Developmental expression of lineage specific genes in porcine embryos of different origins

Kumar, B. Mohana; Maeng, Geun-Ho; Jeon, Ryoung-Hoon; Lee, Yeon Mi; Lee, Won Jae; Jeon, Byeong‐Gyun; Ock, Sun‐A; Rho, Gyu Jin

doi:10.1007/s10815-012-9797-8

Cited by 8 publications

(13 citation statements)

References 44 publications

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“…This finding was consistent with previous report indicating the low expression of SOX2 in porcine IVF, parthenogenetic, and NT embryos reconstructed with MSCs or fetal fibroblast (Kumar et al, 2012). However, the higher expression of this gene in post-hatching stages (Kumar et al, 2012) may indicate it's more important role during or after implantation.…”

Section: Igf2 Igf2rsupporting

confidence: 93%

“…In the present study, despite no significant difference in SOX2 expression between IVF and AF and MSC-NT embryos, the expression in AMSCs-NT derived blastocyst was higher than IVF embryos. It seems the expression of this gene is not associated with survival rate (Kumar et al, 2012) so that the lower SOX2 expression in IVF group has no adverse effect on embryo development in this group. This finding was consistent with previous report indicating the low expression of SOX2 in porcine IVF, parthenogenetic, and NT embryos reconstructed with MSCs or fetal fibroblast (Kumar et al, 2012).…”

Section: Igf2 Igf2rmentioning

confidence: 89%

“…It seems the expression of this gene is not associated with survival rate (Kumar et al, 2012) so that the lower SOX2 expression in IVF group has no adverse effect on embryo development in this group. This finding was consistent with previous report indicating the low expression of SOX2 in porcine IVF, parthenogenetic, and NT embryos reconstructed with MSCs or fetal fibroblast (Kumar et al, 2012). However, the higher expression of this gene in post-hatching stages (Kumar et al, 2012) may indicate it's more important role during or after implantation.…”

Section: Igf2 Igf2rmentioning

confidence: 89%

“…Regarding the use of stem cells as donor cells, the porcine MSC-NT reconstructed oocytes had a higher developmental rate compared to the fetal fibroblast-NT derived counterparts , Samiec et al, 2015. The higher appropriate remodeling of stem cell-NT derived embryos has proved in different species (Bosch et al, 2006, Faast et al, 2006, Kumar et al, 2012, Lee et al, 2006, Rideout et al, 2000. Some reports, however, have rejected transcendence of stem cells over somatic cells in normal reprogramming of NT derived embryos (Berg et al, 2007, Li et al, 2013, Sung et al, 2006.…”

Section: Discussionmentioning

confidence: 99%

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The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Nazari¹,

Shirazi²,

Shams‐Esfandabadi³

et al. 2016

Int. J. Dev. Biol.

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Nuclear reprogramming of a differentiated cell in somatic cell nuclear transfer (SCNT) is a major concern in cloning procedures. Indeed, the nucleus of the donor cell often fails to express the genes which are a prerequisite for normal early embryo development. This study was aimed to evaluate the developmental competence and the expression pattern of some reprogramming related genes in bovine cloned embryos reconstructed with amniotic membrane stem cells (AMSCs) in comparison with those reconstructed with mesenchymal stem cells (MSCs) and adult fibroblasts (AF) as well as with in vitro fertilized (IVF) oocytes. In vitro matured abattoir-derived oocytes were considered as recipients and a hand-made cloning technique was employed for oocyte enucleation and nuclear transfer (NT) procedures. The expression pattern of genes involved in self-renewal and pluripotency (POU5F1, SOX2, NANOG), imprinting (IGF2, IGF2R), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), and apoptosis (BAX, BCL2) were evaluated in NT and IVF derived embryos. Despite the insignificant difference in cleavage rate between reconstructed and IVF oocytes, the blastocyst rate in the IVF group was higher than that of other groups. Among reconstructed oocytes, a higher blastocysts rate was observed in MSC-NT and AMSCs-NT derived embryos that were significantly higher than AF-NT derived ones. There were more similarities in the expression pattern of pluripotency and epigenetic modification genes between MSC-NT and IVF derived blastocysts compared with other groups. In conclusion, considering developmental competence, AMSCs, as alternative donors in SCNT procedure, like MSCs, were prone to have more advantage compared with AF.

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Section: Igf2 Igf2rsupporting

confidence: 93%

Section: Igf2 Igf2rmentioning

confidence: 89%

Section: Igf2 Igf2rmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Nazari¹,

Shirazi²,

Shams‐Esfandabadi³

et al. 2016

Int. J. Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Differences between embryos produced by different in vivo and in vitro methods have revealed their distinctive characteristics including developmental efficiency, total cell number and gene expression (Kumar et al 2007(Kumar et al , 2012Mamo et al 2007;Magnani and Cabot 2008;McElroy et al 2008;du Puy et al 2011). In particular, compared with others, SCNT embryos have revealed extensively different characteristics caused by the process of manipulation attributable to incomplete and abnormal epigenetic reprogramming (Somers et al 2006;Beyhan et al 2007;Suzuki et al 2008;Zhao et al 2010;Deshmukh et al 2011;Kumar et al 2013).…”

mentioning

confidence: 99%

Selection of reference genes for quantitative real-time polymerase chain reaction in porcine embryos

Lee

Jang

Lee

et al. 2017

Reprod. Fertil. Dev.

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Abstract. To study gene expression and to determine distinctive characteristics of embryos produced by different methods, normalisation of the gene(s) of interest against reference gene(s) has commonly been employed. Therefore, the present study aimed to assess which reference genes tend to express more stably in single porcine blastocysts produced in vivo (IVO) or by parthenogenetic activation (PA), in vitro fertilisation (IVF) and somatic cell nuclear transfer (SCNT) using different analysis programs, namely geNorm, Normfinder and Bestkeeper. Commonly used reference genes including 18S rRNA (18S), H2A histone family, member Z (H2A), hypoxanthine phosphoribosyltransferase1 (HPRT1), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ribosomal protein 4 (RPL4), peptidylprolyl isomerase A (PPIA), beta actin (ACTB), succinate dehydrogenase complex, subunit A (SDHA) and hydroxymethylbilane synthase (HMBS2) were analysed; most of them resulted in significantly (P , 0.05) different cycle threshold (CT) values in porcine embryos except for SDHA and H2A. In evaluation of stable reference genes across in vivo and in vitro porcine blastocysts, three kinds of programs showed slightly different results; however, there were similar patterns about the rankings of more or less stability overall. In conclusion, SDHA and H2A were determined as the most appropriate reference genes for reliable normalisation in order to find the comparative gene expression in porcine blastocysts produced by different methods, whereas 18S was regarded as a less-stable reference gene. The present study has evaluated the stability of commonly used reference genes for accurate normalisation in porcine embryos to obtain reliable results.

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GDF8 enhances SOX2 expression and blastocyst total cell number in porcine IVF embryo development

et al. 2019

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Developmental expression of lineage specific genes in porcine embryos of different origins

Abstract: MSCs seem to be better donors for porcine NT as they improved the developmental competency, and influenced the expression pattern of genes quite similar with IVF embryos than that of FFs.

Cited by 8 publications

References 44 publications

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Selection of reference genes for quantitative real-time polymerase chain reaction in porcine embryos

GDF8 enhances SOX2 expression and blastocyst total cell number in porcine IVF embryo development

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