Developmental potential of embryos from intracytoplasmic sperm injection cycles containing fragmented oocytes

Kaser, Daniel J.; Reichman, Daniël; Ginsburg, Elizabeth S.; Politch, Joseph A.; Racowsky, Catherine

doi:10.1016/j.fertnstert.2011.11.013

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…Intra-cytoplasmic abnormalities include dark cytoplasm, ooplasm granulation, irregular shape, refractile bodies (RF), bull eye (central aggregation of organelles and vesicles within ooplasm), smooth endoplasmic reticulum (SER) and vacuoles (Fancsovits et al , 2012). Oocyte morphology and its influence on embryo competence is still a controversial subject in the literature (Loutradis et al , 1999; Esfandiari et al , 2006; Nichi et al , 2011; Rienzi et al , 2011; Kaser et al , 2012; Braga et al , 2013; Sauerbrun-Cutler et al , 2015). Hence, the assessment of oocyte morphology remains one of the most significant purposes of ART advancement (Uyar et al ., 2013).…”

Section: Introductionmentioning

confidence: 99%

Oocyte morphology and embryo morphokinetics in an intra-cytoplasmic sperm injection programme. Is there a relationship?

Faramarzi

Khalili

Ashourzadeh

2017

Zygote

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The aim was to investigate the relationship between the morphological parameters of metaphase II (MII) oocytes with morphokinetic variables of embryos following an intra-cytoplasmic sperm injection (ICSI) procedure. Morphokinetic behaviour and abnormal cleavage patterns of 334 zygotes were analyzed using time-lapse monitoring (TLM). In addition, oocyte morphology was assessed in relation to embryo morphokinetic (absolute time point, including time to second polar body (PB) extrusion (ESPB), pronuclei (PN) appearance (PNA), PN fading (PNF), time to 2-cells (t2), 3c (t3), 4c (t4), 5c (t5), 6c (t6), 7c (t7), 8c (t8) and relative timing parameters (S1, S2, CC2 and CC3). Also, cleavage patterns (uneven blastomeres, reverse, direct and arbitrary) were assessed. The data showed that 79% of the normal fertilized oocytes had at least one abnormal morphological characteristic. Intra-cytoplasmic abnormalities were observed in 12% of the oocytes. Also, extra-cytoplasmic abnormalities were noticed in 29%, while combined intra- and extra-cytoplasmic abnormalities were responsible for the remaining 38% of the oocytes. Nearly all cleavage and interval times, except extrusion of the ESPB time (P = 0.003), were similar between normal and abnormal morphologic oocytes (P < 0.05). Moreover, there was significant relationship for oocyte morphology abnormalities and cleavage patterns, including uneven blastomere (P = 0.037), reverse cleavage (RC) (P = 0.0), direct (P = 0.001) and arbitrary cleavages (P = 0.001). Using TLM, the cleavage patterns of embryos were affected by the quality of MII oocytes in the ICSI cycles. So, evaluation of oocyte morphology with subsequent embryo morphokinetics is recommended in assisted reproductive programmes.

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Section: Introductionmentioning

confidence: 99%

Oocyte morphology and embryo morphokinetics in an intra-cytoplasmic sperm injection programme. Is there a relationship?

Faramarzi

Khalili

Ashourzadeh

2017

Zygote

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“…The relationship between oocyte quality, embryo development, and in vitro fertilization (IVF) outcomes has been widely studied [ 1 – 10 ]. Most IVF programs, ours included, rely almost exclusively on embryo morphology in selecting embryos for transfer.…”

Section: Introductionmentioning

confidence: 99%

Oocyte Scoring Enhances Embryo-Scoring in Predicting Pregnancy Chances with IVF Where It Counts Most

et al. 2015

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ContextOur center’s quality improvement optimization process on many occasions anecdotally suggested that oocyte assessments might enhance embryo assessment in predicting pregnancy chances with in vitro fertilization (IVF).ObjectiveTo prospectively compare a morphologic oocyte grading system to standard day-3 morphologic embryo assessment.Design, Setting, PatientsWe prospectively investigated in a private academically-affiliated infertility center 94 consecutive IVF cycles based on 6 criteria for oocyte quality: morphology, cytoplasm, perivitelline space (PVS), zona pellucida (ZP), polar body (PB) and oocyte size, each assigned a value of -1 (worst), 0 (average) or +1 (best), so establishing an average total oocyte score (TOS). Embryo assessment utilized grade and cell numbers of each embryo on day-3 after oocyte retrieval. Clinical pregnancy was defined by presence of at least one intrauterine gestational sac.InterventionsStandard IVF cycles in infertile women.Main Outcome MeasuresPredictability of pregnancy based on oocyte and embryo-grading systems.ResultsAverage age for all patients was 36.5 ± 7.3 years; mean oocyte yield was 7.97± 5.76; Patient specific total oocyte score (PTOS) was -1.05 ± 2.24. PTOS, adjusted for patient age, was directly related to odds of increased embryo cell numbers (OR 1.12, P = 0.025), embryo grade (OR 1.19, P < 0.001) and clinical pregnancy [OR 1.58 (95%CI 1.23 to 2.02), P < 0.001]. Restricting the analysis to day three embryos of high quality (8-cell/ good grades), TOS was still predictive of clinical pregnancy (OR 2.08 (95%CI 1.26 to 3.44, P = 0.004). Among the 69 patients with embryos of Grade 4 or better available for transfer 23 achieved Clinical Pregnancy. When the analysis was restricted to the 69 transfers with good quality embryos (≥ Grade 4) the Oocyte Scoring System (TOS) (AUC±SE 0.863±0.044, oocyte score) provided significantly greater predictive value for clinical pregnancy compared to the embryo grade alone (AUC 0.646 ± 0.072, embryo grade) p = 0.015.ConclusionsOocyte-scoring, thus, provides useful clinical information especially in good prognosis patients with large numbers of high quality embryos. This finding appears of particular importance at a time when many IVF centers are committing sizable investments to closed incubation systems with time-lapse photography, which are exclusively meant to define embryo morphology.

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Similar pregnancy outcomes from fresh and frozen donor oocytes transferred to gestational carriers: a SART database analysis isolating the effects of oocyte vitrification

Kloos,

Burks,

Purdue-Smithe

et al. 2024

J Assist Reprod Genet

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Developmental potential of embryos from intracytoplasmic sperm injection cycles containing fragmented oocytes

Cited by 3 publications

References 12 publications

Oocyte morphology and embryo morphokinetics in an intra-cytoplasmic sperm injection programme. Is there a relationship?

Oocyte morphology and embryo morphokinetics in an intra-cytoplasmic sperm injection programme. Is there a relationship?

Oocyte Scoring Enhances Embryo-Scoring in Predicting Pregnancy Chances with IVF Where It Counts Most

Similar pregnancy outcomes from fresh and frozen donor oocytes transferred to gestational carriers: a SART database analysis isolating the effects of oocyte vitrification

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