Development, validation and application of an ultra high performance liquid chromatographic-tandem mass spectrometric method for the simultaneous detection and quantification of five different classes of veterinary antibiotics in swine manure

Meersche, Tina Van den; Pamel, Els Van; Poucke, Christof Van; Herman, Lieve; Heyndrickx, Marc; Rasschaert, Geertrui; Daeseleire, Els

doi:10.1016/j.chroma.2015.12.046

Cited by 78 publications

(44 citation statements)

References 27 publications

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“…Van den Meersche et al (2016) describe a method using UHPLC/MS/MS for analysis of veterinary antibiotics extracted from swine manure. Analytes were extracted using a mixture of acetonitrile and trichloroacetic acid.…”

Section: Methodsmentioning

confidence: 99%

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Snow¹,

Cassada²,

Larsen³

et al. 2017

Water Environment Research

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A total of 79 papers published in 2016 were reviewed ranging from detailed descriptions of analytical methods, to fate and occurrence studies, to ecological effects and sampling techniques for a wide variety of emerging contaminants likely to occur in agricultural environments. New methods and studies on veterinary pharmaceuticals, steroids, antibiotic resistance genes, cyanotoxins, and potential effects of biochar use in agricultural environments continue to expand our knowledge base on the occurrence and potential impacts of these compounds. This review is divided into the following sections: Introduction, Analytical Methods, Steroid Hormones, Anthelmintics, Antibiotic Fate and Occurrence, Antibiotic Resistance Genes, Cyanotoxins, and Implications of Biochar in Agricultural Environments.

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Section: Methodsmentioning

confidence: 99%

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Snow¹,

Cassada²,

Larsen³

et al. 2017

Water Environment Research

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“…Details of the development and validation of the method were published elsewhere. [36] Additionally, a multicompound analysis for the detection and quantification of the selected antibiotics in different soil types will be developed. The second WP consists of the optimisation of existing qPCRs to quantify the most important antibiotic resistance genes.…”

Section: Methodsmentioning

confidence: 99%

“…sulfadiazine, trimethoprim and the detection of tylosin A in pig manure has been developed and validated according to the criteria of Commission Decision 2002/657/EC. [36] The method was already used to screen ten pig manure samples for the presence of the aforementioned antibiotics. In all samples, one or more antibiotics were detected with concentrations varying from very low (<10 μg/kg) to more than 40 000 μg/kg.…”

Section: Methodsmentioning

confidence: 99%

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Antibiotic use and resistance in animals: Belgian initiatives

Daeseleire¹,

Graef

Rasschaert³

et al. 2016

Drug Testing and Analysis

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The widespread use of antibiotics in animals is causing concerns about the growing risk for development and the spread of antibiotic-resistant bacteria. Antibiotic consumption is higher in animals than in humans as reported in a joint publication of EFSA (European Food Safety Agency), ECDC (European Centre for Disease Prevention and Control), and EMA (European Medicines Agency) using data from 2011 and 2012. Both in humans and animals, positive associations between the consumption of antibiotics and resistant bacteria are observed. Responsible use of antibiotics in humans and animals should therefore be promoted. In this paper some general aspects of antibiotic resistance such as microbiological versus clinical resistance, intrinsic versus acquired resistance, resistance mechanisms, and transfer of resistance are briefly introduced. In 2012, the Belgian Center of Expertise on Antimicrobial Consumption and Resistance in Animals (AMCRA) was founded. Its mission is to collect and analyze all data related to antibiotic use and resistance in animals in Belgium and to communicate these findings in a neutral and objective manner. One of AMCRA's 10 objectives is a 50% reduction in antibiotic consumption in veterinary medicine in Belgium by 2020. The aim of this paper is to report on the achievements of this national project. The Institute for Agricultural and Fisheries Research (ILVO, Merelbeke-Melle), in collaboration with Ghent University, is currently working on three nationally funded projects on antibiotic resistance in animal husbandry. In the first project, an in vitro model is used to study the influence of low antibiotic concentrations due to carry-over after production and usage of medicated feed on the development of resistance in the pig gut. Part of that project is to develop a quantitative risk assessment model. A second project focuses on tracking excreted antibiotics used in pig rearing and their influence on the development of antibiotic resistance in pig manure and the environment. In the last project, the relation between the use of biocides in animal husbandry and antibiotic resistance development are being studied. Copyright

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“…The mass spectrometric equipment consisted of a Xevo TQ-S (Waters) equipped with a Z-spray system. The conditions were described previously by Van den Meersche et al (2016).…”

Section: Liquid Chromatography-mass Spectrometrymentioning

confidence: 99%

Presence and fate of antibiotic residues, antibiotic resistance genes and zoonotic bacteria during biological swine manure treatment

Meersche

Rasschaert

Haesebrouck

et al. 2019

Ecotoxicology and Environmental Safety

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A B S T R A C TThe presence and dissemination of antibiotic residues, antibiotic resistance genes and zoonotic bacteria in the environment is of growing concern worldwide. Manure management practices, such as biological removal of nitrogen from swine manure, may help to decrease levels of antibiotic residues, antibiotic resistance genes and zoonotic bacteria present in manure before fertilization, thereby reducing environmental contamination. Therefore, the aim of this study was to monitor the presence and fate of seven antibiotic residues (colistin, sulfadiazine, trimethoprim, doxycycline, oxytetracycline, ceftiofur and tylosin A), nine antibiotic resistance genes (tet(B), tet(L), tet(M), tet(O), tet(Q), tet(W), erm(B), erm(F) and sul2) and two zoonotic bacteria (Salmonella Typhimurium and Campylobacter coli) during biological nitrogen removal from swine manure over time. Samples from the raw manure, the solid fraction, the liquid fraction and the storage lagoon were analyzed on two farms at six time points with an interval of two weeks. Only the antibiotics which were used during the three months preceding the first sampling could be detected before and after biological nitrogen removal from swine manure. Of all the antibiotics studied, doxycycline was recovered in all of the samples and sulfadiazine was recovered in most samples on both farms. For both antibiotics, there appears to be a reduction of the amount of residues present in the storage lagoon compared to the liquid fraction, however, this reduction was not statistically significant. A significant reduction of the relative abundances of most of the antibiotic resistance genes studied was observed when comparing the liquid fraction and the storage lagoon. For tet(L), no differences were observed between the fractions sampled and for sul2 and erm(F), a significant increase in relative abundances was observed on the second farm sampled. For the zoonotic bacteria, a reduction of at least 1 log was observed after biological nitrogen removal from swine manure. The results indicate that the concentration of certain antibiotic residues and several antibiotic resistance genes and the amount of zoonotic bacteria present in the manure may be reduced in the end product of the biological nitrogen removal from swine manure. Coördinatiecentrum Mestverwerking, 2017). For the biological removal https://doi.

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Development, validation and application of an ultra high performance liquid chromatographic-tandem mass spectrometric method for the simultaneous detection and quantification of five different classes of veterinary antibiotics in swine manure

Cited by 78 publications

References 27 publications

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Detection, Occurrence and Fate of Emerging Contaminants in Agricultural Environments

Antibiotic use and resistance in animals: Belgian initiatives

Presence and fate of antibiotic residues, antibiotic resistance genes and zoonotic bacteria during biological swine manure treatment

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