Development, validation and transfer of a hydrophilic interaction liquid chromatography/tandem mass spectrometric method for the analysis of the tobacco‐specific nitrosamine metabolite NNAL in human plasma at low picogram per milliliter concentrations

Pan, Jiongwei; Song, Qi; Shi, Huidong; King, Melissa E.; Junga, Heiko; Zhou, Shaolian; Weng, Naidong

doi:10.1002/rcm.1656

Cited by 43 publications

(29 citation statements)

References 19 publications

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“…Urinary nicotine and its metabolites were analysed using solid phase extraction and liquid chromatography with tandem mass spectrometry based on the method described by St. Charles and colleagues [26]. NNAL and its metabolites were analysed using a solid phase extraction and a LC/MS/MS method based on that of Xia and colleagues and Pan and colleagues [27,28].…”

Section: Urine Collection and Biomarker Analysesmentioning

confidence: 99%

Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins

Andreoli¹,

Bassi²,

Gregg³

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: Despite the well-documented role of cigarette smoke in the development of chronic obstructive pulmonary disease (COPD), lung cancer and cardiovascular disease, biomarkers for screening or monitoring disease progression and outcome remain elusive, particularly for COPD and lung cancer. Inflammatory cells and mediators are likely to be involved in the disease processes, but their importance is still poorly understood. The purpose of this study was to investigate early changes in immunological markers associated with smoking in healthy monozygotic twins without a detectable disease discordant for smoking, thereby minimising data variability due to genetic background. Methods: Twenty-two monozygotic twin pairs, aged 31.5 ± 6.3 years, entered the study. One of each twin pair was a smoker and the other a non-smoker. None of the subjects reported any diseases or clinically defined respiratory symptoms or airflow limitation. Each subject donated blood samples for determination of total leukocytes and subpopulations, lymphocyte subpopulation plus pro-inflammatory mediators (interleukin-8, tumour necrosis factor-α, soluble tumour necrosis factor-α receptors and C-reactive protein). Results: We observed a significant increase in the number of circulating leukocytes and neutrophils in smokers compared to non-smokers. Smokers also had significantly higher numbers of B cells and CD4 + T cells, plus an increased CD4/CD8 ratio. The numbers of NK cells were statistically significant lower in smokers compared to non-smokers.

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Section: Urine Collection and Biomarker Analysesmentioning

confidence: 99%

Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins

Andreoli¹,

Bassi²,

Gregg³

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Eight biomarkers of exposure were measured in 24h urine from these groups (nicotine, cotinine, hydroxycotinine, NNAL and all of the respective glucuronide conjugates). NNAL and its metabolite were analyzed using a solid phase extraction and liquid [11] and Pan et al [12] (LLOQ 5ng/ml ULOQ 1000ng/ml). Urinary nicotine and its metabolites were analyzed using a solid phase extraction and liquid chromatography with tandem mass spectrometry as described by St. Charles et al [6] (LLOQ 10ng/ml ULOQ 5000ng/ml).…”

Section: Materials and Methodologymentioning

confidence: 99%

The Relationship Between Cigarette Smoking and Biomarkers of Exposure Across Two Study Centers in Europe

Lowe¹,

Gregg²,

Bassi³

et al. 2010

TOBIOMJ

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Abstract:The relationship between biomarkers of exposure to cigarette smoke in 24h urine samples collected from groups of 80 smokers (44 males, 36 females) and 40 never smokers (17 males, 23 females) at two centers in Europe was studied. Eight biomarkers (nicotine, cotinine, hydroxycotinine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and all of the respective glucuronide conjugates) were measured. Subjects from the two centers were pooled and biomarker data analyzed according to the machine smoked tar yield of the brand each subject smoked and the recorded number of cigarettes smoked per day (CPD). A statistically significant relationship between CPD and all of the biomarkers analyzed was found. Smokers of less than 11 CPD had the lowest mean 24h urinary concentrations for all biomarkers measured. However, if the amount of constituent obtained from each cigarette smoked was calculated, then the amount of nicotine obtained per cigarette was highest in this group although the variation was also greatest for this group. The amount of NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1 butanone, the parent molecule of NNAL) obtained per cigarette was not statistically significantly different across all groups. In conclusion, these results confirm the reliability of 24h urinary total nicotine and NNAL concentrations as biomarkers of exposure to specific cigarette smoke constituents across two centers in Europe. These measurements may provide an objective alternative to CPD when grouping smokers for are studies of other endpoints.

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“…Use of standardized automation equipment has shown to be advantageous during the method transfer. Unfortunately very limited information on method transfer can be found in the literatures [35,149]. Typically in our organization, before the method transfer, scientists from both sites go through the method details very carefully.…”

Section: Methods Transfermentioning

confidence: 99%

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

Zhou¹,

Song²,

Tang³

et al. 2005

CPA

Self Cite

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Swift growth in the use of LC-MS/MS for the analysis of drugs in biological matrices has been compelled by the need for timely and high-quality data at many stages in drug discovery and development process: from high throughput screening of drug candidates and rapid data generation for pre-clinical studies to almost 'real-time' analysis of clinical samples. Prompt and rational method development, validation, and transfer play a pivotal role in achieving the goals of "faster, better, and cheaper" for pharmacokinetic studies since this could easily account for more than 50% of the time and labor resources for a moderate-sized project. Strategy for rational method development, validation and transfer has been largely kept as institutional knowledge but rarely appeared in literature. In this review article, strategies for developing and validating robust high throughput LC-MS/MS methods will be critically reviewed and discussed. Automated sample preparation, fast chromatography, minimization of matrix effects, and strategy of narrowing the gap between validation and incurred sample analysis are just a few topics covered in this review. Other interesting approaches for improving method efficiency and ruggedness such as direct injection SPE and liquid/liquid extracts as well as multiplexing of LC columns will also be discussed. Potential pitfalls during method development and validation are pointed out. At the end, the question "how fast is fast enough and how fast is too fast?" will be answered after considering all aspects of the method development and validation.

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Development, validation and transfer of a hydrophilic interaction liquid chromatography/tandem mass spectrometric method for the analysis of the tobacco‐specific nitrosamine metabolite NNAL in human plasma at low picogram per milliliter concentrations

Cited by 43 publications

References 19 publications

Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins

Effects of cigarette smoking on circulating leukocytes and plasma cytokines in monozygotic twins

The Relationship Between Cigarette Smoking and Biomarkers of Exposure Across Two Study Centers in Europe

Critical Review of Development, Validation, and Transfer for High Throughput Bioanalytical LC-MS/MS Methods

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