Developmental expression of VGF mRNA in the prenatal and postnatal rat

Snyder, Susan E.; Pintar, John E.; Salton, Stephen R.J.

doi:10.1002/(sici)1096-9861(19980427)394:1<64::aid-cne6>3.0.co;2-f

Cited by 46 publications

(32 citation statements)

References 20 publications

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“…Specific hybridization was first observed at midgestation in cranial, sympathetic and sensory ganglia (Snyder et al 1998), after which the distribution broadened throughout the nervous system. Expression in non-neural tissues was first noted at E11·5, not in the embryo proper but rather in the gravid uterus where strong VGF mRNA hybridization in a subpopulation of cells in the uterine wall was noted.…”

Section: Resultsmentioning

confidence: 99%

“…In situ hybridization was carried out essentially as previously described by Snyder et al (1998). Briefly, slides were fixed in RNase-free 4% paraformaldehyde, 50 mM NaCl, 100 mM sodium phosphate (pH 7·2) fixative for 2-15 min, rinsed and dehydrated through a graded ethanol series, then rehydrated and incubated in a 0·05 M triethanolamine, 0·026 M acetic acid solution for 2 min.…”

Section: Preparation Of Crna Probes and In Situ Hybridizationmentioning

confidence: 99%

“…Slides were then acetylated for 10 min, rinsed, and dehydrated. Sections were prehybridized for 1-2 h, hybridized overnight at 50 C, washed for 30 min twice, treated with ribonuclease A, then washed several times to a final stringency of 0·2 SSC at 65-70 C for 2 h, as previously detailed (Snyder et al 1998). In each experiment, the appropriate VGF sense-strand cRNA probe was hybridized to adjacent sections in parallel with antisense probe.…”

Section: Preparation Of Crna Probes and In Situ Hybridizationmentioning

confidence: 99%

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Expression of VGF mRNA in developing neuroendocrine and endocrine tissues

Snyder¹,

Peng²,

Pintar³

et al. 2003

Journal of Endocrinology

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Analysis of knockout mice suggests that the neurotropininducible secreted polypeptide VGF (non-acronymic) plays an important role in the regulation of energy balance. VGF is synthesized by neurons in the central and peripheral nervous systems (CNS, PNS), as well as in the adult pituitary, adrenal medulla, endocrine cells of the stomach and pancreatic beta cells. Thus VGF, like cholecystokinin, leptin, ghrelin and other peptide hormones that have been shown to regulate feeding and energy expenditure, is synthesized in both the gut and the brain. Although detailed developmental studies of VGF localization in the CNS and PNS have been completed, little is known about the ontogeny of VGF expression in endocrine and neuroendocrine tissues. Here, we report that VGF mRNA is detectable as early as embryonic day 15·5 in the developing rat gastrointestinal and esophageal lumen, pancreas, adrenal, and pituitary, and we further demonstrate that VGF mRNA is synthesized in the gravid rat uterus, together supporting possible functional roles for this polypeptide outside the nervous system and in the enteric plexus.

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Section: Resultsmentioning

confidence: 99%

Section: Preparation Of Crna Probes and In Situ Hybridizationmentioning

confidence: 99%

Section: Preparation Of Crna Probes and In Situ Hybridizationmentioning

confidence: 99%

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Expression of VGF mRNA in developing neuroendocrine and endocrine tissues

Snyder¹,

Peng²,

Pintar³

et al. 2003

Journal of Endocrinology

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“…Although one group was identifying downstream targets of exercise, and the other was focused on gene expression changes that were common following elevation of two putative mediators of antidepressant efficacy, the identification of VGF by both suggests a point of convergence between exercise and antidepressant signaling. Note that VGF is widely expressed in neurons in the brain and is involved in maintaining organismal energy balance, as well as in mediating hippocampal synaptic activity (19)(20)(21).…”

Section: Discovery Of Vgf As a Mediator Of Antidepressant Responsesmentioning

confidence: 99%

VGF, a New Player in Antidepressant Action?

Malberg

Monteggia

2008

Sci. Signal.

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Recent studies have identified adaptations of intracellular signaling pathways and target genes that could contribute or modulate the action of antidepressant drugs, as well as exercise-mediated antidepressant responses. Understanding these adaptations, particularly those changes that are common to diverse antidepressant treatments, is important for the development of more potent and specific treatments of depression. There is growing evidence that growth factors may be important mediators of antidepressant responses. Now, VGF (not an acronym), a neuropeptide that has previously been shown to be involved in maintaining organismal energy balance, as well as in mediating hippocampal synaptic plasticity, may be involved in mediating antidepressant responses. These studies use in vivo approaches to link VGF to the antidepressant-like behavioral effects produced by antidepressant drugs and exercise.

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“…VGF is a NGF-specific response gene. It has been reported that VGF mRNA was rapidly and markedly induced by neurotrophins such as NGF, peaking during axonal outgrowth and synaptogenesis [14]. Rab1 is a vesicular trafficking protein.…”

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confidence: 99%

Degradable PRGD/PDLLA/β-TCP/NGF composites promote differentiation and regulate gene expression in rat pheochromocytoma cells

Qiu

Yan

et al. 2013

Chin. Sci. Bull.

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In this study, we synthesized degradable PRGD/PDLLA/-TCP/NGF composites to facilitate neuronal repair. To this end, we (1) examined the release of nerve growth factor (NGF) from the composites, (2) evaluated the differentiation status of the cells and (3) address how transcriptional activity may regulate the differentiation mechanism of these cells. NGF content was determined using enzyme-linked immunosorbent assay, while the cellular mRNA expression was examined by real-time PCR analysis. Our results indicated that NGF release was robust during the first 10 days and then stabilized at a lower level thereafter. Treatment of PC12 cells with the extract of the NGF-embedded composites induced the formation of neurites and, in some cases, net-like neurites. Analysis of the expression level of differentiation-related genes, such as TrkA, VGF, Rab1, GAP43 and β-tubulin II, were significantly up-regulated. These findings suggest that these composites might be a suitable delivery system for growth factors like NGF that can be used to facilitate neuronal repair after injury.nerve guidance conduit, nerve growth factor, neuronal repair, PC12 cells Citation:Qiu T, Yu C, Yan Q J, et al. Degradable PRGD/PDLLA/-TCP/NGF composites promote differentiation and regulate gene expression in rat pheochromocytoma cells.

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Developmental expression of VGF mRNA in the prenatal and postnatal rat

Cited by 46 publications

References 20 publications

Expression of VGF mRNA in developing neuroendocrine and endocrine tissues

Expression of VGF mRNA in developing neuroendocrine and endocrine tissues

VGF, a New Player in Antidepressant Action?

Degradable PRGD/PDLLA/β-TCP/NGF composites promote differentiation and regulate gene expression in rat pheochromocytoma cells

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