Developmental and transcriptomic responses of Hawaiian bobtail squid early stages to ocean warming and acidification

Abstract

Cephalopods play a central ecological role across all oceans and realms. However, under the current climate crisis, their physiology and behaviour are impacted, and we are beginning to comprehend the effects of environmental stressors at a molecular level. Here, we study the Hawaiian bobtail squid (Euprymna scolopes), known for its specific binary symbiosis with the bioluminescent bacteriumVibrio fischeriacquired post-hatching. We aim to understand the response (i.e., developmental and molecular) ofE. scolopesafter the embryogenetic exposure to different conditions: i) standard conditions (control), ii) increased CO2(∆pH 0.4 units), iii) warming (+3°C), or iv) a combination of the two treatments. We observed a decrease in hatching success across all treatments relative to the control. Using transcriptomics, we identified a potential trade-off in favour of metabolism and energy production, at the expense of development under increased CO2. In contrast, elevated temperature shortened the developmental time and, at a molecular level, showed signs of alternative splicing and the potential for RNA editing. The data also suggest that the initiation of the symbiosis may be negatively affected by these environmental drivers of change in the biosphere, although coping mechanisms by the animal may occur.