Developmental changes in glycolipids and synchronized expression of nutrient transporters in the mouse small intestine

Yoneshige, Azusa; Sasaki, Ayano; Miyazaki, Masao; Kojima, Naoya; Suzuki, Akemi; Matsuda, Junko

doi:10.1016/j.jnutbio.2008.12.009

Cited by 20 publications

(14 citation statements)

References 62 publications

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“…Henning (208) provides a good overview of GI development in mammals, especially in the laboratory rat, the most studied of about a dozen mammalian species that have been surveyed to date (3, 17, 49, 56, 57, 65, 134, 196, 219, 238, 263, 294, 323, 347, 362, 390, 394, 397, 433, 471, 483, 489, 490, 492). During the gestational phase, organs undergo morphological maturation [see also reference (354)] and many proteins required for digestion and absorption of components of milk are expressed (e.g., amino acid transporters and the glucose transporter SLGT1).…”

Section: Matches Of Gi System To Nutritional Changes During Ontogenymentioning

confidence: 99%

Comparative Digestive Physiology

Karasov

Douglas

2013

Comprehensive Physiology

261

221

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In vertebrates and invertebrates, morphological and functional features of gastrointestinal (GI) tracts generally reflect food chemistry, such as content of carbohydrates, proteins, fats, and material(s) refractory to rapid digestion (e.g., cellulose). The expression of digestive enzymes and nutrient transporters approximately matches the dietary load of their respective substrates, with relatively modest excess capacity. Mechanisms explaining differences in hydrolase activity between populations and species include gene copy number variations and single-nucleotide polymorphisms. Transcriptional and posttranscriptional adjustments mediate phenotypic changes in the expression of hydrolases and transporters in response to dietary signals. Many species respond to higher food intake by flexibly increasing digestive compartment size. Fermentative processes by symbiotic microorganisms are important for cellulose degradation but are relatively slow, so animals that rely on those processes typically possess special enlarged compartment(s) to maintain a microbiota and other GI structures that slow digesta flow. The taxon richness of the gut microbiota, usually identified by 16S rRNA gene sequencing, is typically an order of magnitude greater in vertebrates than invertebrates, and the interspecific variation in microbial composition is strongly influenced by diet. Many of the nutrient transporters are orthologous across different animal phyla, though functional details may vary (e.g., glucose and amino acid transport with K+ rather than Na+ as a counter ion). Paracellular absorption is important in many birds. Natural toxins are ubiquitous in foods and may influence key features such as digesta transit, enzymatic breakdown, microbial fermentation, and absorption

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Section: Matches Of Gi System To Nutritional Changes During Ontogenymentioning

confidence: 99%

Comparative Digestive Physiology

Karasov

Douglas

2013

Comprehensive Physiology

261

221

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“…In addition, species possessing odd number FAs such as C23:0, C23:0h and C25:0h were detected though these populations were small. These results are probably obtained because LS174T cells are derived from the intestine, not because they are cancer cell as mammalian intestinal epithelial cells abundantly express GSLs possessing t18:0-HFAs [1,2]. However, under hypoxia, we observed unexpected outcomes.…”

Section: Sphingomyelinsmentioning

confidence: 63%

“…Thus, the constituent ceramide species of the GSLs were distinctly different from those of the Cers and sphingomyelins in Caco2 cells, and they changed individually according to changes in oxygen levels. Usually intestinal cells abundantly express HFAs and/or phytosphingosine (t18:0) [1,2]. However, Caco2-cells do not express t18:0 in their constitutional ceramide of GSLs; therefore, we employed another human colon cancer cell line LS174T, which expresses large amounts of t18:0 and HFAs, to investigate the influence of hypoxia on the constituent ceramide composition of the GSLs with t18:0.…”

Section: Introductionmentioning

confidence: 99%

Hypoxia remodels the composition of the constituent ceramide species of HexCer and Hex2Cer with phytosphingosine and hydroxy fatty acids in human colon cancer LS174T cells

et al. 2015

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Oxygen-requiring enzymes, such as Δ4-desaturase (dihydroceramide desaturase), sphingolipid Δ4-desaturase/C-4-hydroxylase, and fatty acid 2-hydroxylase are involved in ceramide synthesis. We prepared free ceramides, sphingomyelins and glycosphingolipids (GSLs) from cancer cells cultivated under conditions of normoxia and hypoxia, and analyzed these compounds using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Human colon cancer LS174T cells were employed because these cells highly express hydroxyl fatty acids and phytosphingosine (t18:0) which are expected to be greatly influenced by changes in oxygen levels. As expected, the populations of dihydro-species of free ceramide and sphingomyelin with C16:0 non-hydroxy fatty acid were elevated, and the populations of HexCers and Hex2Cers, composed of C16:0 or C16:0 hydroxy fatty acid (C16:0h), and sphingosine (d18:1) or t18:0, were decreased under hypoxia. However, appreciable populations of HexCer and Hex2Cer species of C24:0 or C24:0h and t18:0 remained. These results suggest that the individual species of GSLs with fatty acids possessing different alkyl chain lengths, either non-hydroxy fatty acids or hydroxyl fatty acids, may be metabolized individually.

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“…We next examined the GSL composition of intestinal mucosa, where NPC1L1-mediated cholesterol absorption occurs. It has been reported that intestinal GM3S expression level is high in neonatal mice and declines during the course of development (29). We detected GM3 expression in WT and ApoE shl mice by TLC and LC/MS/MS analyses.…”

Section: Figmentioning

confidence: 83%