Developmental changes of the oestrogen receptor-alpha and -beta mRNAs in the female reproductive organ of the rat--an analysis by in situ hybridization

Mowa, Chishimba Nathan; Iwanaga, Toshihiko

doi:10.1677/joe.0.1670363

Cited by 47 publications

(41 citation statements)

References 42 publications

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“…Species-specific differences could also explain the expression of ERα in the Mullerian duct of fetal mice (Nielsen et al, 2000) and rats (Mowa and Iwanaga, 2000), while in the fetal porcine Mullerian duct only ERβ was detected.…”

Section: Discussionmentioning

confidence: 99%

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Knapczyk

Duda

Szafrańska

et al. 2008

Acta Veterinaria Hungarica

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The sites of oestrogen action can be shown by the localisation of their receptors in the target tissues. The aim of the present study was to show the localisation of oestrogen receptors in porcine embryos and fetuses obtained on days 18, 22, 32, 40, 50, 60, 71 and 90 post coitum (p.c.). The visualisation of proteins was conducted in embryos and various fetal organs such as gonads, uterus, lung, kidney, intestine and adrenal gland. Both ERs were observed in the blastocysts on day 18 p.c. In the male, ERβ was detected in the testis and epididymis, whereas ERα was present in the efferent ductules. In the female, ERβ was detected in the ovarian stromal cells investing the oocyte nests, while ERα protein was detected in the surface epithelium. In the uterus, ERs were present in the stromal cells, while ERβ was present in the luminal epithelium. In the non-reproductive fetal porcine tissues ERβ was localised in the lungs, kidneys, adrenal glands and in the umbilical cords. Both ERs were observed in the intestine. It is possible that ERβ may play important roles in the development of the adrenal gland, testis, kidney and lungs, while both ERs are involved in the development of the ovary, uterus, epididymis and intestine of the porcine fetus.

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Section: Discussionmentioning

confidence: 99%

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Knapczyk

Duda

Szafrańska

et al. 2008

Acta Veterinaria Hungarica

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“…Two in situ hybridization studies revealed similar negative results in the epithelium and mesenchyme of the Müllerian duct throughout the prenatal period in rats and mice (Lemmen et al, 1999;Mowa and Iwanaga, 2000b). However, in the RT-PCR study, rat Müllerian duct ERβ1 and ERβ2 mRNAs were detected and found to be expressed at constant levels from 15.5 dpc to 21.5 dpc (Okada et al, 2002a).…”

Section: During Prenatal Development Of the Müllerian Ductmentioning

confidence: 81%

“…Greco and co-workers (1991) reported that ER immunoreactivity was observed in epithelial cells of female mouse reproductive tract at 15 days post-coitus (dpc), but was occasionally observed at 17 dpc and on the day of birth. The results of immunohistochemistry with a monoclonal antibody specific to ERα in mice (Nielsen et al, 2000), and in situ hybridization with a radio-labeled probe specific to ERα in rats (Mowa and Iwanaga, 2000b) demonstrate that ERα localization varies with the region of the Müllerian duct involved. Nielsen et al (2000) detected a faint immunopositive signal in the nuclei of the surrounding cells of the Müllerian duct as early as 11.5 dpc in mice.…”

Section: During Prenatal Development Of the Müllerian Ductmentioning

confidence: 97%

“…ERβ is reportedly expressed at a lower level than ERα in the uterus and vagina of adult mice and rats (Mowa and Iwanaga, 2000a;Shughrue et al, 1998;Wang et al, 1999). ER is expressed in the Müllerian duct of fetal mice (Greco et al, 1991;Wu et al, 1992;Greco et al, 1993;Lemmen et al, 1999;Jefferson et al, 2000;Nielsen et al, 2000) and rats (Mowa and Iwanaga, 2000b). Greco and co-workers (1991) reported that ER immunoreactivity was observed in epithelial cells of female mouse reproductive tract at 15 days post-coitus (dpc), but was occasionally observed at 17 dpc and on the day of birth.…”

Section: During Prenatal Development Of the Müllerian Ductmentioning

confidence: 99%

See 1 more Smart Citation

Sex Steroid Hormone Receptors in the Developing Female Reproductive Tract of Laboratory Rodents

