Developmental competence and mRNA expression of preimplantation in vitro–produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1

Zaraza, J.; Oropeza, A.; Velazquez, Miguel A.; Korsawe, Karin; Herrmann, Doris; Carnwath, Joseph Wallace; Niemann, Heiner

doi:10.1016/j.theriogenology.2009.11.033

Cited by 35 publications

(41 citation statements)

References 64 publications

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“…Epigenetic mechanisms, specifically DNA methylation dynamics, have been implicated in the low developmental capacity of juvenile oocytes (Oropeza et al 2004, Zaraza et al 2010. In the study presented here, we investigated for the first time the influence of donor age and hormonal treatments on the general DNA Methylation pattern of 'BTS sequence' in immature and in vitro matured oocytes of different experimental groups.…”

Section: Discussionmentioning

confidence: 99%

“…Only healthy animals with adequate development of the reproductive tract were included in the experiments. Oocytes were collected over 3-week periods at intervals of 3-4 days by ultrasound-guided OPU after the initial removal of the dominant follicle 4 days prior to the first OPU session (Zaraza et al 2010).…”

Section: Animals and Treatmentsmentioning

confidence: 99%

“…Ultrasound-guided OPU was performed as previously described (Oropeza et al 2004, Zaraza et al 2010. Briefly, ovaries were visualized using a CS9000 ultrasound system (Picker, Mü nchen, Germany) and a 6.5 MHz ultrasound transducer (Picker model, EUP-F-331).…”

Section: Ultrasound Equipment and Oocyte Retrievalmentioning

confidence: 99%

“…However, currently the average rates of viable blastocysts produced from prepubertal donor oocytes are lower than the use of adult oocytes (10-15 vs w30%; Oropeza et al 2004, Zaraza et al 2010. Incomplete or deficient cytoplasmic maturation, altered protein synthesis, reduced oocyte size, and impaired metabolism in prepubertal oocytes have been proposed as possible mechanisms for the reduced developmental potential (Lonergan et al 1994, Steeves et al 1999, De Paz et al 2001, Kauffold et al 2005.…”

Section: Introductionmentioning

confidence: 99%

“…Embryos produced from oocytes derived from IGF1-treated prepubertal donors showed similar developmental potential and a similar mRNA expression profile of genes critical in embryonic development as those produced from adult donors (Oropeza et al 2004). Epigenetic factors were proposed to be involved in the acquisition of full developmental competence of bovine prepubertal oocytes, but have not yet been studied in detail (Oropeza et al 2004, Zaraza et al 2010.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Diederich¹,

Hansmann²,

Heinzmann³

et al. 2012

Reproduction

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The developmental capacity of oocytes from prepubertal cattle is reduced compared with their adult counterparts, and epigenetic mechanisms are thought to be involved herein. Here, we analyzed DNA methylation in three developmentally important, nonimprinted genes (SLC2A1, PRDX1, ZAR1) and two satellite sequences, i.e. 'bovine testis satellite I' (BTS) and 'Bos taurus alpha satellite I' (BTaS). In parallel, mRNA expression of the genes was determined by quantitative real-time PCR. Oocytes were retrieved from prepubertal calves and adult cows twice per week over a 3-week period by ultrasound-guided follicular aspiration after treatment with FSH and/or IGF1. Both immature and in vitro matured prepubertal and adult oocytes showed a distinct hypomethylation profile of the three genes without differences between the two types of donors. The methylation status of the BTS sequence changed according to the age and treatment while the methylation status of BTaS sequence remained largely unchanged across the different age and treatment groups. Relative transcript abundance of the selected genes was significantly different in immature and in vitro matured oocytes; only minor changes related to origin and treatment were observed. In conclusion, methylation levels of the investigated satellite sequences were high (O50%) in all groups and showed significant variation depending on the age, treatment, or in vitro maturation. To what extent this is involved in the acquisition of developmental competence of bovine oocytes needs further study.

