Developmental aspects of polyamine-oxidizing enzyme activities in the mouse kidney. Effects of testosterone

Abstract

In the present study developmental patterns of renal polyamine-oxidizing enzymes polyamine oxidase (PAO) and diamine oxidase (DAO) in male and female ICR mice were demonstrated. The effects of testosterone (10 micrograms/100g body weight) on renal PAO and DAO activities were also studied. The differences between sexes in both PAO and DAO activities were most clearly expressed in the immature kidney. At the age of 20 days PAO and DAO activities were 1.52 fold (p < 0.01) and 1.75 (p < 0.02) respectively higher in male mouse kidney than in female. Maturational processes reflected in significant increases in polyamine-oxidizing enzyme activities mainly in female mouse kidney, comparable with the gain in the kidney wet weight. Our data show that testosterone is able to influence renal PAO and DAO activities in addition to the well-known stimulation of polyamine biosynthesis. The hormonal effects were sex and age dependent. The influence of testosterone on renal PAO activity was mainly age dependent. The slight stimulation of renal PAO activity observed in 20- and 50-day old mice, 24 h after testosterone administration, change with a decrease in the enzyme activity at the age of 70 days. The effects of testosterone on renal DAO activity were mainly sex dependent. Testosterone caused stimulation of DAO activity with a very close magnitude (nearly twice) in female mouse kidney, independently of the age of mice. In contrast, in male mice the hormone treatment resulted in a statistically significant increase in renal DAO activity at the age of 70 days (1.3 fold, p < 0.05) only. It could be suggested that our data indicate the different contribution of renal PAO and DAO in androgen regulation of polyamine levels, depending on sex and the stage of the postnatal development.

Nitrogen application and different water regimes at booting stage improved yield and 2-acetyl-1-pyrroline (2AP) formation in fragrant rice

“…The diamine oxidase (DAO) activity was detected by using the methods as described by Jotova et al (1999), Su et al (2005), Xing et al (2007) and Yang et al (2011). The reaction solutions (3.0 ml) contained 2.5 mL 0.1 M sodium phosphate buffer (pH 6.5), 0.1 mL crude enzyme extracts, 0.1 mL peroxidase (250 U mL − 1 ) and 0.2 mL 4-aminoantipyrine / N, N-dimethylaniline.…”

Influence of testosterone on regulation of ODC, antizyme, andN1-SSAT gene expression in mouse kidney

“…One of these two scenarios must clearly occur, because similar high levels of Spd and Spm were found in male and female kidneys. The presence of N 1 -SSAT and PAO in male and female kidneys (14) indicates that catabolism of Spd and Spm takes place and leads to Put production. However, it seems that the interconversion pathway is not sufficient to compensate for the lack of ODC activity and to supply enough Put to the female mouse kidney.…”

“…Enzymes involved in polyamine metabolism, such as ODC, SAMDC, and PAO, are known to be especially abundant in the male mouse kidney (7,12,14,22). ODC mRNA, protein, and enzyme activity have been clearly localized by various techniques in the renal cortex, particularly in the proximal convoluted tubule (PCT) of the male mouse (7,18,21,31).…”

Characterisation of the pathogenic effects of the in vivo expression of an ALS-linked mutation in D-amino acid oxidase: Phenotype and loss of spinal cord motor neurons

“…Whilst body weight was lower in female double transgenic mice compared to SOD1 G93A mice, in males both SOD1 G93A and double transgenic animals showed a similar body mass ( Fig 4 ). This differential effect in females may reflect an endocrine effect, as testosterone is known to stimulate DAO activity in mouse kidney [ 28 ].…”