Okada¹,

Satō

Ohta

et al. 2005

J. Toxicol. Sci.

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-Many chemicals released into the environment potentially disrupt the endocrine system in wildlife and humans. Some of these chemicals exhibit estrogenic activity by binding to the estrogen receptors. The developing organism is particularly sensitive to estrogenic chemicals during the critical period in which the induction of long-term changes and persistent molecular alterations in female reproductive tracts occur. Perinatal mouse and rat models can be utilized as indicators for determining the consequences of exposure to exogenous estrogenic agents, including possible xenoestrogens or environmental endocrine disruptors. Estrogen receptors (ER) and estrogen responsive genes, therefore, need to be identified in order to understand the molecular basis of estrogenic actions. Recent identifications of ER subtypes and isoforms make understanding target organ responses to these estrogenic chemicals even more difficult. Indeed, many reports suggest that these chemicals do affect the reproductive and developmental processes of female laboratory rodents that had been perinatally exposed, and that interactions between sex steroid hormone receptors occur. Much information concerning the expression of sex steroid receptors in rodents has been reported concerning the normal development of the Müllerian duct. Thus, accumulated information on the expression of ER subtypes and isoforms as well as that of progesterone and androgen receptors in laboratory rodents is herein reviewed, in addition to the presentation of our own data.

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“…Excluding some splicing variants, two major estrogen receptor (ER) forms, namely ERα and ERβ are expressed in both the epithelial and stromal cells of the rodent uterus [21][22][23][24][25][26][27]. Although ERβ is detected to a lower level and exclusively in the late phase of gestation, both forms are expressed throughout gestational development in a temporalspecific manner [5].…”

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Expression Pattern of Gadd45g Signaling Pathway Components in the Mouse Uterus Following Hormonal Steroid Treatments

2012

Endocrinol Metab Synd

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The uterus is a hormone-dependent organ under the control of estrogens, progestins and androgens. The actions of estradiol (E2) and progesterone on uterine physiology are well documented, while active androgens such as testosterone and dihydrotestosterone (DHT) are now also recognized to exert an important role to appropriately maintain the cyclical changes of the endometrium.Based on our previous observations that DHT modulates GADD45g and the expression of specific cell cycle genes, the aim of this study was to identify the specific uterine cell types in which these cell cycle genes are modulated by hormonal steroids.Using in situ hybridization and quantitative RT-PCR (QRT-PCR), the localization and measurement of mRNA expression of key cell cycle genes responding to E2 and DHT were performed in the uterus of ovariectomized mice. Interestingly, in situ hybridization experiments demonstrate that Gadd45g mRNA is detected early and strongly in stromal cells only, indicating that the early cell cycle arrest induced by DHT and E2 in the mouse uterus occurs in this compartment. On the other hand, the cell cycle stimulation represented by the late increase of Ccnb1, Cdc2a and Cdc25c gene expression is located exclusively in the glandular and luminal epithelial cells. QRT-PCR experiments confirm the regulation of mRNA expression observed following in situ hybridization. Surprisingly, both hormones appear to trigger effects in the same direction on cell cycle progression, with androgens inducing a lower modulation of gene expression levels. Additional experiments using the human uterine Ishikawa cell line confirmed the implication of the estrogen receptor in the GADD45g up-regulation following treatment with E2 while the regulation triggered by DHT is less clear. These observations illustrate clearly the stroma-epithelium interactions, which finely regulate the uterine physiology via paracrine mechanisms. Additional investigations are definitely needed to determine the exact role played by androgens in mouse uterine growth and differentiation.

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Developmental changes of the oestrogen receptor-alpha and -beta mRNAs in the female reproductive organ of the rat--an analysis by in situ hybridization

Cited by 47 publications

References 42 publications

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Immunolocalisation of oestrogen receptors alpha (ERα) and beta (ERβ) in porcine embryos and fetuses at different stages of gestation

Sex Steroid Hormone Receptors in the Developing Female Reproductive Tract of Laboratory Rodents

Spatiotemporal Expression Pattern of Gadd45g Signaling Pathway Components in the Mouse Uterus Following Hormonal Steroid Treatments

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