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Section: Discussionmentioning

confidence: 99%

Section: Animals and Treatmentsmentioning

confidence: 99%

Section: Ultrasound Equipment and Oocyte Retrievalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

Diederich¹,

Hansmann²,

Heinzmann³

et al. 2012

Reproduction

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DNA methylation and mRNA expression of developmentally important genes in bovine oocytes collected from donors of different age categories

Mattern¹,

Herrmann²,

Heinzmann³

et al. 2016

Mol. Reprod. Dev.

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Epigenetic changes are critical for the acquisition of developmental potential by oocytes and embryos, yet these changes may be sensitive to maternal ageing. Here, we investigated the impact of maternal ageing on DNA methylation and mRNA expression in a panel of eight genes that are critically involved in oocyte and embryo development. Bovine oocytes were collected from donors of three different age categories-prepubertal (9-12 months old), mature (3-7 years old), and aged (8-11 years old)-and were analyzed for gene-specific DNA methylation (bTERF2, bREC8, bBCL-XL, bPISD, bBUB1, bDNMT3Lo, bH19, and bSNRPN) and mRNA expression (bTERF2, bBCL-XL, bPISD, and bBUB1). A total of 1,044 alleles with 88,740 CpGs were amplified and sequenced from 362 bovine oocytes. Most of the detected molecules were either fully methylated or completely unmethylated. Only 9 out of 1,044 alleles (<1%) were abnormally methylated (>50% of CpGs with an aberrant methylation status), and seven of the nine abnormally methylated alleles were within only two candidate genes (bDNMT3Lo and bH19). No significant differences were detected with regard to mRNA expression between oocytes from the three groups of donors. These results suggest that genes predominantly important for early embryo development (bH19 and bDNMT3Lo) are less resistant to abnormal methylation than genes critically involved in oocyte development (bTERF2, bBCL-XL, bPISD, bBUB1, and bSNRPN). Establishment of DNA methylation in bovine oocytes seems to be largely resistant to changes caused by maternal ageing, irrespective of whether the genes are critical to achieve developmental competence in oocytes or early embryos. Mol. Reprod. Dev. 83: 802-814, 2016 © 2016 Wiley Periodicals, Inc.

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DNA methylation status of bovine blastocysts obtained from peripubertal oocyte donors

Morin-Doré

Blondin²,

Vigneault³

et al. 2020

Molecular Reproduction Devel

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In the dairy industry, the high selection pressure combined with the increased efficiency of assisted reproduction technologies (ART) are leading toward the use of younger females for reproduction purposes, with the aim to reduce the interval between generations. This situation could impair embryo quality, decreasing the success rate of the ART procedures and the values of resulting offspring. Young Holstein heifers (n = 10) were subjected to ovarian stimulation and oocyte collection at 8, 11, and 14 months of age. All the oocytes were fertilized in vitro with semen from one adult bull, generating three pools of embryos per animal. Each animal was its own control for the evaluation of the effects of age. The EmbryoGENE platform was used to compare the DNA methylation status of blastocysts obtained from oocytes collected at 8 versus 14 and 11 versus 14 months of age. Age‐related contrast analysis identified 5,787 and 3,658 differentially methylated regions (DMRs) in blastocysts from heifers at 8 versus 14 and 11 versus 14 months of age, respectively. For both contrasts, the DMRs were distributed nonrandomly in the different DNA regions. The DNA from embryos from 8‐month‐old donors was more hypermethylated, while the DNA from embryos from 11‐month‐old donors displayed an intermediate phenotype. According to Ingenuity Pathway Analysis, the upstream regulator genes cellular tumor antigen p53, transforming growth factor β1, tumor necrosis factor, and hepatocyte nuclear factor 4α are particularly associated with methylation sensitive targets, which were more hypermethylated in embryos from younger donors.

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Developmental competence and mRNA expression of preimplantation in vitro–produced embryos from prepubertal and postpubertal cattle and their relationship with apoptosis after intraovarian administration of IGF-1

Cited by 35 publications

References 64 publications

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors

DNA methylation and mRNA expression of developmentally important genes in bovine oocytes collected from donors of different age categories

DNA methylation status of bovine blastocysts obtained from peripubertal oocyte donors

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