Developmental changes in proteins of casein micelles in goat milk using data-independent acquisition-based proteomics methods during the lactation cycle

Insights from 4D Label-Free Proteomic Analysis into Variation of Milk Fat Globule Membrane Proteins of Human Milk Associated with Infant’s Gender

“…6,7 In our earlier study, nearly 1596 MFGM proteins were identified using a data-independent acquisition (DIA) proteomic technique. 14 Variations in the protein numbers may be due to the differences in milk sources, protein preparation, and detection techniques used. Compared with previous studies, 6−8,13,14,19−22 some proteins related to immunity, digestion, and growth and development such as C8 β chain, tumor necrosis factor α-induced protein 8-like protein 2, epoxide hydrolase 1, and ephrin type-B receptor were first found in the human milk.…”

“…The gender of infants was carefully recorded in the questionnaire and participating volunteers were requested to sign a written consent. To reduce individual differences, the milk samples were mixed according to previous studies with some modifications . Milk samples were randomly pooled in biological triplicate in equal parts of every 4 individual samples.…”

“…To make the results more specific, Korean women in northeast China were selected since there are obvious distinctions between people of ethnic groups. 14 This study may provide useful information for a deep understanding of the nutritional composition of human milk. Additionally, it may offer a new idea to design and develop infant formula food for boys or girls based on the composition variation of human milk.…”

Triglyceride and fatty acid composition of bovine colostrum and transition milk in pasture‐based dairy cows supplemented prepartum with inorganic selenium, organic selenium or rumen‐protected choline

“…Contarini et al (2014) also demonstrated that time post-partum did not have a significant effect on the levels of CN24. Colostrum TAGs play an important role in lipid absorption and the transportation of low-density lipoproteins (Sun et al 2023). Limited information is available on the TAG composition of bovine colostrum.…”

Identification markers of goat milk adulterated with bovine milk based on proteomics and metabolomics

“…In autumn of 2021, approximately 10 L of goat and Holstein tank milk samples (4 °C) were obtained on three different days from the farms of Qingdao, China. Regarding changes in milk proteins along the lactation stages ( Sun et al, 2023 ), tank mature milk was collected and applied in this study. Milk samples were placed on ice bags and transferred to the laboratory within 1–2 h and stored at −80 °C; 50 mL milk for each sample was analyzed using MilkoScan FT 120 (FOSS, Hillerød, Denmark) for milk protein, fat, and lactose.…”