum levels and the potentially beneficial or detrimental effects of these on birth outcomes in a South East Queensland cohort, this research has taken advantage of recently developed inductively coupled plasma mass spectrometry (ICP-MS) serum element extraction processes 16,17 and the Maternal Outcomes and Nutrition Tool (MONT). Based on previously validated food frequency questionnaires, the MONT collects self-reported data regarding 259 individual foods, micronutrient supplements, 190 constituent nutrients, their related components, and 8 birth outcomes.…”

Section: Introductionmentioning

confidence: 99%

Essential Mineral Intake During Pregnancy and Its Association With Maternal Health and Birth Outcomes in South East Queensland, Australia

McAlpine

McKeating

Vincze

et al. 2019

Nutr Metab Insights

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Micronutrient supplements are often recommended during pregnancy, yet their role and necessity remain poorly understood in the Australian population. This study aimed to determine the essential mineral intake of a population of pregnant women in South East Queensland and investigate the effects of supplements on their micronutrient status and birth outcomes. Women completing the Oral Glucose Tolerance Test at two South East Queensland hospitals between 180 and 210 days gestation provided fasting blood samples and dietary data using the Maternal Outcomes and Nutrition Tool (n = 127). Birth outcomes were sourced from medical records. Serum elemental profiles were determined by inductively coupled plasma mass spectrometry (ICP-MS) analysis. Intake of 8 essential minerals was compared with Australian dietary recommendations; matched serum mineral levels were compared with the current Queensland pregnancy reference ranges. Data were examined using cross-sectional cohort design and independent sample t-tests. Supplement use had no significant influence on serum values of trace elements or the incidence of hypertensive disorders, gestational diabetes, preterm birth or infant birthweight. Dietary selenium, zinc and iodine were significantly higher in women birthing beyond 41 completed weeks; selenium ( P = .026) and zinc ( P = .034) both made unique contributions to the regression models when controlling for confounders. Women exhibited adequate to excessive serum micronutrient levels compared with pregnancy reference ranges, a finding consistent with dietary intake calculations. Data suggest that excessive essential mineral intake contributed to prolonged pregnancy in this cohort, supporting previous studies in this population. Further research is required to determine individual needs and eliminate the potential for harm before recommending pregnancy supplements.

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Development, validation and application of an ICP-MS/MS method to quantify minerals and (ultra-)trace elements in human serum

Cited by 50 publications

References 40 publications

A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum

A Zinpyr-1-based Fluorimetric Microassay for Free Zinc in Human Serum

Effects of a Cumulative, Suboptimal Supply of Multiple Trace Elements in Mice: Trace Element Status, Genomic Stability, Inflammation, and Epigenetics

Essential Mineral Intake During Pregnancy and Its Association With Maternal Health and Birth Outcomes in South East Queensland, Australia

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